Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 39 for “"VEGFR2"”.
-
The Immunosuppressive Function of VEGF Signaling in the Tumor Microenvironment
… We and others have shown selective elevation of VEGFR2 expression on tumor-associated myeloid cells in tumor-bearing animals. I further investigate the function of VEGFR2+ myeloid cells in regulating tumor immunity and find VEGF induces an immunosuppressive phenotype in VEGFR2+ myeloid cells …
-
Chronic Hypoxia and Cardiovascular Dysfunction in Sleep Apnea Syndrome
… vascular endothelial growth factor receptor-2 (VEGFR2) gene expression by redundant oligonucleotide DNA microarray and real-time PCR technologies. Circulating angiogenic progenitor cells expressing VEGFR2 were profiled by flow cytometry. Plasma and serum concentrations of VEGF, …
-
Multiplexed angiogenic biomarker quantification on single cells
… having high concentrations of membrane VEGFR1, VEGFR2, EGFR, IGFR, and PDGFRs. To gain greater insights into cell heterogeneity and examine angiogenic signaling pathways as a whole, I utilize the unique spectral properties of quantum dots (Qdots), and combines Qdots with qFlow cytometry, to …
-
Elucidating how αvβ3-integrin regulates neuropilin-1’s role in tumour angiogenesis in order to improve anti-angiogenic therapy
… occurs via a compensatory upregulation of VEGFR2 and enhancement in VEGFR2-neuropilin-1 interactions. Here, I show that tumour growth and angiogenesis are sensitive to neuropilin-1 perturbation even with only a 50% reduction in β3-integrin expression. β3-integrin-heterozygous, but not …
-
Discovering angiogenic cell signaling landscapes: Quantitative mapping through experiment and computation
… interactions—primarily via VEGF-A:VEGFR2 binding. But targeting the VEGF family alone has not achieved the promise of stable vascular control, because angiogenesis involves other signaling axes, such as the PDGFs. In cancer, for example, anti- angiogenic therapeutics primarily …
-
Directed Migration Achieved in Mammalian Cells via Ca2+ Signal Rewiring in Synthetic Protein Chimeras
… motility and exocytosis. In some receptors (e.g. VEGFR2), Ca2+ signals are generated upon ligand binding (e.g. VEGF-A). Here, firstly we engineered fusion proteins that generate a Ca2+ signal upon ligand binding by creating fusions of domains that oligomerize to the transmembrane domain and the …
-
An Optogenetic Toolbox for the Interrogation of Dynamic Events During Angiogenesis
… tip cell development, we designed an optogenetic VEGFR2 utilizing the LightR regulatory system. We demonstrated that LightR regulation of RTKs provides spatiotemporal control over VEGFR2 signaling. Furthermore, we demonstrated that previous VVD modifications conserved their effect in RTK …
-
Matrix Metalloproteinases Regulate The Differentiation Of Adipose-Derived Mesenchymal Stem Cells Into Endothelial Cells
… mechanism of this effect, immunostaining of VEGFR2 was performed with and without MMP-2 and MMP-14 siRNAs. Findings revealed that MMP-2 and MMP-14 knockdown decreased the cleavage of VEGFR2, and inhibition of VEGFR2 significantly decreased the expression of EC markers. However, siRNA …
-
Bioactivity and Cell-Mediated Targeting of Multistage Nanoporous Silicon Particles
… vascular endothelial growth factor receptor 2 (VEGFR2) to enhance vascular targeting and relying on the natural tumor tropism of mesenchymal stem cells (MSC) to achieve dynamic targeting were investigated. Briefly, successful modulation of the release kinetics was achieved by adjusting the pore …
-
Coordinate regulation of Vegf signalling genes by the proline rich homeodomain protein
… via cell surface receptors including VEGFR1 and VEGFR2. This thesis identifies Vegf, Vegfr1, and Vegfr2 as bona fide PRH target genes and shows PRH can control cell survival through the modulation of VEGF and VEGF receptor signalling. CK2 is a stress-activated protein kinase with pleiotropic …
-
Part 1: Isolation of Orexin Receptor Regulators via a Microarray-Based,Two-Color Cell Binding Screen. Part II: Targeted Inactivation of Proteins triggered by Visible Light.
