Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"UvrD"”.
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Single-molecule studies of mechanical and helicase-catalyzed disruption of nucleic acid duplexes
… nucleic acids and the activity of E. coli UvrD helicase. First, we use a hybrid instrument combining high-resolution optical tweezers with single-fluorophore sensitivity to observe the hybridization of ultrashort (<15 nt) DNA and RNA oligonucleotides under tension, one molecule at a time. …
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Fluorescence imaging of single molecule dynamics on long single stranded DNA
… ssDNA, (2) the unidirectional motion of E. coli UvrD helicase and (3) the diffusion of E. coli single-stranded DNA binding protein (SSB). We observed that the homology search of dsDNA on RecA filament is comprised of pauses at certain sites followed by fast transitions between the sticky sites …
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Theoretical and computational investigations into structure-function relationships of biomolecular machines at atomic resolution
… the study on Rho, we investigate a DNA helicase UvrD, which plays key roles in DNA replication and repair, by unwinding nucleic acid strands. Combining bioinformatics approaches and free energy calculations, we characterize how the UvrD helicase changes its conformation at the fork junction to …
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The role of recombinational repair in the mechanism of killing during thymine starvation in Escherichia coli
… including RecA, RecBCD, RecFOR, RecG, RuvABC and UvrD in thymineless death. I showed that stalling replication forks during thymine starvation leads to chromosomal fragmentation, partly due to replication fork disintegration. I then proposed how parallel futile repair of these chromosomal breaks …
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Die Rolle von Mutation und Rekombination in der Mikroevolution von Helicobacter pylori
… Die Ergebnisse der NER--Mutanten (uvrA-, uvrD-) zeigten eine starke Verminderung der Transformierbarkeit. Diese Verminderung hatte jedoch keinen Einfluss auf die Länge der rekombinierten DNA-Importe. Das Ausschalten des uvrA-Gens führte zudem zu einer erhöhten Sensibilität gegenüber …
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Single-molecule study of the DNA helicase regulating genomic stability
A DNA helicase, PcrA is an essential protein in gram-positive bacteria. PcrA utilizes its helicase activity and translocation activity to regulate various cellular functions including plasmid replication and counteracting deleterious recombination. Using single molecule FRET technique and …