Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 31 for “"Video Microscopy"”.
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High-speed video microscopy in optical tweezers
… at high enough frame rate. Recent advances in video camera technology allow the collection of images at several kHz. However, there has been little use of high-speed cameras in optical tweezers, partly due to data management problems and affordability. This the- sis presents seven experiments …
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Multiple particle tracking to assess the microstructure of biological fluids
… in soft matter sciences such as biophysics. Video microscopy particle tracking is an easy technique to implement experimentally. Movies of the fluctuating particles in a sample are recorded and analyzed off-line using custom algorithms. For this reason, it is widely used in studies of soft …
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Polymer and sphere diffusion in confinement
We use video microscopy and particle tracking to study the diffusion of both colloidal spheres and polymers in slit microchannels. The molecules are allowed to sample the entire height of the microchannel. We find that the sphere data agrees well with a gap-averaged Oseen linear superposition …
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First evidence of skelemin, a myosin-associated protein, in smooth muscle and its involvement in cell adhesion, and, The role of the cell cycle in cell type choice during mammalian development
… as well as cell-substrate adhesion. Time lapse video microscopy was used to analyze the role of the cell cycle in cell type choice during mammalian development. Discussed are methods employed to improve resolution for accurate analysis.
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Examination of a system for the mechanistic study of tumor cell-platelet interactions under well-defined flow conditions
… of the microvasculature. Epi-fluorescence video microscopy utilizing a cooled CCD camera and digital image processing were used to quantify the dynamic adhesion and aggregation of platelets. Varying the exposure time of the monolayer to the 445 nm excitation wavelength revealed that …
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Prognostication and disease monitoring in fibrotic interstitial lung disease
… explored the use of side-stream darkfield video-microscopy to quantify endothelial glycocalyx (EG) health in patients with f-ILD and a SPECT-CT scan of V3 integrin activity was used to image angiogenesis in f-ILD. Results The systematic review identified numerous high quality studies …
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A model for sample stacking in microcapillary DNA electrophoresis
… study, images of sample injection obtained using video microscopy showed sharp sample stacking peak at the trailing edge of the sample plug. This thesis examines the underlying physics that explain the behavior of DNA in microcapillary electrophoresis. A developed model captures the dynamics of …
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Measurements and models of electrically-evoked motion in the gerbil organ of Corti
… within the stimulus period using stroboscopic video microscopy. Animations of this motion were created, and the displacement magnitude and phase for each structure were calculated using two dimensional cross-correlation. With these techniques we were able to detect displacements as low as ten …
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Digitale Bildverarbeitung schneller Partikelbewegungen in kolloidalen Suspensionen
… These results demonstrate, that high speed video microscopy is well suited to examine rheological mechanisms in colloidal suspensions and therefore is an important addition to the established scattering methods.
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DNA-colloid systems and micro-rheology
… pH dependence on their coverage. From analysing video microscopy trajectories, PLL-PEG coated beads sedimented onto A-DNA brushes are found to be significantly more diffusive. In Chapter 5, we int roduce an entirely new DNACC system, the functionalised fd bacteriophage, where high aspect ratio …
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Building capacity for diagnosis of Primary Ciliary Dyskinesia in South Africa: a descriptive study
… nasal nitric oxide (nNO), nasal brushings for video microscopy of ciliary beat; transmission electron microscopy (TEM), immunofluorescence (IF) of ciliary protein antigens and genotyping. Results: Thirty-three participants (31 children; 2 adults) were enrolled July 2022 to July 2023 [median …
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Effects of oxidative stress and neuroprotection in apoptosis in neuronal cell models
… (ETC), and cell morphology by fluorescence and video microscopy, both of which showed OS to cause decreased viability and changes in morphology. Levels of intracellular peroxide production, and changes in glutathione and carbonyl levels were also assessed, which showed OS to cause increases in …
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A Systems Approach to the Modeling and Control of Molecular, Microparticle, and Biological Distributions
… (coupled with inverse modeling) and video microscopy (coupled with image analysis) are used to determine the droplet/particle size distribution. A high resolution finite volume algorithm is used to numerically solve the population balance equation to describe the particle size …
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Cell-cycle dependent motility of Chlamydomonas reinhardtii and its collective motion in response to a Hagen-Poiseuille flow
… against gravity were measured with high-speed video microscopy with Chlamydomonas reinhardtii cultures highly synchronised to a diurnal cycle. A simulation of gravitactic cell trajectories was developed to assist the statistical analysis of the cell trajectories from the images, which subject …
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Removal of Bacteria from Solids by Bubbles: Effect of Solid Wettability, Interaction Geometry, and Liquid–Vapor Interface Velocity
… that the numbers of bacteria were monitored via video microscopy throughout the collision, so we were able to explore the mechanism of bacteria removal. When a bubble collides with a solid, an air–water interface crosses the solid twice, and we were able to distinguish the effects of the first …
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Identification of genes involved in gliding motility and proteomic analysis of spore inner membrane proteins in Clostridium perfringens
… type cells, the hypermotile cells are longer and video microscopy of their gliding motility suggests they form long, thin filaments that move rapidly away from a colony, analogous to swarmer cells in bacteria with flagella. Whole genome sequencing analysis showed that both mutants have mutations …
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Vagal Integration of Cardiopulmonary Regulation
… Pacific oysters using a novel, non-invasive video-microscopy method developed to assess HR/HRV during acute temperature changes in oysters acclimated to 22°C or 10°C. Acclimation to 10°C reduced the incidence of asystole and increased HRV vs. warm-temperature acclimation. HRV increased as …
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Nonlinear Dynamical Analysis Of Microvascular Red Blood Cell Flow
… to acquiring and analyzing time series data.;Videotape records of capillary blood flow in-vivo in the rat extensor digitorum longus muscle, were made for up to 60 minutes using in-vivo video microscopy. Time series records of RBC velocity were generated, and chaotic time series analysis was …
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The Role of Protein S-glutathionylation on Ca2+ Signaling in Cultured Aortic Endothelial Cells
… Ca2+ indicator, fura-2 and time-lapse video microscopy. Low concentrations (50, 100 ¿¿M) of diamide reversibly increased spontaneous Ca2+ oscillations, whereas high concentrations (250, 500 ¿¿M) of diamide caused an immediate, synchronized Ca2+ oscillation in essentially all cells …
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Liquid manipulation using engineered carbon nanotube surfaces
… roll-off on Morpho aega butterfly wings. By video microscopy, we reveal that extreme deflections of the individual scales are responsible for directional adhesion. Inspired by this finding, I demonstrate a synthetic scale surface with stiffness-tunable anisotropic droplet adhesion, fabricated …
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