Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"VDJ"”.
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MULTIPLE MYELOMA PLASMA CELL PROFILING: INTEGRATED SINGLE-CELL RNA AND VDJ SEQUENCING APPROACHES UNCOVER NOVEL CLINICAL INSIGHTS
… del sequenziamento a singola cellula di RNA e VDJ (scRNAseq e scVDJseq) per esplorare in dettaglio i paesaggi genetici e trascrittomici del MM a livello di singola cellula, con particolare enfasi sulle pPCs, sulle interazioni con il TME e sull’evoluzione clonale. Il primo risultato principale …
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ANALYSIS OF B LYMPHOCYTES IN MOUSE MODEL LIGASE IV WITH HYPOMORPHIC MUTATION IN VDJ RECOMBINATION ASSOCIATED WITH GROWTH DEFECT
The major mechanism for the repair of DNA doublestrand breaks (DSBs) in mammalian cells is non-homologous end-joining (NHEJ), a process that involves the DNA-dependent protein kinase , XRCC4 and DNA ligase IV. Rodent cells and mice defective in these components are radiation-sensitive and defective …
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Regulation Of Selection And Central Tolerance By The N-Terminal Region Of Rag1
… rearranges antigen receptor gene segments during VDJ recombination to allow for the generation of a vast array of antigen receptors with specificities against a virtually infinite number of insults. Direct consequences of VDJ recombination include the formation of non-functional receptors, and …
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Investigation of IGF1R/IRS1 Signalling Mechanisms in B Cell Development
… (J) genes are differentially rearranged in VDJ recombination. This assembles a complete B cell receptor (BCR) and is the primary step in producing a diverse BCR repertoire; an essential requirement for a robust adaptive immune response. The roles of interleukin-7 receptor, pre-BCR and …
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STUDIES OF B CELL DEVELOPMENT AND V(D)J RECOMBINATION
… unbiased repertoire quantification technique, VDJ-seq, allowed me to study recombination of the human IGH locus sequence and observe HCAb repertoire selection within the mouse environment. The adaptation of next generation sequencing techniques to antigen receptor repertoire quantification has …
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Untersuchung der Entwicklung der T-Zell-Rezeptor assoziierten Signalleitung im Thymus auf Einzelzellniveau
… die Expression der produktiv umgelagerten TZRß VDJ Gene die erste zelluläre Antwort ist, die von der CD3-Signalleitung abhängig ist, während die TZRß-Genumlagerung unabhängig davon stattfindet (A. Würch et al. J. Exp. Med. 188, 1669, 1998; J. Biro et al. Proc. Natl. Acad. Sci. 96, 3882, 1999). …
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Mechanism for Pre-B Cell Loss in Mutant Ali/Ali Rabbits
… ali/ali rabbits predominantly utilize VH4 during VDJ gene rearrangement. However, most of the VH4-utilizing B cells encounter a severe block early in B cell development. In contrast, wildtype rabbit B cells predominantly rearrange VH1, a gene >90% identical to VH4, and develop normally. We …
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The role of DEAD-box helicase 1 in humoral immune responses
… Primary diversification occurs through VDJ recombination during B-cell development, whereas secondary diversification occurs during ongoing immune responses through the introduction of affinity enhancing point mutations in Ig variable genes, a process termed Somatic Hypermutation (SHM). …
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A novel genomics and bioinformatics approach to assess immunoglobulin and T cell receptor rearrangements and somatic hypermutation in lymphoproliferative disorders
… was shown to accurately detect clonal VDJ recombination by targeting one side of the rearrangement and using lower read lengths, down to 75 bp.<br/><br/>NGS SHM analysis using the 1.5 kb genomic sequence from the clonally rearranged joining gene towards the enhancer region (IGHJ-E) was …
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Post Translational Regulation of AID Targeting to Both Strands of a Transcribed DNA Substrate
… hypermutation (SHM). Both reactions occur after VDJ recombination, subsequent to antigen exposure. During CSR, a deletion and recombination event occur to alter the effector function from IgM to either IgG, IgE, or IgA. SHM then occurs, which introduces point mutations at a high frequency into …