Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"VASP"”.
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Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development
… the family consists of Mena (Mammalian Enabled), VASP (Vasodilator Stimulated Phosphoprotein), and EVL (Ena-VASP-Like). This thesis work focused on understanding the vertebrate EnaNASP protein family by discovering pathways that regulate EnaNASP function and by defining the role of EnaNASP …
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A Study of Improving the Parallel Performance of VASP.
… research on molecules. The application, VASP, was migrated from a machine with 4 nodes and 16 single-threaded processors to a machine with 60 nodes and 120 dual-threaded processors. When initially migrated, VASP's performance deteriorated after about 17 processing elements (PEs), due to …
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Ena/VASP proteins regulate vertebrate nervous system development : a thesis presented
… upon regulation of the actin cytoskeleton. Ena/VASP proteins are able to remodel the actin cytoskeleton in response to extracellular signals and have been shown to regulate the motility and morphology of a variety of cells. I have investigated the in vivo requirement for the vertebrate family …
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Die Rolle des Proteins VASP für die Proliferation und Differenzierung hämatopoetischer Stammzellen
… Vasodilatator stimulierenden Phosphoproteins (VASP) untersucht. Es wurde ein neuer Antikörper (5C6) charakterisiert, der für an Serin157 phosphoryliertes VASP spezifisch sein sollte. Es konnte gezeigt werden, dass der 5C6 Antikörper spezifisch VASP erkennt, welches an der Stelle Serin157 …
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Repulsive axonal pathfinding requires the Ena/VASP family of actin regulatory proteins in vertebrates
… deficient for all three vertebrate Ena/VASP homologues: Mena, VASP, and EVL. As has been reported previously in invertebrates, these defects share phenotypic overlap with those seen in mice genetically deficient for the repulsive guidance molecules Slit and Robo. I find that the …
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Analysis of signal transduction pathways and the cytoskeleton in VASP-deficient cell lines and mouse models
… mammalian Vasodilator Stimulated Phosphoprotein (VASP) is a founding member of the Ena/VASP family of proteins that includes Drosophila Enabled (ena), the mammalian Ena homologue (Mena) and the Ena-VASP-like protein (Evl). VASP was initially discovered and characterized as a substrate for cGMP- …
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The role of Ena/VASP and associated proteins in regulation of neuronal morphology and filopodia architecture
… remodeling remain poorly understood. The Ena/VASP family of proteins function as key signaling molecules that influences actin reorganization in response to environmental cues, and has been implicated in many aspects of development. My work has focused on defining the mechanisms by which the …
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Bedeutung des "vasodilator-stimulated phosphoprotein" (VASP) für die Entwicklung des Hörvermögens von Säugetieren - eine experimentelle Studie an Mäusen -
Das „vasodilator-stimulated phosphorprotein“ (VASP), ein Mitglied der Ena/VASP Proteinfamilie, gilt als ein entscheidender Faktor bei der Regulation von Aktindynamik. Es ist an Zellbeweglichkeit und –adhäsion, unter anderem in Filopodien und dem Wachstumskegel auswachsender Neurone beteiligt. …
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Funktionelle Charakterisierung des Vasodilatator stimulierten Phosphoproteins (VASP) für die Stabilität des Aktin-Zytoskeletts und die Integrin-abhängige Zelladhäsion
Das Vasodilatator stimulierte Phosphoprotein (VASP) ist ein Zytoskelett-assoziiertes Protein der Ena (Enabled)/VASP-Proteinfamilie. Seine Funktionen bezüglich Aktin- Polymerisation, Thrombozyten-Aggregation, Wachstumskegel-Führungsprozessen und Motilität sowohl von Zellen als auch von Listerien …
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A lentiviral system for RNAi transgenesis and the Ena/VASP triple-knockout defines neuronal and non-neuronal functions in mouse development
… signaling pathways remains unclear. The Ena/VASP protein family is a common downstream effector of multiple axon guidance signaling cascades. The analysis of the Ena/VASP triple-null mouse allows us to determine the extent to which these intracellular cascades have been conserved in the …
