Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 82 for “"Viral DNA"”.
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Structure-Property-Transfection Relationships in Polycation-mediated Non-viral DNA Delivery
Non-viral gene delivery agents, such as cationic polyelectrolytes, are attractive replacements to viruses due to the absence of potential immunogenic risk and the ability to tune their macromolecular structure. Although non-viral vectors possess numerous design advantages, several investigators …
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An examination of the role of IFI16 in detecting viral DNA in human immortalised keratinocytes
The presence of exogenous DNA in the cytosol results in the activation of the DNA sensor cyclic GMP-AMP synthase (cGAS). cGAS produces the second messenger cyclic GMP-AMP (cGAMP) which binds to and activates the endoplasmic reticulum adapter STimulator of INterferon Genes (STING). Activated STING …
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Widespread Phosphorylation of H2AX by Adenovirus Requires Viral Genome Replication and the E1B-55k or E4orf3 Proteins
Adenovirus infection activates cellular DNA damage response and repair pathways. The viral E1B-55K and E4orf3 proteins prevent recognition of viral genomes as a substrate for DNA repair by targeting members of the sensor complex composed of Mre11/Rad50/NBS1 for degradation and relocalization, as …
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Structure and Function Study of Phi29 DNA packaging motor
… powerful nanomotor is employed by the tailed dsDNA virus to package the genome into a preformed protein shell during the process of replication. The bacteriophage phi29 is an excellent model for investigating the viral DNA packaging mechanism. The phi29 DNA packaging motor is composed of three …
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The Role of Viral Protein pUL21a in Human Cytomegalovirus Infection
The double-stranded DNA virus human cytomegalovirus: HCMV) is a ubiquitous human pathogen that causes severe disease in immunocompromised individuals and neonates. The biology of HCMV remains largely undefined and functions of many of the roughly 200 putative HCMV genes are still unknown. One of …
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Sensing Foreign DNA: The Role of DNA-PKcs in Human Anti-viral Innate Immunity
… molecular patterns (PAMPs), such as viral genomic DNA, which is a major PAMP during DNA virus infection. Viral DNA sensing leads to the activation of the STING-TBK1-IRF3 signalling axis and the production of type I interferon. Previously, our work identified the non-homologous …
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Attempts to localize the replicative form of polyoma virus DNA in mouse embryo cells
In order to localize replicating polyoma viral DNA (Py II*), a nuclear extraction technique is chosen and then slightly modified in order to rapidly yield purified nuclei in which the polyoma viral (Py) DNA is intact. A method is also developed which breaks the nuclear membrane, but not the Py DNA …
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Construction of an HIV-1 subtype C ventor system for phenotypic drug resistance studies
… (RT) and protease (PR) gene as targets of antiviral therapy has led to the discovery of various major, minor and polymorphic mutations harbored thereof. There are mutations in HIV-1 subtype B strains that develop only under drug pressure leading to ARV drug resistance. These mutations have been …
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Demonstration of new subtypes of adenovirus 7 in South Africa, and probing oesophageal carcinoma cell lines for evidence of adenovirus or of other oncogenic viruses
… adenovirus species 7; 2. Search for a possible viral aetiology in oesophageal carcinoma. Sixteen laboratory isolates of adenovirus species 7, isolated in South Africa between 1975 and 1986, were characterized by restriction endonuclease analysis of their DNA genomes. Virus was propagated in …
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Molecular analysis of herpes simplex virus type 1 latency in experimentally infected mice / Barry Slobedman.
… whether latent HSV genomes are a result of viral DNA amplification in the PNS during the establishment phase and to investigate the relationship between HSV DNA copy number and viral transcriptional activity during latent infection of the PNS. In order to map the distribution of viral …
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An investigation of genome-wide promoter region cytosine-phosphate-guanine (CpG) Island methylation profiles in patients with chronic hepatitis B virus infection
… antigens into the human genome. The integrated viral DNA disrupts gene function resulting in physiological changes that cause liver disease. The viral inserts are inactivated through methylation. This is a protective innate response driven by human DNA methyltransferases triggered by the …
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Pseudovirions in the Study of Papilloma- and Polyomaviruses
The papilloma- and polyomaviruses are small DNA viruses that infect humans. Some members of these virus families can cause cancer in experimental animals and some are also established as carcinogenic to humans. Detection of specific antibodies to these viruses allows tracking past and present …
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Murine Cytomegalovirus Encodes Proteins that Regulate Viral Late Transcription
… (including AIDS patients), and is the leading viral cause of congenital birth defects. Murine Cytomegalovirus (MCMV) is the primary surrogate model for HCMV, and resembles the human virus with respect to virion structure, genome organization, gene expression, tissue tropism, and clinical …
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Characteristics of an Endonuclease Associated with Adenovirus Types 2 and 12
… infects hamster cells. The metabolism of the DNA of these adenoviruses was determined in both human cells and hamster cells. The adenovirus DNA, both the parental DNA and the newly synthesized DNA, is found as three species within the cells. The species which sedimented rapidly is mainly …
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Isolierung und Charakterisierung von Iridoviren
… length profiles from digestion of purified viral DNA with the endonucleases EcoRI, BamHI, and HindIII showed marked differences from the profiles of iridoviruses of lower vertebrates (genus Ranavirus), e.g., Rana esculenta Iridovirus and Frog virus 3. Restriction enzyme digests with the …
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Identification and Characterisation of Human Cytomegalovirus-Mediated Degradation of Helicase-Like Transcription Factor
… host factors that are important in innate antiviral immunity in order to infect successfully. To systematically identify host proteins targeted for early degradation by human cytomegalovirus (HCMV), the lab developed orthogonal screens using high resolution multiplexed mass spectrometry. Taking …
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Investigation of the immunostimulatory activity and vaccine potential of lipid encapsulated plasmid DNA and oligodeoxynucleoties
DNA vaccines offer unique promise as a means of generating immunity against infectious and malignant disease. Unfortunately a number of obstacles, including rapid degradation of naked plasmid DNA (pDNA), poor cellular uptake by antigen presenting cells (APCs) and subsequent low levels of gene …
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Purification and Characterization of Simian Virus 40 Large T Antigen
Simian Virus 40 is a small DNA virus which can replicate normally following productive infection of permissive monkey cells, transform various cell lines of different species in tissure culture, and induce tumor formation following injection into newborn hamsters. Large T antigen is one of two …
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