Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Vacuum Infiltration"”.
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Attempts toward the expression in transgenic Arabidopsis thaliana of Esi47, a salt stress-induced gene encoding a protein kinase from Lophopyrum elongatum
… of A. thaliana, by tissue-culture and vacuum infiltration, were both attempted. A number of plant transformation constructs were made for gene promoter analysis. Expression constructs were also made and tested for the production of Esi47 protein in E. coli using the pATH11 trpE fusion …
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Nitrate reductase in apple trees
… The optimum pH of the assay medium was 7.5. Vacuum infiltration and the addition of 2% (v/v) n-propanol to the assay medium resulted in stimulation of nitrate reductase activity. The in vivo nitrate reductase assays for leaves and stems were linear for at least 60 minutes following an initial …
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Embryogenic response of potato anther culture to colchicine
… conducted to study various durations (0, 90 sec vacuum infiltration, 24, 48, and 72 h) of high colchicine treatment (200 mg/L) applied to <i>S. phureja</i> family DM 13-14 202 x ID 5. There was nearly significant difference among treatments (α = 0.05). Mean embryos per anther ranged from 0.96 to …
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Survival and Growth of attenuated Salmonella enterica serovars Newport and Typhimurium in Media Culture and Tomatoes
… inoculated with 7 Log CFU/g of each isolate via vacuum infiltration, surface spot inoculation, or diced inoculation. Populations of each strain were determined on Days 0, 1, 3, and 5. In media, there were few differences in overall growth and growth rates between mutant isolates and wild-type …
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Isolation and Characterization of the Soybean Major Latex Homologue Msg, and Analysis of Its Pod -Specific Promoter
… transformation into Arabidopsis by Agrobacterium vacuum infiltration. The full length promoter is needed for maximal expression in floral and fruit tissues, guard cells and nodes. Surprisingly, the minimal promoter of 650 bp is only functional in Arabidopsis when the nosT is replaced by the Msg …
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Isolation and Characterization of the Soybean Major Latex Homologue Msg, and Analysis of Its Pod-Specific Promoter
… transformation into Arabidopsis by Agrobacterium vacuum infiltration. The full length promoter is needed for maximal expression in floral and fruit tissues, guard cells and nodes. Surprisingly, the minimal promoter of 650 bp is only functional in Arabidopsis when the nosT is replaced by the Msg …
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Investigating mechanical properties of ordinary portland cement. Investigating improvements to the mechanical properties of Ordinary Portland Cement (OPC) bodies by utilizing the phase transformation properties of a ceramic (Zirconia).
… has also been introduced into OPC discs by vacuum infiltration methods developed for solutions and colloidal suspensions. Comparisons between OPC discs and the OPCtetragonal ZrO2 composites have been made on the basis of diametral compression strength, Young’s modulus, hardness and toughness …
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The Potential Interaction of Salmonella enterica and Ralstonia solanacearum in Tomato Plants
… the relationship between the two bacteria via vacuum infiltration inoculations of tomato fruit collected from commercial production fields on the ESV with S. Newport. Tomato fruit were collected from plants expressing symptoms of bacterial wilt (symptomatic) and plants not expressing bacterial …
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ENGINEERING THERMAL CONDUCTIVITIES FOR ENERGY APPLICATIONS
… a novel approach is proposed to develop vacuum insulation panels (VIPs) featuring multilayer structures using a flexible and cost-effective 3D printing process. By optimizing the number of interlayers in the VIPs, an effective thermal conductivity substantially lower than that of air is …
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Genetic Engineering of Black Cherry (Prunus serotina) for Reproductive Sterility and Insect Pest Resistance
… in our lab. The application of 15 minute vacuum infiltration, Agrobacterium concentration at OD600 of 1.0 or 1.5, and a 3-day co-cultivation were found to be optimal. Rooting of transgenic black cherry shoots was achieved at 30% using half-strength Murashige and Skoog medium supplemented …
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Using a Mammalian Virus to Create Plants for Site-Specific Transgene Insertion
A novel strategy for site-specific DNA transformation of plants has been proposed and the first component of the system developed. The proposed method overcomes the limitations of current techniques by providing a specific integration site for the insertion of transgenes using features of the …
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Self-healing of high temperature cured epoxy and composites
… agent. Amine microcapsules were prepared by vacuum infiltration of the target amine into hollow polymeric microcapsules. Epoxy microcapsules were prepared by an in situ polymerization method. Both types of capsules were incorporated into an epoxy matrix and recovery of mode-I fracture …
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An investigation of aspects of the nitrogen physiology of Helianthus annuus L
… fed either nitrogen source alone. 15N0 3 - vacuum infiltration of mature leaves on 10 week old plants showed that the products of nitrate assimilation were transported mainly to the capitulum. Even at this late stage of fruit filling, the root was shown still to be processing nitrogen as 26% …
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Soft rot Enterobacteriaceae-molecular detection and biocontrol under in vitro and in vivo conditions
… in 2018 via two methods of application (vacuum-infiltration and soil drench). No negative effect of the formulated phage-based cocktail was observed on plants, and treated potato tubers showed significantly better yield than untreated potatoes. The phage-based cocktail tested on onions …
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The Expression of Chikungunya Virus Envelope 2 Glycoprotein Variants in Nicotiana benthamiana for the Development of a Diagnostic Reagent
… via Agrobacterium-mediated small-scale 6 syringe-infiltration at different optical densities, OD600 = 1.0 and 0.5. To improve expression, both genes were co-infiltrated and co-expressed with a human chaperone proteins calreticulin (CRT) or calnexin (CNX), or a plant silencing-suppressor protein …
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Lead - Free Piezoelectric Based Magnetoelectric Composites
… anodizing. The PZT nanorods were synthesized by vacuum infiltration of PZT precursor solutions and exhibited uniform diameter of 90 nm and aspect ratio of 10 with vertical in respect to the Pt-Si substrate. The piezo-response of PZT nanorods showed good magnitude owing to the reduced clamping …