Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"VIGS"”.
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Die keuse van babavoedingswyse binne die MIV/VIGS-epidemie : voorligtingsimplikasies
Approximately one third of the women who visit the antenatal clinics in the North West Province are HIV positive. These women need to decide on the most appropriate method of feeding for their babies. As early as 1985 it was proven that HIV transmission through breast milk could take place. …
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Emosionele behoeftes van die MIV/VIGS geaffekteerde Kleurlingkind in die middelkinderjare (Afrikaans)
The study is aimed at looking at the emotional needs the HIV/Aids affected coloured child in middle childhood. An in depth literature study was done and shortcomings have been identified with regard to the HIV/Aids affected child, in specific the experience of the child within the period before the …
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Gene discovery in Catharanthus roseus using virus induced gene silencing
… the use of Virus Induced Gene Silencing (VIGS) for the discovery of enzymes and transporters involved in monoterpene indole alkaloid (MIA) metabolism in the medicinal plant Catharanthus roseus. C. roseus is the source of a number of MIAs that are used as chemotherapeutic agents in the …
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Role of virus genes in seed and aphid transmission and development of a virus-induced gene silencing system to study seed development in soybean
… of a DNA-based virus induced gene silencing (VIGS) vector for investigating genes expressed in the soybean seed. VIGS are reverse genetics tools comprised of virus vectors with a partial plant transcript insertion that can silence expression of the plant gene. TSV has a tripartite genome, and …
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Dissecting key biomass traits through traditional QTL mapping, exploring novel functional genomics approaches, and investigating self-incompatibility in Miscanthus
… imperfect. Virus induced gene silencing (VIGS) may present an ideal alternative. This technique is rapid and robust to polyploidy, but it is untested in Miscanthus. Here we attempted to adapt an existing VIGS vector with a large monocotyledonous host range for use in Miscanthus. Barley …
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Functional Characterization of Monoterpenoid Indole Alkaloid (MIA) Biosynthetic Genes in Catharanthus roseus
… genes. Virus-induced gene silencing (VIGS) is being used to screen these candidate genes for their involvement in iridoid biosynthesis pathway, especially in the identification of 7-deoxyloganic acid 7-hydroxylase (CrDL7H) shown by the accumulation of its substrate, 7-deoxyloganic acid …
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Expanding the Role of Oxidoreductases in Benzylisoquinoline Alkaloid Metabolism in Opium Poppy
… derivatives. Virus-induced gene silencing (VIGS) used to suppress the abundance of CODM and/or T6ODM transcripts indicated a direct physiological role for these enzymes in the metabolism of protopine alkaloids, and revealed their indirect involvement in the formation of the antimicrobial …
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Advanced applications of Raman spectroscopy and super-resolution imaging of biological and plant materials
… changes from virus-induced gene silencing (VIGS). VIGS is a powerful technique to study gene function, but few tools can study the spatial dependence of its effects at the cellular level. The combined imaging methods uncovered the spatial distribution of the effects from silencing the …
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The Evolution and Development of Nectar Spurs
… tobacco; through virus-induced gene silencing (VIGS) of candidates in *L. vulgaris*; and through the attempted overexpression of one candidate in *L. vulgaris*. Protocols for VIGS and stable transformation were trialled and developed for use in *L. vulgaris*, marking the first successful …
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Investigating the role of neuropeptides in plant parasitic nematodes
… Additionally, a Virus Induced Gene Silencing (VIGS) method was used to try and silence nlp-15 in invading Meloidogyne incognita nematodes. Taken together, the data highlight a need to further understand gene silencing techniques with respect to neuronal targets so we can develop methods that …
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Functional Characterization of Metallothioneins in Arabidopsis and Barley
… metal homeostasis. Virus induced gene silencing (VIGS) was used to study the function of <em>MT1a</em> in barley; however, the virus could not silence the expression of this gene. Further investigation on the stability of the MT RNA fragment in the VIGS virus will be necessary.</p> <p>Taken …
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The γ-tocopherol-like family of N-methyltransferases: A taxonomically clustered gene family encoding enzymes responsible for N-methylation of monoterpene indole alkaloids
… and Virus Induced Gene Silencing (VIGS) suppression of the messenger has since proven its involvement in vindoline biosynthesis. Recent large scale sequencing initiatives, performed on non-model medicinal plant transcriptomes, has permitted identification of candidate genes, …
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Involvement of Translationally controlled tumor protein in Tomato yellow spot virus infection
… benthamiana plants silenced for TCTP by VIGS experiments were used to study the effect of TCTP expression on infection by the begomovirus Tomato yellow spot virus (ToYSV). TCTP silencing led to higher accumulation of the virus, suggesting that TCTP is a host factor involved in viral …
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Sequence-specific gene silencing by transitive signals in plants
… some characteristics of transitive silencing. VIGS has been shown to spread over a distance of at least 1000 nt from the 5’ end to the 3’ end of the target mRNA, while 3’ to 5’ spreading can extend at least through 332 nt, with a possible limit of 600 nt (Petersen and Albrechtsen, 2005; Vaistij …
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An investigation into the role of ubiquitination in plant immunity
… Moreover, virus induced gene silencing (VIGS) of NbPOB1 accelerated the dark-induced senescence in N. benthamiana leaves. These observations identify NbPOB1 and NtPOB1 as the orthologs of AtPOB1. The subcellular localization of AtPOB1 and NbPOB1 was analyzed by transiently overexpressing …
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Identication of Candidate Resistance Genes against Rhynchosporium in Barley
… with R. commune. Virus induced gene silencing (VIGS) was carried out in an attempt to validate the Rrs1 candidate. Tightly linked molecular markers for Rrs1 and Rrs18 will provide a useful tool for breeders aiding marker assisted selection.
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An investigation of gene expression, alternative splicing and nonsense-mediated decay in potato in response to Phytophthora infestans infection
… plants using virus induced gene silencing (VIGS), I explored the role of NMD in R/Avr interactions and disease development and found a delayed defence response. Finally, I exploited a recently developed high-quality Reference Transcriptome Dataset for potato (StRTD) to analyse RNA-seq data …
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An investigation into the role of deubiquitinating enzymes in plant disease resistance
… ortholog NtUBP12 was determined and utilised for VIGS based NbUBP12 gene silencing studies during disease resistance signalling in N. benthamiana. Loss of function studies indicated that NbUBP12 functions as a negative regulator of hypersensitive cell death (HR) induced by the Cladisporium fulvum …
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The use of reverse genetics to investigate plant-parasite interactions
This thesis demonstrates the development and use of a novel Virus Induced Gene Silencing platform to investigate the interactions of plants and parasites. Host and parasite genes are targeted using this technology which when silenced indicate their involvement in establishing pathogenicity. The …
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Persistent viruses in Capsicum annuum plants: Understanding the mystery guests
Most work on plant viruses focuses on their pathogenicity, but recent studies suggest viruses may benefit their hosts, at least under some conditions. Plant-infecting viruses of the Partitiviridae and Endornaviridae are transmitted only vertically, via pollen and seed, generally cause no disease …