Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"VGLUT1"”.
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PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1)
The vesicular glutamate transporter 1 (VGLUT1) is an important membrane protein located in glutamatergic synaptic vesicles. It is responsible for the storage and release of the excitatory neurotransmitter glutamate. VGLUT1 is a highly hydrophobic integral membrane protein with a molecular weight …
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PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1)
The vesicular glutamate transporter 1 (VGLUT1) is an important membrane protein located in glutamatergic synaptic vesicles. It is responsible for the storage and release of the excitatory neurotransmitter glutamate. VGLUT1 is a highly hydrophobic integral membrane protein with a molecular weight …
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The vesicular glutamate transporter (VGLUT): heterologous expression, proteoliposome, computational and mass spectral studies
… to characterize them. Plasmid DNA encoding rat VGLUT1 was constructed, amplified and expressed in Pichia pastoris to produce VGLUT1 protein. Immobilized metal affinity chromatography (IMAC) was employed to purify the protein for structural analysis by mass spectrometry and to develop a …
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The vesicular glutamate transporter (VGLUT): heterologous expression, proteoliposome, computational and mass spectral studies
… to characterize them. Plasmid DNA encoding rat VGLUT1 was constructed, amplified and expressed in Pichia pastoris to produce VGLUT1 protein. Immobilized metal affinity chromatography (IMAC) was employed to purify the protein for structural analysis by mass spectrometry and to develop a …
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On the chloride dependence of vesicular glutamate transport
… von Mäusen untersucht, bei denen entweder das VGLUT1-Gen deletiert war oder aber das Gen für einen mutmaßlichen Chloridtransporter auf synaptischen Vesikeln - ClC-3. Hierbei ergaben sich klare Indizien dafür daß Chloridionen nicht durch ClC-3 ins Vesikellumen gelangen, sondern durch VGLUT1 …
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Effects of Neuronal Nogo-A on Properties of Excitatory Synapses of the Sensorimotor Cortex
… We then used vesicular glutamate transporter 1 (vGlut1) as a marker for potential excitatory synapses. Knocking down Nogo-A in postnatal pyramidal neurons of the sensorimotor cortex led to a decrease in the number of vGlut1 puncta identifying a potential presynaptic partner, in opposition to the …
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Spinal interneurons in sensorimotor integration
… for the vesicular glutamate transporter 1 (VGLUT1) but those cells from the second group received few contacts of this type and were predominantly contacted by terminals immunoreactive for vesicular glutamate transporter 2 (VGLUT2). This result was as predicted because VGLUT1 is found …
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CHRONIC LOW-LEVEL LEAD EXPOSURE AFFECTS THE MONOAMINERGIC SYSTEM IN THE MOUSE SUPERIOR OLIVARY COMPLEX
… sections were immunolabeled with antibodies to VGLUT1, VGAT and VAChT in order to determine whether Pb exposure alters the glutaminergic, gaba-ergic, or cholinergic systems. Pb exposure caused a significant decrease in VMAT2, 5HT, and DβH expression while VGLUT1, VGAT and VAChT showed no change. …
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CHRONIC LOW-LEVEL LEAD EXPOSURE AFFECTS THE MONOAMINERGIC SYSTEM IN THE MOUSE SUPERIOR OLIVARY COMPLEX
… sections were immunolabeled with antibodies to VGLUT1, VGAT and VAChT in order to determine whether Pb exposure alters the glutaminergic, gaba-ergic, or cholinergic systems. Pb exposure caused a significant decrease in VMAT2, 5HT, and DβH expression while VGLUT1, VGAT and VAChT showed no change. …
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Ossitocina nella Sostanza Nera del ratto: azione sull'attività locomotoria e interazione con i neuroni dopaminergici, glutammatergici e GABAergici nigrali
… against TH, vesicular glutamate transporters (VGluT1, VGluT2 and VGluT3) and glutamate decarboxylase (GAD). Together, the results of the above experiments with oxytocin and d(CH2)5Tyr(Me)2-Orn8-vasotocin and those with OXY-SAP, which revealed the existence of a correlation between the changes …
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Characterizing an AraC-Differentiated, Heterogeneous NT2-Derived Population
… the NT2N cells consisted of glutamatergic (anti-VGluT1) and GABAergic (anti-GAD67) subtypes in a 4.8:1 ratio, respectively, which is similar to the ratio found in vivo. Preliminary functional studies performed via electrophysiology indicate that these cells possess the machinery for spontaneous …
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The neural cell adhesion molecule 2 (NCAM2) promotes excitatory synapse formation through increasing insulin and insulin-like growth factor 1 receptor signaling
… developmental increase in synaptophysin and VGLUT1 levels but does not affect the developmental increase in VGAT levels. Synapse densities are reduced in NCAM2-deficient cultured hippocampal neurons when compared to NCAM2-expressing neurons. In NCAM2-expressing neurons, inhibition of insulin …
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Biochemistry and physiological role of otoferlin
… an den vesikulären Glutamat-Transporter 1 (VGLUT1). PHluorin ist eine pH-Wert abhängige GFP-Variante, die bereits in Neuronen verwendet wird. Seine Fluoreszenz erhöht sich bei der Vesikel Fusion mit der Plasmamembran und wird gequencht beim Ansäuern des vesikulären Lumens nach Endozytose, …
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Direct generation of region-specific induced neural Precursors from adult human fibroblasts using non-viral and viral methods
… cells was assessed using neuronal markers NSE, VGLUT1, and GAD65/67 after differentiating in the neuronal media. Lentiviral-derived populations were further enriched by FACS, and NSE-positive neurons comprising either glutamatergic or GABAergic phenotypes were efficiently generated from …
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ROLE OF ATM IN THE PATHOPHYSIOLOGY OF MAJOR DEPRESSIVE DISORDER (MDD)
… by the reduction of mEPSCs frequency and of VGLUT1 expression – after 1 hr, but not after 24 hr indicating an acute effect of factors release by astrocytes. Remarkably, neurons treated with the ACM of astrocytes where Atm mRNA was silenced via siRNA, exhibited the same functional defects, …
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Novel Optical Tools to Investigate Presenilin Control of Neurotransmission
… in regulating neurotransmission. We utilized vGlut1-pHluorin, a pH sensitive analog of GFP tagged to a synaptic vesicle protein, that allows for reporting of synaptic vesicle cycling. These experiments found evidence that Presenilins do control neurotransmission and that they do so by …
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Synaptic vesicle protein 2A-dependent function and dysfunction at the presynapse
… vesicular glutamate transporter 1 (VGLUT1) and SYT1 recycling were observed, suggesting that LEV is unlikely to function as a modulator of excitatory presynaptic activity or by influencing SV2A function. In conclusion, this work demonstrates that SV2A is essential for accurate SYT1 …
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An omics study into the molecular impact of autosomal dominant APP and MAPT mutations on the cerebral cortex
… cholesterol synthesis upregulation in VGLUT1+ excitatory neurons carrying MAPT E10+16 mutation using an independent organoid-derived single-cell RNA sequencing dataset (Bertucci et al., 2023). Small RNA sequencing from bulk cultures carrying MAPT E10+16 mutation points out …
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CXCL12/CXCR4 signaling in mesocorticolimbic reward pathways: relevance to cocaine reinforcement and relapse
… for vesicular glutamate transporter 1 (vGlut1) and glial fibrillary acidic protein (GFAP) showing that the glutamatergic neurons and astrocytes in the PFC express CXCR4. To assess the effects of CXCL12 on GLU transmission, microdialysis of NAc was performed. Following unilateral …