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Force-mediated adhesion strengthening in endothelial cells at adherens junctions
… and vasodilator-stimulated phosphoprotein (VASP) both associate with adherens junctions, so their role in adhesion strengthening at adherens junctions was also studied. Cells treated with actin-inhibitor cytochalasin D showed no difference in bead detachment from constant high force and from …
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How short, degenerate motifs across the human proteome recognize the actin remodeling factor ENAH
… in modulating binding to the SLiM-binding Ena/VASP EVH1 domain family. I identified three mechanisms by which adjacent and distal sequence elements surrounding native EVH1-binding SLiMs enhance binding affinity and specificity to ENAH, an Ena/VASP paralog. I also determined the strategies …
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Toca-1 driven actin polymerisation at membranes
… as the SH3 domain of IRSp53, which binds Ena and VASP). I investigated the contribution of the F-BAR protein Toca-1 in linking actin polymerisation to membranes, by characterising a new protein-protein interaction and the interaction of Toca-1 with giant unilamellar vesicles. FBP17, a homologue of …
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Die Regulation aktivierender und hemmender Signalwege in humanen neutrophilen Granulozyten durch cAMP- und cGMP-erhöhende Vasodilatatoren
… spielen. Neutrophile Granulozyten enthalten VASP in hoher Konzentration. VASP ist ein bereits gut charakterisiertes Substrat der cAMP-PK und der cGMP-PK. Phosphorylierungsversuche mit cAMP-erhöhenden Substanzen ergaben eine rasche, reversible Phosphorylierung dieses Proteins an Ser-157 und …
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MOLECULAR MECHANISMS OF THE FORMATION OF ENDOCYTIC PATCHES AND THE INITIATION OF ACTIN POLYMERIZATION IN FAST ENDOPHILIN MEDIATED ENDOCYTOSIS
… local polymerization of actin. Here, we identify VASP, a leading edge specific actin polymerase, as a FEME protein and establish its interactions with the endocytic priming complex. We identify a novel non-canonical interaction between the SH3 domain of EDP and the EVH1 and EVH2 domains of VASP. …
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Elaboration of the Homer1 Recognition Landscape Reveals Incomplete Divergence of Paralogous EVH1 Domains
… the motif of a related EVH1 domain from the Ena/VASP family of actin regulatory proteins. An analysis of EVH1 binding preferences in distantly related orthologs suggests that this overlapping preference originated in the ancestral domain that gave rise to distinct Ena/VASP and Homer protein …
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Selectivity in subunit composition of EnaNASP tetramers
… The mammalian EnaNASP family members, Mena, VASP, and EVL have many overlapping activities. However, the three family members are not equivalent and each possess a number of features that are absent in the others. Unique modes of regulation and paralog-specific interacting partners point to …
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NMR AS A TOOL TO QUANTITATIVELY CHARACTERIZE MOLECULAR MECHANISMS GOVERNING ACTIN DYNAMICS AND RICE LATERAL ROOT DEVELOPMENT
… for protein-protein interaction between the Ena/VASP Homology 1 (EVH1) domain of the actin polymerize regulator Vasodilator-Stimulated phosphoprotein (VASP) and the cytoskeleton protein Zyxin was determined. This interaction is important for cellular shape, cell motility and stress fiber repair. …
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The 5' inositol phosphatase SHIP2 regulates EGF-elicited protrusion in MTLn3 cells
… a distant organ. Mena, a member of the Ena/VASP family of proteins, is upregulated in invasive populations of breast cancer cells. The Ena/VASP (enabled/vasodilator-stimulated phosphoprotein) family of proteins regulate both the geometry and dynamics of actin filament networks. Mena …
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Self-assembly of filopodia-like structures on supported lipid bilayers
… components such as Diaphanous-related formin and VASP localize to the membrane base of the structures, and bundling protein fascin to the shaft. Actin subunits assemble at the tip and translocate into the shaft. FLS assembly requires negativelycharged lipid membranes, with specific requirements …
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