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Showing 1 to 17 of 17 for “"Vibrio vulnificus"”.
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Control of Vibrio vulnificus and Vibrio parahaemolyticus in oysters
Vibrio vulnificus and Vibrio parahaemolyticus are gram-negative halophilic bacteria found in the natural aquatic environment. V. vulnifcus and V. parahaemolyticus have been implicated in foodborne illness and can cause gastroenteritis that has been associated with consumption of raw or undercooked …
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Application of Anti-H Monoclonal Antibodies to Develop Rapid Immunoassay for Vibrio vulnificus
… before releasing the lots. In the case of Vibrio vulnificus (V. vulnificus), conventional methods are available to identify and enumerate this pathogen in oysters, but they are labor-intensive and time consuming. To maintain a constant supply of safe oysters, methods need to be developed …
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Monoclonal and phage-displayed antibodies as lipopolysaccharide serotyping reagents and their use in typing colony variants of Vibrio vulnificus
Vibrio vulnificus is a Gram-negative organism that is found in estuarine environments. The bacterium is capable of causing gastroenteritis, wound infections, and septicemia, often resulting in death for susceptible individuals. Monoclonal antibodies (MAbs) to five lipopolysaccharide (LPS) …
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Survival of Vibrio vulnificus and Escherichia coli in artificially and naturally infected oyster (Crassostrea virginica) tissues during storage in spray- and immersion-type live holding systems
… systems on the survival of Escherichia coli and Vibrio vulnificus within the oysters. The effects of physiological stress imposed on oysters, as a result of interstate shipping, were examined by monitoring the level of E. coli in these oysters during storage in a spray tank. The survival rates of …
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An Evaluation of the Role of Temperature on the Safety and Quality of Raw Shellstock Oysters and Bluefish
… time before proliferation of pathogenic flora (Vibrio vulnificus) made the product unsafe. Spoilage was evaluated through pH measurements of a homogenate of the shucked meat and liquor. The olfactory acceptability of the raw oysters was evaluated in concert with the microbial and chemical …
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Improved Detection Methods for Selected Vibrio Species in the Marine Aquatic Environment
<p><em>Vibrio vulnificus</em>, <em>Vibrio cholerae</em>, and <em>Vibrio parahaemolyticus</em> are the three most commonly encountered human pathogens in the <em>Vibrio</em> genus. They are frequent agents of foodborne illness contracted from seafood. The work presented here focuses on three aspects …
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Application of NMR spectroscopy to interrogate the structures of bacterial protein toxin membrane localization domains and amphotericin B
… repeats-in-toxins (MARTX) toxin expressed by Vibrio vulnificus. The function of these MLDs is to localize the toxin to membranes, which aid in the targeting of the catalytic domains. The study reveals that these two toxins form 4-helix-bundle motifs in solution, consistent with X-ray crystal …
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Characterization of the Diverse Substrate Specificities and Biological Roles of Polyamine Biosynthetic Enzymes in Microorganisms
… for L-ornithine, while the enzyme from Vibrio vulnificus (VvL/ODC) had dual specificity functioning well on both L-ornithine and L-lysine. The X-ray structure of VvL/ODC was solved in the presence of the reaction products putrescine and cadaverine to 1.7 and 2.15 Å, respectively. The …
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Plastics and Microplastics as Vectors for Bacteria and Human Pathogens
… for bacteria, with particular focus on<em> Vibrio spp</em>., including the human pathogens, <em>Vibrio cholerae</em>, <em>Vibrio parahaemolyticus</em>, and <em>Vibrio vulnificus</em>.</p> <p>Colonization experiments were set up in a tributary of the lower Chesapeake Bay to follow <em>Vibrio …
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Wirkung des Ballastwasser-Biozids Acrolein auf verschiedene pathogene Bakterienstämme in Meer-, Brack- und Süßwasser
… Pseudomonas aeruginosa, Aeromonas hydrophila, Vibrio parahaemolyticus, Vibrio alginolyticus und zwei Stämme Vibrio vulnificus. Basierend auf der ScanMIC-Methode (Rahman et al, 2003) wurden Mikrotiterplatten mit Bakterienflüssigkultur, Acrolein und Probenwasser unterschiedlichen Salzgehaltes …
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Application of Alternative Technologies to Eliminate <i>Vibrios </i> spp. in Raw Oysters
… gamma irradiation were applied to inactivate <i>Vibrio vulnificus</i> (MO624) and <i>Vibrio parahaemolyticus</i> (O3:K6 TX2103) in pure culture and in inoculated live oysters. <i>Vibrio</i> pure culture and inoculated oysters were exposed to pressures of 207 MPa (30 kpsi) to 552 MPa (80 kpsi) for …
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Health impacts of climate change in the older population
… and food-borne diseases was 2.8 times higher for Vibrio vulnificus septicemia and 1.3 times higher for shigellosis in the elderly. Older women were more vulnerable to scrub typhus than their male counterparts and the most affected region to scrub typhus was in the order of Jeollabuk-do, …
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Multi-Scale Modelling of Host-Microbe Interactions: From Cell Membranes to Multi-Cellular Gut Models
… bacteria, namely the opportunistic pathogen Vibrio vulnificus (V. vulnificus) known for its severe disease including gastroenteritis, wound infection and sepsis. Focus is given to establishing intestinal cell-derived membrane models, studying cell line susceptibility by monitoring barrier …
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Effect of storage temperatures on the microbiological quality, safety, and viability of quahog clams, Mercenaria mercenaria
… pathogens, Vihrio parahaemolyticus and Vihrio vulnificus. Aerobic plate counts (APCs), coliforms, and fecal coliforms were determined to define the level of quality. During the summer, mesophilic APCs containing 2% NaCI ranged from 104 to 108 CFU/g, and during the winter, mesophilic APCs with …
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Survival of Vibro vulnificus and other Vibrios in raw oysters (Crassostrea virginica) during processing in Virginia and cold storage
… objective of this research was to determine if Vibrio populations, specifically V. vulnificus were affected in oysters by the processing methods employed in Virginia. This study was conducted between July and September in 1995 and during the month of August of 1996 when water temperature was …
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Functional analysis of the role of alternative sigma factors in Vibrio parahaemolyticus host pathogen interactions
Vibrio parahaemolyticus, a ubiquitous Gram-negative marine bacterium, is the leading cause of bacterial seafood borne gastroenteritis in humans. This organism must adapt to both host and marine environmental stresses. One mechanism bacteria employ to respond to rapid environmental changes is the …