Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"Ussing"”.
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Untersuchungen zur Interaktion von Quercetin-Glukosiden mit dem intestinalen Glukose-Carrier (SGLT1)
… der Ratte untersucht, zum anderen mit Hilfe der Ussing-Kammer überprüft, ob der SGLT1 am Transport von Isoquercitrin und Rutin in die Dünndarmmukosazellen beteiligt ist. Es konnte gezeigt werden, dass beide Quercetin-Glukoside die mukosale MDG-Aufnahme über den Glukose- Transporter signifikant …
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Mechanisms of Prostaglandin-Stimulated Recovery of Mucosal Barrier Function in the Ischemia-Injured Porcine Intestine: Role of Intestinal Ion Transport
… porcine ileal mucosa was mounted in Ussing chambers. Short circuit current (Isc) and transepithelial resistance (TER) were measured in response to PGE2 and pharmacologic inhibitors of epithelial Cl- channels. Overall, results from these studies demonstrate that ClC-2-mediated …
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Ionentransportstörungen im Rektumepithel von Mukoviszidosepatienten - funktionelle Charakterisierung und Bedeutung für die Diagnostik
Mit Hilfe der Ussing-Kammer wurden Rektumbiopsien von Gesunden und Mukoviszidosepatienten elektrophysiologisch untersucht. Dabei konnten in Biopsien von Mukoviszidosepatienten charakteristische Störungen des Ionentransportes insbesondere ein Fehlen der cAMP-abhängigen Chloridsekretion nachgewiesen …
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Isolierung gastrointestinal wirksamer Inhaltsstoffe aus Casimiroa tetrameria MILLSP., einer yukatekischen Arzneipflanze der Maya (Mexiko)
… Untersuchungen dieser Art wurde das Modellsystem USSING-Kammer verwendet. Am distalen Colon des Kaninchens zeigte der Rohextrakt serosal eine dosisabhängige antisekretorische Wirkung bezüglich der PGE2-induzierten Cl--Sekretion. Durch eine Fraktionierung konnte nachgewiesen werden, daß die …
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Quantitative Bestimmung der 9-minus-Splice-Varante in CFTR-mRNA aus Rektumschleimhautbiopsie und deren Abhängigkeit von der Länge des Poly-T-Traktes in Intron 8 des CFTR-Gens
… Befunden aus Untersuchungen in der Ussing-Kammer statt. Hier zeigte sich eine krankheitsmodulierende, bzw. auslösende Bedeutung des Poly-T-Polymorphismus.
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Development of a novel in vitro system for nasal drug delivery development
… in vitro. The existing techniques such as the Ussing chamber and cell culture all have major disadvantages. Most importantly, none of the existing techniques accurately represent the interior of the nasal cavity, with its airflow and humidity; neither do they allow the investigation of solid …
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The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation by HSP-90
… the effect of Hsp90 on CFTR function via Ussing chamber analysis, two-electrode voltage clamp analysis, preliminary <em>in vivo</em>animal studies, and protein phosphorylation. The HEK-293 Flag-CFTR cells used in this study allows for a high degree of protein purification in quantities …
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CFTR klórgöng í ristilþekju hænsnfugla (Gallus gallus)
… í burtu. Þekjunni var þvínæst komið fyrir í Ussing baði sem var svo fyllt með Krebs fosfat búffer. Efnunum glúkósa, NPPB (klórgangahindi) og forskolin (CFTR örvari) bætt út í baðið í mismunandi röð til að kanna virkni klórganganna. Niðurstöður: Glúkósi hafði engin marktæk áhrif á jónaflutning …
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Sequence-Specific Gene Correction of Cystic Fibrosis Airway Basal Cells
… channel activity in the gene-corrected cells by Ussing chamber electrophysiology.</p>
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The impact of host-microbe interactions on murine colonic secretomotor function
… colonic ion transport in the mouse, using the Ussing Chamber. I found that both probiotics appear to have converging effects on ion transport at a functional level. However, L. salivarius UCC118 may preferentially inhibit neurally-evoked ion transport. Next I examined the impact of the host …
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TRPV4 in the Choroid Plexus Epithelium: Pathway Analysis and Implications for Cerebrospinal Fluid Production
