Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"VSV G"”.
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MODIFICATION OF VESICULAR STOMATITIS VIRUS G PROTEIN FOR TARGETED GENE DELIVERY INTO PSCA-POSITIVE TUMOR CELLS
… cell. The Vesicular Stomatitis Virus G protein (VSV-G) is a well-liked choice for pseudotyping the retroviral vectors since it confers on the viral particle stability to allow concentration to high titers necessary for the clinical applications. However, the main drawback of VSV-G, the …
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Coarse-grained Modeling of Protein Dynamics using Elastic Network Models
… vesicular stomatitis virus glycoprotein G (VSV-G), which triggers the pH-dependent fusion of viral and host membrane, undergoes a large structural rearrangement. A striking difference between the two proteins is their shape. VSV-G contains weakly constrained protein segments, the fusion …
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Funktionelle Charakterisierung von Mitgliedern der p24-Proteinfamilie
… p23tail* eine fast 80ige Transporthemmung des VSV-G Proteins zur Zelloberfläche. Zudem hemmte die Mikroinjektion von Fab-Fragmenten, welche gegen die zytoplasmatischen Domänen von p24 Proteinen gerichtet waren, spezifisch den Transport von VSV-G Protein. Deshalb wird für alle untersuchten p24 …
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In vitro and In vivo Characterization of the Infection Efficiency of a Modified Retrovirus Envelope Glycoprotein for Targeting Gene Transduction
… mediated by vesicular stomatitis virus (VSV) G protein when each envelope protein is pseudotyped on MLV particles. To address the contribution of receptor characteristics on infection efficiency, HEK 293 cell lines stably expressing comparable cell surface levels of SSTR-2, SSTR-3 and …
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Engineering Viruses for Gene Therapy: Isolating and Characterizing Murine Leukemia Virus With Improved Stability
… including amphotropic, ecotropic, 10A1, and VSV-G. Lastly, saturation mutagenesis at the site of the beneficial mutation identified MLV mutants with infectivity half-lives of ∼24 h at 37°C, nearly a 4-fold increase in infectivity half-life over the wild-type for this widely used gene …
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Molekulare Analyse der Penetration von Foamyviren und Konstruktion und Charakterisierung von Adenovirus-Foamyvirus Hybridvektoren
… bei Vesikuläres Stomatitis Virus Glykoprotein (VSV-G) Pseudotypen wurde die FV Env vermittelte Infektion von fast allen Agen-zien, die den endosomalen pH anheben, inhibiert. Allerdings hatte Chloroquin keine inhibitorische Wirkung auf die FV Env vermittelte Infektion im Gegensatz zu der VSV-G …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… calcium also results in major kinetic defects of VSV-G transport to the Golgi, as well as reconstitution of the Golgi after it has been dispersed using brefeldin A. These results suggest that luminal calcium regulates fusogenicity, structure, and function of COPII derived membranes in transport. …
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Luminal calcium regulates membrane fusion in the early secretory pathway
… calcium also results in major kinetic defects of VSV-G transport to the Golgi, as well as reconstitution of the Golgi after it has been dispersed using brefeldin A. These results suggest that luminal calcium regulates fusogenicity, structure, and function of COPII derived membranes in transport. …
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Contribution of the Membrane-Proximal Region of the Vesicular Stomatitis Virus Glycoprotein to Host Cell Entry and Membrane Fusion
<p><em>Vesicular stomatitis virus</em> (VSV) is an enveloped, nonsegmented, negative-sense, RNA virus belonging to the <em>Rhabdoviridae </em>family. VSV is considered the prototypic <em>Rhabdovirus</em> due to its simple genetic organization, broad host cell tropism, and ability to be easily grown …
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Desarrollo de sistema lentiviral para la codificación de un short hairpin RNA que permite el silenciamiento dual de las oncoproteínas E6 y E7 de HPV16 en células de pulmón humano
… células 293T con los vectores AR8.1, VSV-g y el vector recombinante pLKO.1-shRNA (1-4), y se recuperaron vectores lentivirales capaces de transducir células A549pLXSNE6E7. Las células transducidas A549pLXSNE6E7 pLKO.1-shRNA (1-4) obtenidas, fueron utilizadas para evaluar los niveles de …
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Development of a Lentivirus-mediated Gene Therapy Targeting HIV-1 RNA to Eliminate Infected Cells
… I first optimized production of a panel of VSV-G-pseudotyped D4-targeting lentivectors for high infectious titre and low transfer plasmid carryover. I confirmed trans-splicing between HIV-1 *tat1* and *HSVtk*<sub>ΔAUG1</sub> RNA in Jurkat T cells co-transduced with HIV-1<sub>NL4-3ΔE</sub> …