Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Upstream open reading frames"”.
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The Regulation of NAP4 in Saccharomyces cerevisiae
… (1) transcriptional regulation via two short upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of …
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Development of a ribosome profiling protocol to study translation in the yeast Kluyveromyces marxianus
… of mRNA translation including recoding (e.g. +1 frameshifting), ribosome stalling and differential gene expression between multiple conditions. In addition, as RPFs can be mapped back to the genome, novel translated ORFs may be discovered including novel protein coding genes and regulatory …
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A study of mammalian microRNA-mediated repression of gene expression by ribosome profiling
… addition, we observed widespread translation of upstream open reading frames initiated from canonical and non-canonical start codons. These upstream open reading frames are also translationally repressed by miR-155.
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An inductive logic programming approach to learning which uORFs regulate gene expression.
Some upstream open reading frames (uORFs) regulate gene expression (i.e. they are functional) and can play key roles in keeping organisms healthy. However, how uORFs are involved in gene regulation is not het fully understood. In order to get a complete view of how uORFs are involved in gene …
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The effect of alternative splicing on key regulators of the integrated stress response
… with different 5'-leaders encoding inhibitory upstream open reading frames that are critical for translational control of CHOP during stress. This study suggests that alternative splicing can play an integral role in the implementation and regulation of key factors in the Integrated stress …
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Understanding regulation of mRNA by RNA binding proteins
… foot printing show that actively translated upstream open reading frames (uORFs) are enriched in transcription factor mRNAs and predict mRNA repression by NMD, while poorly translated mRNAs escape repression.
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Mitochondrial parts, pathways, and pathogenesis
… measurements to demonstrate a widespread role of upstream open reading frames (uORFs) in blunting translation of mitochondrial and other cellular proteins. Next we used the mitochondrial protein inventory to identify genes underlying inherited diseases of mitochondrial dysfunction. In …
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Characterization of KPAP, a Novel Pokeweed Antiviral Protein Isoform
… SRL. My results show the presence of predicted upstream open reading frames (uORFs) in the long leader intron, perhaps belonging to retrotransposons. The enzymatic activity recorded from KPAP in an in vitro translation assay resembles the activity of PAP-I. Moreover, the exclusive expression of …
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Post-Transcriptional Regulation of Mammalian Gene Expression In Non-Coding Region of Target Rna
… protein expression. We found that two small upstream open-reading frames (uORFs) in the 5’UTR of TUSC2 could markedly inhibit the translational initiation of TUSC2 protein by interfering with the “scanning” of the ribosome initiation complexes. Site-specific stem-loop array reverse …
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The mechanisms regulating the transcription factor ATF5 and its function in the integrated stress response
… stress. ATF5 translational control involves two upstream open reading frames (uORFs) located in the 5′-leader of the ATF5 mRNA, a feature shared with ATF4. Mutational analyses of the 5′-leader of ATF5 mRNA fused to a luciferase reporter suggests that the 5′-proximal uORF1 is positive-acting, …
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Comparative analysis of African and Asian/American Zika virus strains at the transcriptional and translational level
… profile of each virus. Two previously overlooked upstream open reading frames (uORFs) in the 5′ untranslated region (UTR) of the American ZIKV genome were identified, initiating at non-canonical CUG and UUG codons; and a single uORF in the 5′UTR of the African ZIKV, initiating at a CUG start …