Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Vacuole Fusion"”.
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The regulation of yeast homotypic vacuole fusion by phosphatidic acid and diacylglycerol
Eukaryotic membrane fusion is a highly conserved process that is necessary to maintain cellular homeostasis. Ultimately, two membrane compartments are brought to each other, direct contact is established, and the bilayers and lumenal contents mix. In Saccharomyces cerevisiae, homotypic vacuole …
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Mutational analysis of the Vam3 transmembrane domain and its effects on vacuole fusion in Saccharomyces cerevisiae
Vam3 is a SNARE protein essential in yeast vacuole homotypic fusion. The specific role of the Vam3 trans-membrane domain (TMD) during vacuole fusion remains unclear. Previous work had shown that replacing Vam3 TMD with a prenyl lipid anchor (CCIIM) results in attenuated fusion and accumulation of …
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The regulation of yeast homotypic vacuole fusion and membrane trafficking events by sphingolipids, ergosterol, and lipid rafts
Membrane fusion is a conserved process that facilitates essential events like endocytosis, secretion, cell division and autophagy. Membrane fusion occurs at all organelles, and many of the fundamental processes required for fusion have been extensively studied. This process requires a minimal set …
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Polyphosphoinositide-derived signals in the regulation of vacuole membrane fusion and fission
… exchange membrane and protein cargoes. The yeast vacuole is a highly dynamic organelle that changes shape in response to both hypo and hyper-osmotic stresses and so has been used to study both membrane fusion and fission. Fusion is relatively well characterised but fission is not well understood. …
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The regulation of yeast homotypic membrane fusion by class C ABC transporters
… of eukaryotic cellular homeostasis requires the fusion of vesicle membranes that is accomplished by a SNARE-mediated mechanism. Membrane fusion is the merger of two lipid bilayers into one continuous membrane. Multiprotein complexes that have been conserved in eukaryotes carry out the basic …
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The Regulation of the Phosphatidate Phosphatase Gene <em>PAH1</em> and Its Regulatory Role on Cell Homeostasis
… genes needed for phospholipid synthesis, vacuole homeostasis, and lipid droplet formation. Due to its crucial role in lipid and overall cell homeostasis, this thesis aimed to elucidate the role and regulation of the <em>PAH1 </em>gene. In the first part of the study, we tried to evaluate …
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Novel lipid regulation of vacuolar fusion in saccharomyces cerevisiae
… terminal stage of trafficking is the membrane fusion of two distinct compartments leading to the mixing of contents. In order to regulate this process organelles are enriched with specific fusion proteins termed SNAREs. While the core fusion machinery is well understood, relatively little is …
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The role of the Dgk1 and Pah1 in lipid interconversion on saccharomyces cerevisiae vacuole physiology, homeostasis, protein sorting and fusion
A variety of factors are needed for the homotypic fusion of the yeast vacuole, including a number of proteins and regulatory lipids. The deletion of the phosphatidic acid (PA) phosphatase gene Pah1, which converts PA in diacylglycerol kinase (DAG), was previously shown to produce a fragmented …