Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"VHH"”.
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Developing VHH-based tools to study Ebolavirus infection
… camelid-derived heavy chain-only antibodies, or VHHs, have emerged as a unique antigen binding moiety that holds promise in its versatility and utilization as a tool to study biological questions. This thesis focuses on two aspects on developing tools to study infectious disease, specifically …
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Development of VHH- and antibody- based imaging and diagnostic tools
… a controlled manner. Single-domain antibodies (VHHs) from camelids are amongst the smallest (15 KDa) proteins that can recognize a diverse set of targets with excellent specificity. Chemoenzymatic labeling of molecules using sortase allows site-specific attachment of a single label of interest …
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Engineering VHH-based chimeric antigen receptor (CAR) T cell therapy for solid tumor treatment
… The use of single domain antibody (VHH)-based CAR T cells that recognize these markers circumvents the need for tumor-specific targets. Chapter 3 will describe methods to overcome the immunosuppressive microenvironment of solid tumors. Here, we have developed VHH-secreting CAR T …
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Characterisation of llama antibody fragments able to act as HIV-1 entry inhibitors
… properties confined to a single fragment, the VHH, and a preference for cleftrecognition. VHH were isolated from llamas immunised with recombinant gp120 using phage display-based methods. In order increase the chances of isolating neutralising VHH, a functional selection strategy was employed, …
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Phonetic Grounding and Phonology: Vowel Backness Harmony and Vowel Height Harmony
… conclude that the difference between VBH and VHH indicates that phonological approaches that model vowel harmony as spreading or agreement of features are inadequate. Within those models, there is no prediction that VBH and VHH should be fundamentally different. Typological patterns of harmony …
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Development of potential immunodiagnostic & therapeutic techniques using SNAP-fusion proteins as tools for the validation of Triple-negative Breast Cancer
… fragment (scFv) & single domain/nanobody (VHH) antibody formats for diagnostic and therapeutic purposes. In this work, these recombinant antibody fragments have been combined with SNAP-tag, a modified version of the human DNA repair enzyme O6-alkylguanine-DNA-alkyltransferase (AGT), which …
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Holdafar, hreyfing og heilsutengd lífsgæði eldri aldurshópa
… þjálfunarhópa (ÞHH og ÞHÁ) og viðmiðunarhóps (VHH) og milli kynja. Þá voru athugaðar nokkrar valdar breytur úr holdafarsmælingum (hæð, þyngd, fituprósentu (DEXA) og mittis- og mjaðmamáli) og sex mínútna gönguprófi. Heildarfjöldi þátttakenda var 173 en 146 einstaklingar kláruðu 26 vikna þol- og …
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Generating single-domain antibodies against fibronectin splice variants
… these libraries to pan against, I selected for VHH which bind to EIIIA and EIIIB. Since these splice variants are upregulated in tumor angiogenesis and are rarely seen elsewhere in adult tissues, antibodies targeting EIIIA or EIIIB may be of use for imaging tumors and metastases.
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Transcriptomics-Guided Biodiscovery of Peroxidases from Macroalgae
… coli as a fusion with a single-domain antibody (VHH). The fusion protein was successfully applied as a reporter enzyme in an ELISA, demonstrating both target binding and a detectable signal. Overall, this work establishes a transcriptome-based approach for enzyme discovery in macroalgae and …
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Developing a novel hepatitis B core – based antigen presentation system
… fragments of camelid origin (nanobodies, or VHH). The resulting “tandibody” particles, as they are named here, can bind to their cognate antigen to yield CLPs covered in the antigen of interest. Furthermore, it was shown that target antigens can be attached to CLPs via a fusion partner, …
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Development of SNAP-tag-based fusion proteins targeting HIV-1 viral reservoirs
… surface of tumour cells. The HIV neutralizing VHH-nanobody J3, isolated from an immunised Llama has demonstrated anti-HIV properties against more than 95 % of HIV strains in vitro. As part of an ongoing project to develop a J3-ETA IT, this work sought to produce a J3-SNAP fusion protein by …
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Using data to characterise the relationship between nanobody sequence, structure and function
Nanobodies (Nbs) are a class of single-domain antibody derived from the immune systems of camelid species. They achieve binding affinities and specificities to target antigens comparable to those of classical antibodies (Abs), despite being ten times smaller (~15 kDa) and having only three variable …
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Studies of Protein Stability by Hydrogen Exchange-Mass Spectrometry
… we expressed and purified a set of mutants of VHH-F5 and a binding assay was optimized to identify the mutants with differences in relative binding affinities. The mutants with detuned binding affinities will be used to perform HX-MS experiments to identify their higher-order structural …
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Isolation and Characterization of Anti-SLP Single Domain Antibodies for the Therapy of C. difficile Infection
Clostridium difficile is the leading cause of death from gastrointestinal infections in Canada. Current antiobiotic treatment is non-ideal due to the high incidence of relapse and the rise in hyper-virulent antibiotic-resistant strains. Surface layer proteins (SLPs) cover the entire bacterial …