Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 16 of 16 for “"Ussing chamber"”.
-
Development of a novel in vitro system for nasal drug delivery development
… in vitro. The existing techniques such as the Ussing chamber and cell culture all have major disadvantages. Most importantly, none of the existing techniques accurately represent the interior of the nasal cavity, with its airflow and humidity; neither do they allow the investigation of solid …
-
The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation by HSP-90
… the effect of Hsp90 on CFTR function via Ussing chamber analysis, two-electrode voltage clamp analysis, preliminary <em>in vivo</em>animal studies, and protein phosphorylation. The HEK-293 Flag-CFTR cells used in this study allows for a high degree of protein purification in quantities …
-
Sequence-Specific Gene Correction of Cystic Fibrosis Airway Basal Cells
… channel activity in the gene-corrected cells by Ussing chamber electrophysiology.</p>
-
The impact of host-microbe interactions on murine colonic secretomotor function
… colonic ion transport in the mouse, using the Ussing Chamber. I found that both probiotics appear to have converging effects on ion transport at a functional level. However, L. salivarius UCC118 may preferentially inhibit neurally-evoked ion transport. Next I examined the impact of the host …
-
TRPV4 in the Choroid Plexus Epithelium: Pathway Analysis and Implications for Cerebrospinal Fluid Production
… the mechanisms by which CSF is produced. Using Ussing chamber electrophysiology, we are able to study the role of specific channels, transporters and modulators in driving epithelial ion flux across the CP. This research aims to establish a role for TRPV4 in production and regulation of CSF, and …
-
Differentiation of Normal and Cystic Fibrosis Human Lung Epithelial Cells in a Decellularized and Reconstituted Mouse Lung
… electrical resistance measurement, histology, Ussing chamber analysis, and immunofluorescence staining of differentiation factors. I also found that this conditional reprogramming method permits cloning of human bronchial epithelial cells, and that these cells can support genetic modification …
-
SODIUM/IODIDE SYMPORTER-NIS Abundant and Important in Gastric Mucosa
… and in vitro in rat using 125I as marker. In the Ussing-chamber in vitro system both the competitive NIS-inhibitor sodium perchlorate (NaClO4) and the Na+/K+-ATPase-inhibitor ouabain attenuated gastric iodide transport from serosal to mucosal side, proving that gastric iodide se-cretion is, to a …
-
Effects of fructan source and degree of polymerization on intestinal barrier function and histomorphology characteristics in obese C57BL/6 mice
… cecum, and colon samples were collected for Ussing chamber, histomorphology, and qRT-PCR analyses. The effects of treatment were tested using the Mixed Models procedure of SAS. Among treatments, mice fed scFOS and inulin had greater (p<0.05) intestinal transmural resistance compared to mice …
-
FUNCTIONAL ADAPTATION OF THE RUMINAL EPITHELIUM
… (1000 h). Ruminal tissue was collected for Ussing chamber, barrier function, surface area measurements, and gene expression. Net 22Na+ flux (JNET-Na; 80 kBq/15 mL), the rate and pathway of mucosal to serosal 3H-acetate (JMS-acetate; 37 kBq/15 mL) and 14C-butyrate (JMS-butyrate; 74 kBq/15 mL) …
-
Effects of Chronic High Sucrose With Dietary or Drinking Inclusion on the Electrogenic Glucose Absorption in the Intestinal Tract of Mice (Mus musculus)
… were found in normal mice. Ex vivo intestinal Ussing chamber studies in normal mice characterizing the electrogenic sodium dependent glucose transport followed Hill Equation sigmoidal kinetics demonstrating low affinity, high capacity transport (Vmax of 100.8 ± 24.2 uA/cm2, K0.5 17.6 ± 0.9 mM) …
-
EFFECT OF LIPID SUPPLEMENTATION ON RUMINAL EPITHELIAL MEMBRANE FATTY ACID COMPOSITION AND SHORT-CHAIN FATTY ACID ABSORPTION
Inclusion of lipid into diets increases the energy density and, depending on the type of lipid, may alter the fatty acid (FA) composition of tissues. Effects of dietary lipid on the digestive and immune function gastrointestinal tract have been evaluated, but effects on how dietary FA affect …
-
IMMUNOMODULATION AND INTESTINAL BARRIER PROTECTION ACTIVITIES OF A NOVEL SYNTHETIC GLUCOSE ANALOGUE IN INFLAMMATORY ANIMAL MODEL OF COLITIS AND ASTHMA.
… intestinal permeability recovery observed with Ussing Chamber analysis is mediated by TJ protein protection. Results suggest that the BLF-501-mediated action involves stabilization of epithelial junction complex also in vivo. Moreover, we observed that activation of pro-inflammatory cytokines, …
-
Regulation of Intestinal Development and Function in Early-Life Pigs
… integrity. These results were supported by Ussing Chamber assays of fresh jejunal tissue. Reduced transepithelial resistance (jejunum P <0.001) combined with enhanced glucose (jejunum P <0.001) and glutamine (jejunum P <0.001) active transport at birth and postweaning described increased …
-
Ionentransportstörungen im Rektumepithel von Mukoviszidosepatienten - funktionelle Charakterisierung und Bedeutung für die Diagnostik
Mit Hilfe der Ussing-Kammer wurden Rektumbiopsien von Gesunden und Mukoviszidosepatienten elektrophysiologisch untersucht. Dabei konnten in Biopsien von Mukoviszidosepatienten charakteristische Störungen des Ionentransportes insbesondere ein Fehlen der cAMP-abhängigen Chloridsekretion nachgewiesen …
-
Osmoregulatory role of the intestine in fish physiology. Evolutionary, environmental, and toxicant implications
The earliest fishes arose circa 500 million years ago (mya) during the Ordovician and persevered throughout several mass extinction events. Unknown fishes' lines became extinct in life history (e.g., conodonts, ostracoderms, acanthodians, placoderms, etc.). It is so that extant lineages may be …
-
Early nutrition affects intestinal development and immune response in the neonatal piglet
… Intestinal permeability was measured using the Ussing chamber. Route of delivery affect some of these parameters. CD piglets had greater glucose and glutamine transport at d14 compared to VD piglets. VD piglets also had increased jejunal basal Isc compared to CD piglets at all time points. In …