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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"Unnatural Amino Acids"”.
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Using Unnatural Amino Acids and Nonnative Cofactors for Metalloprotein Design and Engineering
Different positions for anchoring the salen cofactor to the Mb scaffold were observed to affect both the rate and selectivity of the artificial enzyme in a modular and accumulative fashion. Molecular dynamic simulations indicate that these changes may be due to the steric effects of the linker arms …
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Metalloprotein engineering with unnatural amino acids: application in functional heme-copper oxidase and azurin
… provides us the ability to mutate certain amino acid in metalloproteins to all other natural amino acids through site-directed mutagenesis. However, site-directed mutagenesis is restricted to 20 natural amino acids, only half of which could coordinate to metal. Incorporation of unnatural …
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Towards The Development Of Intrinsically Fluorescent Unnatural Amino Acids For In Vivo Incorporation Into Proteins
Acridonylalanine (Acd) is a fluorescent unnatural amino acid that can site specifically label a protein of interest during protein translation. Acd’s incorporation relies on an evolved tRNA synthetase that specifically charges Acd to its cognate tRNA. As the protein mRNA sequence is being …
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Functionalizing Branched Peptides with Unnatural Amino Acids Toward Targeting HIV-1 RRE RNA and Microbials
… several branched peptide libraries containing unnatural amino acids to target the high-affinity binding site of RRE RNA (RRE IIB), with the idea that branching in peptides can provide multivalent contacts with folded RNA structures and boost binding affinity and selectivity for the target. …
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Thermodynamic and Spectroscopic Investigations of Novel Antimicrobial Peptides containing Unnatural Amino Acids with Model Membrane Systems
… of antimicrobial peptides that incorporate unnatural amino acids to impart specific physicochemical properties onto these peptides. Circular Dichroism (CD), isothermal titration calorimetry (ITC) and calcein leakage assays were conducted and it was determined that there are two distinctly …
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Designing Novel Blue Copper and Purple CuA Centers in Azurin With Natural and Unnatural Amino Acids
Finally, loop-directed mutagenesis was used to incorporate a 2Fe-2S cluster binding loop from human ferrochelatase into azurin. Preliminary iron binding studies indicate the assembly of a 4Fe-4S or a 2Fe-2S iron sulfur cluster.
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Amphiphilic Cell-Penetrating Peptides Containing Natural and Unnatural Amino Acids as Drug Delivery Tools and Antimicrobial Agents
<p>Cell-penetrating peptides containing arginine as positively charged residues and tryptophan or diphenylalanine as hydrophobic residues were synthesized. The synthesis was accomplished through the Fmoc solid-phase peptide synthesis in the presence of HBTU and DIPEA. The side-chain protected …
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Investigation of the mechanism of radical propagation in E. coli ribonucleotide reductase by site-specific incorporation of unnatural amino acids
… expressed protein ligation and suppressor tRNA/aminoacyl-tRNA synthetase (RS) methodologies to site-specifically insert unnatural tyrosine analogues into 12 and a2, at residues believed to be involved. On the basis of results with the radical traps 3,4-dihydroxyphenylalanine (DOPA) and …
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The Ribosome Discriminates the Structure of the Amino Acid at its Peptidyl-Transferase Center
… original Adaptor Hypothesis view both the amino acid and the tRNA body as passive participants during aminoacyl-tRNA (aa-tRNA) selection by the ribosome. Recent experimental evidence investigating tRNA mutants that miscode due to mutations that lie outside the anticodon as well as data from …
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Adiciones 1,4-conjugadas a deshidroalaninas quirales para la síntesis de aminoácidos no naturales
… different serine-derived bicyclic chiral dehydroamino acids, developed in the research group in which this work has been carried out (QuiBi at University of La Rioja). α,β-Dehydroamino acids are a type of electrophiles widely employed in 1,4-conjugate additions with nucleophiles of different …
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Methodologies For Attaching Polypyridyl Ligands Into Amino Acids And Synthesis And Biological Evaluation Of Novel Light Activated Peptidomimetic Cysteine Protease Inhibitors Caged By Ruii(bpy)2
… for attaching polypyridyl ligands into unnatural amino acids. The first chapter describes the different possibilities for attaching metal ligands to peptides and their applications as potential imaging and therapeutic agents covered so far in the literature. It is followed in the second …
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Expanding the genetic code of a mouse
… has used the site-specific insertion of unnatural amino acids into proteins(Greiss and Chin, 2011). An aminoacyl-tRNA synthetase and a tRNA are used to specifically insert the unnatural amino acid during mRNA translation, in response to an amber stop codon (UAG) placed at a user-defined …
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Experimental and Computational Advancements in Peptidomimetic Ligand Discovery
… libraries, including the incorporation of unnatural amino acids or the modification of library structure through macrocyclization. This thesis will focus on the development of experimental and computational tools to analyze affinity selection datasets more efficiently and thoroughly. We …
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Translational Incorporation of Unsaturated Amino Acids
… synthesised using twenty standard proteinogenic amino acids. The unique linear sequences of these monomeric building blocks dictate the structure and functionality of the translated protein. Misincorporation of unnatural amino acids into this biosynthetic process is rare due to the high fidelity …
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Translational Incorporation of Unsaturated Amino Acids
… synthesised using twenty standard proteinogenic amino acids. The unique linear sequences of these monomeric building blocks dictate the structure and functionality of the translated protein. Misincorporation of unnatural amino acids into this biosynthetic process is rare due to the high fidelity …
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Chemical Tools for Exploring IFITM3 S-Palmitoylation and Mechanism
… for site-specific IFITM3 labeling with unnatural amino acids for bioorthogonal imaging and covalent protein crosslinking in mammalian cells in Chapter 3. Using the pyrrolysyl-tRNA synthetase (PylRS)/Pyl tRNACUA system, I showed that unnatural amino acid can be site-specifically …
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Rewriting the genome of Escherichia coli
… and these may be amenable for reassignment into unnatural amino acids, in synergy with parallel efforts to engineer the protein translation machinery. Recoding genomes is subject to both biological and technical challenges. First, synonymous codon choice genome-wide is not trivial, and …
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Application of Pd Catalyzed Alkylation: Synthesis of Bicyclic Furans, Isoxazolines and New Cyclopentane Amino acid Analogs
… furans, isoxazolines and new cyclopentane amino-acid analogs in an enantioselective manner. The stereochemical outcome of these reactions is influenced by desymmetrization catalyzed by hydrolytic enzymes namely lipases. Chapter 1 reviews the recent advances in the field of palladium …
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From Probes to Cell Surface Labelling: Towards the Development of New Chemical Biology Compounds and Methods
… chemistry, often by virtue of small molecules or unnatural amino acids. Much insight has been gained into the mechanisms of biological processes with regards to protein structure and function, metabolic processes and changes between healthy and diseased states. As an ever expanding field, …
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Investigating and expanding the functionality of RNA catalysts: Studies of the hepatitis delta virus, the hammerhead, and the aminoacyl-tRNA synthetase-like ribozymes
… ribozyme that mimics the function of the natural aminoacyl-tRNA synthetase. The design was based on the sequence of a naturally occurring riboswitch and an in vitro selected ribozyme that could charge tRNA with chemically synthesized unnatural amino acid substrates. The engineered ribozyme …
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