Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"Vinculin"”.
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Dissecting interactions within focal adhesions: studies on vinculin
Vinculin and paxillin are proteins that localise to focal adhesions. Focal adhesions are<br/>specialised sites of cell attachment between the cytoplasmic side of the cell membrane and<br/>the extracellular matrix. They consist of receptors that link extracellular matrix ligands to<br/>the actin …
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EFFECTS OF RESTRICTIVE DISULFIDE BONDS IN THE VINCULIN TAIL DOMAIN
The C-terminal domain of vinculin, a protein of adherens junctions, is thought to undergo conformational changes upon binding actin or acidic phospholipid. For the present study, cysteine residues were inserted to form disulfide bonds to prevent motion in the arm (residues 1048-1066), strap …
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Mechanotransduction by talin : a molecular dynamics study of force-induced recruitment of vinculin to a focal adhesion complex
… understood. One example is the recruitment of vinculin to reinforce initial contacts between a cell and the extracellular matrix due to tensile force. Talin, an essential structural protein in the adhesion, contains the N-terminal five-helix bundle in the rod domain with a known cryptic …
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Diverse functions for intern associated proteins in Drosophila adult muscle
… detachment. However, the IAPs FAK, RSU1, tensin, vinculin and zyxin are not required for viability or embryonic muscle attachment. Furthermore, FAK, RSU1, tensin and vinculin have been observed to localise to muscle attachment sites in Drosophila, indicating that they have some function in muscle …
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TEMPERATURE VARIATION IN MARQUETTE, MICHIGAN, CONCERNING SKELETAL MUSCLE DEGRADATION PATTERNS FOR POSTMORTEM INTERVAL DETERMINATION
… of temperature on the degradation patterns of vinculin, a skeletal protein, and the levels of essential metals in human skeletal muscle. Calcium concentrations were also observed for evidence of calpain activation, which degrades vinculin. Once observations of vinculin degradation and calpain …
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Identifying novel roles for basement membrane-associated proteins in the adult Drosophila intestinal epithelium
… proteins including the conserved mechanoeffector Vinculin. I contributed to a project investigating the role of Vinculin in the intestinal epithelium, where *vinculin* mutants show increased intestinal stem cell proliferation and accelerated progenitor cell differentiation. I used scanning …
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Force-dependent changes in alpha-catenin conformation
… of α-catenin to the actin-binding protein vinculin and to the actin cytoskeleton itself. This regulation is accomplished through distinct conformational changes in the α-catenin central modulatory (M) domain and the C-terminal actin-binding domain (ABD), respectively. Force-induced …
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Development of a Generalizable Assay for Probing the Effects of Mechanical Force on the Function of Fluorescent Proteins within Molecular Tension Sensors
… of cell generated forces on FP function within vinculin-based MTSs. We chose vinculin as our model system as vinculin is a well-studied mechanosensitive protein, known to play a critical role in force transmission inside cells. Additionally, the vinculin tension sensor (VinTS) has been …
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Multi-Modal Regualtion of Actin Networks
… adhesion actin-binding proteins, α-catenin and vinculin, reveals α-catenin directly senses force on actin, while vinculin does not. Near atomic-resolution cryo-electron microscopy structures of both proteins bound to Factin, structure-guided protein engineering, and ongoing nuclear magnetic …
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VE-cadherin mechanotransduction signaling and its regulation by extracellular matrix
… also triggers the recruitment of vinculin and actin to cadherin complexes, to reinforce intercellular adhesions. Studies in this thesis investigated the underlying signaling pathways and the impact of the extracellular matrix environment on both barrier protective and barrier …
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Stress Fiber Formation and Migration Are Novel PGR-Dependent Pathways Initiated in the Granulosa Cells of the Ovulating Follicle
… ovulation. Two key PGR-regulated genes, Jam3 and vinculin were investigated further to establish a role in oocyte release. Vinculin, a stress fiber protein important for cell-ECM connection and collective cell migration, was upregulated following the LH surge and localised to leading edge-like …
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Purification and Activity of the PP60SRC Protein Kinase
… in vitro including tubulin, actin, vinculin, myosin, and filamin. Other proteins including the 34 kD phosphoprotein identified by Radke and Martin (1979) and cytosolic malate dehydrogenase were also phosphorylated by pp60<sup>src</sup> Further biochemical characterization of the …
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Tyrosine phosphorylation and cytoskeletal tension regulate the release of fibroblast adhesions
… contracted with talin, $\alpha$-actinin and vinculin localized diffusely throughout the cell. Integrin containing tracks of variable intensity outline the regions where cells had resided before they detached from the substratum. At lower ATP concentrations (0.5-5mM) the cells remain spread; …
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Evaluating the effects of acoustic stimulation on fibroblast cell migration and muscle fibre formation.
… the mean cell migration distance (µm), actin and vinculin organisation, cell viability, muscle fibre diameters and the effects of temperature were investigated. The results show that 100Hz acoustic vibration can enhance cell migration, wound healing in LL24 and L929 cells and possible increase …
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Ethanol Impairs Mechanisms of Macrophage Phagocytosis and Cytokine Production
… of the adhesion molecules paxillin and vinculin, forming a structure we term "phagosomal adhesion". Ethanol did not alter paxillin phosphorylation during phagocytosis via the FcgammaR-pathway but suppressed vinculin phosphorylation, suggesting a defect in phagosomal adhesion maturation. …
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Epidermal growth factor receptor is an essential component in E-cadherin force-transduction complexes
… including adaptive cell stiffening, actin and vinculin recruitment at stressed junctions, were analyzed with E-cadherin mutants. The results demonstrated that without E-cadherin/EGFR physical complexes, cells were unable to activate EGFR and integrin in response to force, resulting in a lack of …
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Characterisation of cardiac protein changes post death: pilot study
… in native cardiac troponin Cardiac troponin I, vinculin, and eukaryotic elongation factor (eEf1a) and their degradative products were evaluated in cardiac tissue using Western blot(WB) assay from decedents recruited at the Salt River Mortuary(SRM), Cape Town. The native and degradative products …
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THE IN-VIVO IMPACT OF SMPX MUTATIONS DURING DEVELOPMENT AND DISEASE
… uncovering that the V-shaped patterning of Vinculin, the only known interactor of Smpx, was completely misshaped in the myotendinous junctions; additionally, we pinpointed that a small proportion of Vinculin became mislocalized within the myotome. More recently, different SMPX missense …
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Serine 910 Phosphorylation of Focal Adhesion Kinase Is Critical for Costamere Assembly
… mutant decreased the interaction of paxillin and vinculin within costameres. This interaction was important in stabilizing α-actinin within the protein complex, as evident by a decrease in <sub>k</sub>FRAP for α-actinin-YFP. The S910A-FAK mutant also blocked ET-1 induced NRVM spreading and …
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The Role of Ca²⁺/CaM-Stimulated Phosphodiesterase 1C in Vascular Smooth Muscle cells of the Synthetic/Activated Phenotype
… the expression of other markers, including PKG, vinculin, and beta-1 integrin were unaffected. Treatment with PDE1C siRNA also reduced cell migration, proliferation, and the ability of HASMCs to bind extracellular matrix proteins. These effects were mediated without any major changes in global …
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