Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"V(D)J recombination"”.
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STUDIES OF B CELL DEVELOPMENT AND V(D)J RECOMBINATION
… and secreted antibodies begins with the recombination of the joining (JH), diversity (DH) and variable (VH) genes in the immunoglobulin heavy chain locus. The ability to produce antibodies is restricted to the B cell lineage and is tightly regulated, starting with the temporal separation …
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The Role of Epigenetics in Regulating V(D)J Recombination and Allelic Exclusion
… gene segments. Through a process known as V(D)J recombination, genomic rearrangements occur to generate a unique antigen receptor proteins. During each stage of lymphocyte development, antigen receptor loci are epigenetically regulated. The epigenetic regulation promotes and inhibits V(D)J …
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Inferring mechanisms of V(D)J recombination using statistical inference on high-throughput immune repertoire data
… receptors through a random process called V(D)J recombination. Receptor diversity arises during recombination from the combinatorial assembly of V(D)J genes and the junctional deletion and insertion of nucleotides. While molecular experiments have established our understanding of V(D)J …
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Transcription, Editing, and Switching of Antibody Genes
… J gene segments through a process known as V(D)J recombination. Because of its stochastic nature, V(D)J recombination is expected to create self-reactive specificities. In targeted animals carrying autoantibodies, these specificities were shown to be silenced by deletion, anergy, and receptor …
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Generation and analysis of transgenetic mice expressing catalytically inactive recombination activating gene-1
The proteins encoded by the recombination activating genes (RAG1 and RAG2) are lymphoid cell-specific endonucleases that initiate V(D)J recombination; the mechanism by which the exons that encode the variable portion of both B and T cell receptors are assembled somatically from several variable …
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Regulation of the Alpha and Delta T Cell Receptor Repertoires
… Specificity is imparted by the process of V(D)J recombination. During V(D)J recombination, gene segments spread across genomic space are assembled to generate unique antigen receptors by a cut-and-paste reaction in somatic cells. The mechanisms that regulate V(D)J recombination remain unclear.In …
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Characterization of Loop Extrusion and Regulatory Elements in IgH Locus Structure and Function
V(D)J recombination is a highly ordered and stepwise process that enables antigen receptors to detect a wide variety of antigens. The Igh locus undergoes large-scale locus contraction prior rearranging in pro B cells. A series of nested chromatin loops anchored by a series of distal VH enhancers, …
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Roles of CTCF and YY1 in T Cell Receptor Gene Rearrangement And T Cell Development
… and immunoglobulins (Ig) is generated by V(D)J recombination of antigen receptor (AgR) loci. The Tcra-Tcrd locus is of particular interest because it displays a nested organization of Tcrd and Tcra gene segments and V(D)J recombination follows an intricate developmental program to assemble both …
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Spatial organisation of the immunoglobulin heavy chain locus and inter-chromosomal gene networks driving B cell development
… that undergo developmentally regulated V(D)J recombination to produce the variable region of the antibody. Gene expression depends on spatial organisation of chromatin. To ensure that all V genes have a chance to recombine, they are brought into physical proximity to the D-J region by locus …
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The Role of 53BP1 in DNA Double-Strand Break Repair
… the immune cell diversification reactions V(D)J recombination and Class Switch Recombination (CSR) require the formation of DSB intermediates, a process that is tightly controlled and strictly limited to developing B and T cells. CSR in B cells diversifies antibodies by joining DSBs between …
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ATM Deficiency -- A Multifaceted Defect in Lymphocyte Development
… be assembled through a process known as V(D)J recombination, a process that necessarily involves the generation and repair of DNA double-strand breaks made by the Rag endonuclease. Double strand breaks incurred during G1 of the cell cycle activate ATM, a PI3-kinase-like kinase that, in response …
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Vβ Recombination Signal Sequences Mediate Monoallelic And Monogenic Tcrβ Gene Assembly: Implications For The Tcrβ Repertoire And Allelic Exclusion In Health And Disease
… and assembled by RAG-endonuclease mediated V(D)J recombination to form a functional gene. TCRβ, IgH, and Igκ allelic exclusion is achieved by: assembly of an in-frame gene on one allele, transient inhibition of V(D)J recombination signaled by RAG DNA double stranded breaks (DSBs), and the …
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ANALYSIS OF B LYMPHOCYTES IN MOUSE MODEL LIGASE IV WITH HYPOMORPHIC MUTATION IN VDJ RECOMBINATION ASSOCIATED WITH GROWTH DEFECT
… are radiation-sensitive and defective in V(D)J-recombination, showing that NHEJ also functions to rejoin DSBs introduced during lymphocyte development. We have generated a knock-in mouse model with a homozygous Lig4 arginine to histidine (R278H) mutation that corresponds to the mutation …
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Investigation of the three dimensional organisation of the genome in B cell development
… is primarily generated by a form of somatic recombination known as V(D)J recombination, where different variable (V), diversity (D) and joining (J) gene segments are cut-and-pasted together. V(D)J recombination of the immunoglobulin (Ig) loci, critical to the generation of diverse antibody …
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Analysis of DIE5 and LIA5 reveals the importance of DNA repair in programmed DNA rearrangement of Tetrahymena thermophila
… proven to be an active process. Similar to V(D)J recombination during mammalian B and T cell maturation, programmed DNA rearrangement in Tetrahymena induces global DNA damage that requires proper response and repair. Through the study of LIA5 and DIE5, we show that nuclear reorganization during …
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Artemis Interacts With The Cul4A Ubiquitin E3 Ligase Complex and Regulates The Cell Cycle Progression
… stresses. Artemis has important roles in V(D)J recombination, DNA double strand breaks repair and damage-induced cell-cycle checkpoint regulation. The detailed mechanism by which Artemis mediates its functions in these cellular pathways needs to be further elucidated. My work presented here …
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Role of AID and microRNA-155 in c-myc-IgH Translocations
… The breakpoints are frequently adjacent to the recombination signal sequences (RSSs) targeted by recombinase activating genes 1 and 2 (RAG1/2) during antigen receptor assembly in pre- B cells, suggesting that these translocations might be the result of aberrant V(D)J recombination. However, in …
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DNA-PKcs/ Artemis Complex in DNA Double-Strand-Break Repair: Cryo-EM and Biochemical Studies
… it, there are two main mechanisms-- homologous recombination (HR) and non-homologous end joining (NHEJ). My PhD project focuses on NHEJ. Unlike HR, NHEJ is not limited by the cell cycle as it does not require a template for recombination. Also, NHEJ has been shown to be the preferred DSB repair …
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Study of the intracellular trafficking of the pre-T-cell receptor and its role in T-cell lineage commitment
… the two TCR variants (αβ-TCR or γδ-TCR) by V(D)J recombination. Importantly, the TCRα chain is rearranged only after correct TCRβ expression, which requires the latter chain to pair with a surrogate invariant pre-Tα chain to form the pre-TCR complex. While both TCR variants activate the same …
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Heterogeneity and Impaired IGF1R Signalling in Aged B cell Development
… B cell progenitors undergo tightly-regulated recombination of different VH, DH, and JH gene segments of the immunoglobulin heavy chain (IgH) gene loci, followed by recombination of the Ig light chain loci, to produce the heavy (μ/Mu) and light (κ/λ) chain proteins that make up an …