Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Viable but non-culturable"”.
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Development of a Flow Cytometry Method for the Identification and Enumeration of Enterococcus faecalis
… the potential of quantifying cells that are in viable-but-non-culturable (VBNC) state. Initial field test using natural seawater samples spiked with <em>E. faecalis</em>, demonstrated high accuracy (98-120 % recovery rate) and precision (6-10% CV) of the method to detect <em>E. faecalis</em>. …
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Smart microscopy methods for quantitative microbiology
… this field by enabling high-throughput, non-invasive, time-lapse observations of cellular processes at the single-cell level. However, despite its power, this method is not without limitations. Optical artefacts distort quantitative measurements, obscuring the accuracy and precision of …
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Limitations to Use Copper as an Antimicrobial Control of Legionella in Potable Water Plumbing Systems
… levels of natural organic matter (NOM) in distributed water are predicted to be important controlling factors. The type of sacrificial anode rod material employed in water heaters was also identified as an underappreciated factor, which directly affects pH, evolution of hydrogen gas as a …
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Influence of Physiological State, Prolonged Dry Storage, and Passage through Simulated Digestion on the Survival and Gene Expression of Salmonella enterica sv. Tennessee
… suggesting entry of S. Tennessee into a viable but non-culturable state. Prolonged storage in dry milk powder was not associated with increased cross-protection to gastric stress. Increased expression of stress response genes rpoS and otsB correlated with survival, indicating cross …
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Density-shift Immunomagnetic Separation for Pathogen Retrieval from Complex Media
… sensitivity when the sample has a background of nontarget cells, proteins, and detritus. Reducing heterogeneity, removing inhibitory substances, and concentrating target cells by sample preparation could enable more rapid detection of pathogens from complex media, such as food samples. This …
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Novel techniques for the in situ detection of bacteria on salad leaf surfaces
… and quantify both indigenous leaf bacteria and viable human pathogens on salad leaves, without the need for recovery and culture, which may reduce the accuracy of quantification. The methods developed could then be used to quantify the number of indigenous and inoculated bacteria in situ on …
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Survival and recovery of Escherichia coli O157:H7 during starvation at sub-optimal temperatures
… of growth within a host and persistence in non-host environments. Environmental survival of E. coli O157:H7 is particularly important in New Zealand due to the increasing trend in notified clinical cases, with most being associated with rural environments rather than contaminated regulated …
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Characterising the microbial communities associated with the water distribution system of a broiler farm and their role in Campylobacter infection
… Different sources have been recognised, but their actual contribution to Campylobacter epidemiology remains a matter of debate. Water has rarely been identified as a possible source of Campylobacter contamination of chickens, but represents an understudied source. Research suggests that …
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Combining Conventional Tests and Terminal Restriction Fragment Analysis to Evaluate Microbial Quality of Raw Milk
… of milk and milk products. One factor that contributes to this lack of correlation is the insufficient knowledge of microbial communities in raw milk. In this work we aimed to evaluate modern molecular methods to complement traditional quality procedures that may eventually complement conventional …