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Showing 1 to 20 of 28 for “"Vibrio fischeri"”.
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Exploring the Physiological Role of Vibrio fischeri PepN
<p>The primary contributor to Vibrio fischeri aminopeptidase activity is aminopeptidase N, PepN. Colonization assays revealed the pepN mutant strain to be deficient at forming dense aggregates and populating the host’s light organ compared to wildtype within the first 12 hours of colonization; …
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Characterization of the Broad-Spectrum Racemase, BsrF, in Vibrio fischeri
… as growth substrates. A Bsr was identified in Vibrio fischeri, BsrF, that appears to be localized to the periplasm. The main objective of this study was to determine the substrate range of BsrF for proteinogenic L-amino acids and to explore functional roles for BsrF. BsrF racemized D-Arg, …
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Identifying Novel Factors Involved in Biofilm Formation by Vibrio Fischeri
… utilized as a model system the marine bacterium Vibrio fischeri, which must be competent to form a biofilm to colonize its squid host Euprymna scolopes. Biofilm formation by V. fischeri requires the syp polysaccharide locus, as well as the sensor kinase RscS and the response regulators SypG, …
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Analysis of the Regulons Controlled by Transcriptional Regulators LuxR and LitR in Vibrio fischeri
… first observed in the symbiotic marine bacterium Vibrio fischeri and this system is one of the best studied quorum sensing systems. Two-dimensional sodium dodecyl sulfate-polyacrylamide (2D-SDS) gel electrophoresis analysis previously identified several non-Lux proteins in V. fischeri MJ-100 whose …
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The quorum-sensing regulation of Vibrio fischeri : novel components of the autoinducer/LuxR regulatory circuit
In the marine bacterium Vibrio fischeri two intercellular homoserine-Iactone signal molecules (luxI-dependent 30C6-HSL and the ainS-dependent C8-HSL) and the transcriptional activator LuxR regulate the luminescence system in a cell-density dependent manner by a process termed quorum sensing. In …
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The quorum-sensing regulation of Vibrio fischeri : novel components of the autoinduce/LuxR regulatory circuit
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 1999.
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Experimental Methods in Support of the Development of a Computational Model for Quorum Sensing in Vibrio fischeri
… data available on the quorum sensing system in Vibrio fischeri allows the development of a systems biology approach to undertake a spatial and dynamical analysis of the regulation throughout the population. The quorum sensing regulated lux genes are organized in two divergent transcriptional …
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Characterization of the Novel Response Regulator Sype: A Dual Regulator of Biofilm Formation and Colonization in Vibrio Fischeri
… tissues. The symbiosis between the bacterium Vibrio fischeri and its animal host Euprymna scolopes provides a natural model system for investigating the impact of biofilms on host infection. In particular, V. fischeri produces a specific biofilm to successfully initiate host colonization.</p> …
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Multi-tiered Regulation of luxR Provides Precise Timing and Maintenance of the Quorum Sensing Response of Vibrio fischeri
The quorum-sensing response of Vibrio fischeri involves a complex network of genes (encoding regulatory proteins as well as sRNAs), that govern host-association and production of bioluminescence. A key regulator of this system is LuxR, which is the transcriptional activator of the lux operon as …
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Mutant analysis of luminescence and autoinduction in a marine bacterium
The marine symbiotic bacterium Vibrio fischeri is striking for its ability both to emit light and to dramatically regulate light emission using a cell-to-cell signalling mechanism called autoinduction. The latter is mediated by a signal molecule called the "autoinducer". The mechanistic bases of …
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Novel Applications of Biolayer Interferometry to Investigate Molecular Interactions
… to assign the role of protein function in both Vibrio fischeri and Pseudomonas aeruginosa. Each aim of this research project will demonstrate novel applications of BLI technology to further characterize molecular interactions in an inexpensive, time-efficient manner that produces real-time data. …
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Study of cell-cell communication using 3D living cell microarrays
… looked at a model "quorum sensing" system in Vibrio Fischeri, which operates by the emission and detection of a small chemical signal, an acyl-homoserine lactone. Through synthetic biology, I have engineered plasmids which express "sending" and "receiving" genes. Bacteria containing these …
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A bioengineered approach for sustainable crude oil pollution treatment via entrapment, dispersal and removal using Nano-CarboScavenger
… by absorption alone. NCS exposure to Zebrafish, Vibrio fischeri, and MCF-7 were performed in vitro and were found to be remarkably un‐inhibitive with LD50: 8 g/L, EC50: 1.28 g/L and IC50: 4 g/L respectively. This far exceeds expected NCS exposure (ENE) of 1x10-5 g/L in bodies of water. Finally, …
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Caracterización de material de dragado optimizando un método
… la luminiscencia en un apoblación de bacterias Vibrio fischeri como medida inicidal de la toxicidad de los sedimentos, el desarrollo anormal de larvas del erizo de mar Paracentrotus lividos expuestas al lixiviado, y la mortalidad de anfípodos tras la exposición a los sedimentos en bruto. Estos …
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Assessment of the Efficacy of a Constructed Wetland to Reduce or Remove Wastewater Effluent Estrogenicity and Toxicity Using Biomarkers in Male Fathead Minnows (Pimephales Promelas Rafinesque, 1820)
… was compared with Ceriodaphnia dubia and Vibrio fischeri toxicty tests on this effluent. Biomarkers of fish health were somewhat less sensitive than C. dubia test endpoints, but more sensitive than V. fisheri.
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Signaling between Two Sensor Kinases Controls Biofilms and Host Colonization in a Bacterial Symbiont
… response regulator, RR). The marine bacterium Vibrio fischeri uses an elaborate TCS phosphorelay containing a hybrid SK, RscS, and two RRs, SypE and SypG, to colonize its natural squid host, Euprymna scolopes. This TCS pathway regulates V. fischeri's ability to form a biofilm, or a community of …
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Development of Modified Reverse Osmosis Membrane with Polymers/Graphene Oxide for Scaling and Biofouling Mitigation
… coverage significantly, with ESPA2-PMA reducing Vibrio fischeri biofilm growth by 40% compared to unmodified membranes. The addition of GO into the PMA matrix further enhanced biofilm resistance, reducing biofilm formation by over 60%. Notably, this is the first study to demonstrate the …
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The Influence of Changes In theMolecular Structure of N-Acylhomoserine Lactones On the Complexation and Dna-Binding of Luxr Protein
… the first identified quorum-sensing system, the Vibrio fischeri LuxI/LuxR paradigm, was limited to molecular genetic approaches due to the formation of inclusion bodies when overexpressing LuxR in Escherichia coli. The addition of its native autoinducer, N-(3-oxohexanoyl)-homoserine lactone …
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Role of region 4 of the sigma 70 subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing
… in the bioluminescent marine bacterium Vibrio fischeri. The cell-density dependent activation of the luminescence (lux) genes of V. fischeri relies on the formation of a complex between the autoinducer molecule, N-(3-oxohexanoyl) homoserine lactone, and the autoinducer-dependent …
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Transcriptional Control during Quorum Sensing by LuxR and LuxR Homologues
… manner. The quorum sensing system of Vibrio fischeri activates the luminescence (lux) operon when the autoinducer signaling molecule (N-3-oxohexanoyl homoserine lactone) is recognized and bound by the activator protein LuxR. LuxR subsequently binds to the lux box centered at à 42.5 bp …
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