Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"VP16"”.
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Regulation der RNA-Polymerase II Transkription durch humanen Mediator
… die Wirkung des Herpes simplex Virus Aktivators VP16, indem es sowohl an VP16 als auch an Mediator bindet und dessen Aktivitaet reguliert, was letztlich zu einer drastischen Stimulation der RNA-Polymerase II-Transkription fuehrt.
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Targeting a Transcriptional Activator Induces Changes in Large-Scale Chromatin Structure and Nuclear Positioning
… the very strong viral transcriptional activator, VP16, to specific chromosome sites. The rationale of this approach is to use this exaggerated system to amplify effects on large-scale chromatin to the extent where they could be easily analyzed. We have demonstrated a re-programming of the …
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Der bZIP-Transkriptionsfaktor BZI-1 aus Nicotiana tabacum: Analyse der in vivo Funktion durch Modulation der BZI-1- Aktivierungseigenschaften in transgenen Pflanzen
… Expression in BZI-1-DN-Pflanzen reduziert und in VP16-BZI-1-Pflanzen, die ein Fusionsgen aus der VP16-Aktivierungsdomäne und BZI-1 exprimieren, in Abhängigkeit von Auxin verstärkt wird.
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Untersuchungen zur Identifikation von Interaktionspartnern der Nemo-like-Kinase, Nlk
… sowie bekannte Interaktionspartner als VP16-Fusionsproteine exprimiert. Anhand von Reportergenassays konnte gezeigt werden, dass die Co-Expression von Gal-Nlk mit jedem der VP16-Fusionsproteine in einer geringen Transkriptionsaktivierung resultiert. Dies deutet darauf hin, dass Nlk außer …
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Analyse der in-vivo Funktion der Transkriptionsfaktoren TGA2.1 und TGA2.2 aus Tabak nach Fusion mit einer konstitutiven Aktivierungsdomäne
… Aktivierungsdomäne des viralen Proteins 16 (VP16) aus Herpes simplex und den Transkriptionsfaktoren TGA2.1 bzw. TGA2.2 in transgenen Pflanzen überexprimiert werden. Da diese Aktivierungsdomäne einen möglichen SA-regulierten Transaktivierungsschritt ersetzt, können durch die Analyse der …
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Multi-modal imaging of herpesvirus infection
… insight into the roles of pUL16, pUL21, pUL34, VP16, and pUS3 in HSV-1 nuclear egress, and the roles of pUL11, VP16, pUL51, gK, and gE in cytoplasmic envelopment. Furthermore, this analysis indicates that envelopment occurs by budding of tegument-associated capsids into glycoprotein-containing …
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Proteasome Inhibition Enhances The Effect of Bikdd Gene Therapy
… By constructing into a highly amplified “VISA” (VP16-GAL4-WPRE integrated systemic amplifier) system under the control of a cancer specific promoter, previous studies have verified the specific killing effect of BikDD in cancer cells both in <em>in vitro</em> and <em>in vivo</em> models. …
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Improvement and establishment of the tTA-dependent inducible system in the mouse brain
… and toxicity of the transactivation domain VP16. These sequence changes on tTA resulted in the three-fold increase of the inducible activity in transiently transfected HeLa cells, compared to the prokaryotic one. Activity of the htTA was efficiently suppressed by addition of doxycycline. To …
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Molekulare Mechanismen der transkriptionellen Aktivierung durch die Transkriptionsfaktoren c-Jun und v-Jun
… Gegensatz zu den sauren Transkriptionsfaktoren VP16, GCN4 und Hap4, in S.cerevisiae unabhängig vom SAGA- und Ada-Komplex ist. Weder die Funktion des SAGA-Komplexes Histone zu acetylieren, noch seine Funktion als Brückenprotein sind für die transkriptionelle Aktivierung durch v-Jun notwendig. …
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SWI/SNF-NUCLEOSOME INTERACTIONS AND DISASSEMBLY OF NUCLEOSOMES: NOVEL METHODOLOGIES FOR MAPPING PROTEIN-PROTEIN AND PROTEIN-DNA INTERACTION
… eviction and the recruitment of SWI/SNF by Gal4-VP16 to dinucleosomes restricted the nucleosome mobilization in one direction. Finally, based on the data from this study and previous reports, the mechanism how recruitment and neighboring nucleosomes alter the outcome of SWI/SNF remodeling is …
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Experience-Dependent and Input-Specific Regulation of Neocortical Circuit Development by Genes Linked to Neurodevelopmental Disorders
… a transcriptionally active form of MEF2C (MEF2-VP16), suggesting that MEF2C transcriptional activation drives experience-dependent development of L4-L2/3 synapses. Together, my findings on Fmr1 and Mef2c demonstrate an interaction of experience and gene functions in regulation of specific …
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The Design and Development of Artificial Zinc Finger Transcription Factors and Zinc Finger Nucleases to the hTERT Locus
… and made ZFTFs by linking the ZFPs to the VP16 transcriptional activation domain. We were able to identify several active ZFTFs that demonstrate a dose-dependent response. The same ZFPs were also converted into ZFNs and screened in combinatorial pairs in cell-based single-strand annealing …
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Reengineering Estrogen Receptor Hormone Specificity Through Coordinated Changes at Key Contact Sites Between Estrogen Receptor and Estradiol
… or E353A mutant ER and the activation domain of VP16. These chimeric proteins were tested in mammalian cell transfection assays, and although these chimeric receptors required higher levels of ligand than the full length receptor, the specificity shifts mirrored those we had seen previously. …
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ANALYSIS OF HERPES SIMPLEX VIRUS ICP0 FUNCTION AND THE ANTIBODY RESPONSE TO A LIVE HSV-2 ICP0- MUTANT VACCINE
… a Tet-regulatable gene-of-interest driven by the VP16- inducible HSV-1 ICP0 promoter and 2. a highly expressed bicistronic gene that expresses the Tet-repressor protein and a puromycin selection marker. The modified bidirectional HSV-1 ICP0 promoter used in this novel expression system 1. drives …
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Differential regulation of herpes simplex virus-1 and herpes simplex virus-2 during latency and post reactivation in response to stress hormones and nerve trauma in primary adult sensory and sympathetic neurons
… (IE) genes ICP0, ICP4, ICP27 and trans-activator VP16 following treatment with stress hormones and NTF deprivation in HSV-1 and HSV-2 infected neurons. We also show that different molecular mechanisms are involved in HSV latency and reactivation, which are dependent on the stimuli and the type of …
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Analysis of gamma secretase regulation by phosphatidylethanolamine using mammalian and Drosophila melanogaster in vitro and in vivo model systems
… human APP-C-terminal fragment, fused to the GAL4-VP16 (GV) transcription factor. Membrane PE levels in these ??secretase reporter flies were depleted by introducing the easPC80 mutation, that affects ethanolamine kinase (ETNK) an enzyme involved in PE synthesis pathway. A strong downregulation of …