… conjugated to small molecule ligands targeting VEGFR2 or the 26S proteasome. When irradiated with visible light, these reagents showed significantly increased potencies in inhibiting VEGF-induced VEGFR2 activation or proteolytic activity of the 26S proteasome.
-
Anti-VEGF Therapy Modulates Immune Cell Infiltration and Function in Multiple Breast Cancer Models
… activates two primary VEGF receptors, VEGFR1 and VEGFR2. VEGFR2 is the primary angiogenic receptor, while the function of VEGFR1 is less defined. It is important to note that the VEGFRs are expressed on endothelial cells, tumor cells and on many host immune cells. Therefore, to better understand …
-
Investigating the Impact of Environmental Stressors on Skeletal Muscle Angio-Adaptation
… endothelial cells proliferation and attenuated VEGFR2 activation. Endothelial cells pre-conditioned to cold-stress and challenged for migration in re-warming conditions however, exhibited an enhanced migratory responsiveness.
-
Elucidating the role of Gremlin1 in colorectal cancer
… non-canonical signalling mechanism of GREM1 is VEGFR2 activation. Despite exhaustive efforts in a multitude of cell lines, we could not demonstrate GREM1-mediated VEGFR2 activation. Computational genomics was used to compare gene expression changes in the intestines of wild-type versus …
-
THE IMPACT OF VASCULAR SMOOTH MUSCLE CELL SENESCENCE ON MITOCHONDRIA DYSFUNCTION AND ANGIOGENESIS
… di specifiche vie correlate al SASP (VEGF/VEGFR2 e NF-κB) possa rappresentare strategie terapeutiche più efficaci. RISULTATI: Abbiamo dimostrato, utilizzando sia datasets di sequenziamento dell’RNA a singola cellula sia l’immunoistochimica, che le VSMC senescenti sono significativamente …
-
Discovery of Pyrimidine-based Heterocycles as Single Agents With Combination Chemotherapy Potential And As Inhibitors Of Purine Nucleotide Biosynthesis For The Treatment of Cancer
… vascular endothelial growth factor receptor-2 (VEGFR2), platelet derived growth factor receptor-β (PDGFRβ) and epidermal growth factor receptor (EGFR) in single entities. Single agents with both antiangiogenic activities as well as cytotoxicity would afford agents that circumvent pharmacokinetic …
-
Dynamics of vascular normalization during anti-angiogenic therapy : implications for combination therapy
… Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), prunes immature blood vessels, reduces vascular diameter and improves pericyte and basement membrane coverages. Functionally, the vascular permeability to macromolecules and interstitial fluid pressure are reduced. By lowering interstitial …
-
The Influence of Obstructive Sleep Apnea Syndrome on Insulin Resistance, Metabolic Syndrome, and Endothelial Dysfunction in Young Men
… and endothelial dysfunction (CRP, VEGF, and VEGFR2) using ELISA, RIA, and flow cytometry. MetS was defined according to Adult Treatment Panel III (ATP III) clinical standards. Triglycerides, HDL cholesterol, and glucose were measured using a commercial lipid panel. Resting blood pressure was …
-
Adipogenesis and angiogenesis : roles in tissue engineering and glucose metabolism
… are mutually dependent via a VEGF receptor 2 (VEGFR2)-mediated mechanism. Since then several other studies have reinforced a role for the VEGF-VEGFR system in energy metabolism. For example, genetically obese mice treated with anti-VEGF antibody had lower fat pad weights, but the VEGF receptor …
-
Invasion and Proliferation in Malignant Cells
… primary breast cancer, a signaling cascade from VEGFR2 via ERK1/2 phosphorylation to Ets2 phosphorylation and cyclin D1, could be outlined and ERK1/2 phosphorylation was linked to small tumors with good prognosis. We also observed a Notch1 independent activation of Hes1 in breast cancer. Further, …
Page 1 of 2