… the mechanisms by which CSF is produced. Using Ussing chamber electrophysiology, we are able to study the role of specific channels, transporters and modulators in driving epithelial ion flux across the CP. This research aims to establish a role for TRPV4 in production and regulation of CSF, and …
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Nerve growth factor regulates Na+ transport in human airway epithelial cells
… several imaging techniques. Cells were placed in Ussing chambers to obtain transepithelial voltage (Vt, -7.1 +/- 3.4 mV), short-circuit current ( Isc, 5.9 +/- 1.0 muA), and transepithelial resistance (Rt, 750 Ohm x cm2), and to measure responses to ion transport inhibitors. Apical and basolateral …
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Differentiation of Normal and Cystic Fibrosis Human Lung Epithelial Cells in a Decellularized and Reconstituted Mouse Lung
… electrical resistance measurement, histology, Ussing chamber analysis, and immunofluorescence staining of differentiation factors. I also found that this conditional reprogramming method permits cloning of human bronchial epithelial cells, and that these cells can support genetic modification …
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Nutritional strategies to control mycotoxin damages in swine
… threat particularly in swine husbandry. The Ussing chambers technique was applied in the present study that for the first time to investigate in vitro the permeability of OTA and FB1 through rat intestinal mucosa. Results showed that OTA and FB1 were not absorbed from rat small intestine …
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Characterization of the airway epithelial bioelectric mechanisms associated with the effects of epithelium-derived relaxing factor in guinea pig-isolated trachea
… in guinea-pig tracheal segments mounted in Ussing chambers. The data from these studies indicate that apical Na + channels and the basolateral Na+-K+-2Cl - cotransporter are intimately involved in the epithelial bioelectric response to elevated mucosal tonicity, and thus, may play an …
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SODIUM/IODIDE SYMPORTER-NIS Abundant and Important in Gastric Mucosa
… and in vitro in rat using 125I as marker. In the Ussing-chamber in vitro system both the competitive NIS-inhibitor sodium perchlorate (NaClO4) and the Na+/K+-ATPase-inhibitor ouabain attenuated gastric iodide transport from serosal to mucosal side, proving that gastric iodide se-cretion is, to a …
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Pharmacological characterisation of selective Y4 and dual Y2/Y4 receptor agonists: novel targets for putative anti-obesity therapies
… bioassays, where preparations were mounted in Ussing chambers and changes in short-circuit current (Isc) recorded. In addition, receptor specificity of the most promising agonists was dissected using Y1 and Y2 antagonists. Furthermore, an assessment of Y4 receptor desensitisation was made by …
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Effects of agro-industrial by-products on health and growth performance of livestock: Studies on white grape marc extract in broilers and three different fibrous by-products in weaned piglets
… nor in breast muscle antioxidant capacity. Ussing chamber analysis revealed reduced jejunal tissue conductance after glucose addition in the medium and high GME groups, suggesting subtle effects on epithelial transport. Overall, GME exerted no adverse effects at any tested inclusion level. …
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Protektiver Effekt von 6-Shogaol, Ellagsäure und Myrrhe auf die intestinale epitheliale Barriere
… Widerstands (TER) und der Fluxmessung in der Ussing-Kammer bestimmt. Dazu werden die HT-29/B6- und Caco-2-Monolayer mit den Pflanzenkomponenten (6-Shogaol, Ellagsäure, Myrrhe), dem proinflammatorischen Zytokin TNF-α oder der Kombination von beiden Subsztanzen für 24 oder 48 h behandelt. …
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Effects of fructan source and degree of polymerization on intestinal barrier function and histomorphology characteristics in obese C57BL/6 mice
… cecum, and colon samples were collected for Ussing chamber, histomorphology, and qRT-PCR analyses. The effects of treatment were tested using the Mixed Models procedure of SAS. Among treatments, mice fed scFOS and inulin had greater (p<0.05) intestinal transmural resistance compared to mice …
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