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Showing 1 to 16 of 16 for “"Uniporter"”.
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Genetic characterisation of the Drosophila Mitochondrial Calcium Uniporter in physiological and neurodegenerative contexts
… process has only recently been described. The uniporter is comprised of MCU, the pore-forming component; EMRE, which coordinates the complex architecture and is required for in vivo calcium uptake; and MICU1-3, which regulate the flow of ions. Animal models have emerged for some uniporter …
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The Effects of S-glutathionylation on the Structure and Function of the Mitochondrial Calcium Uniporter (MCU)
… matrix is regulated by the mitochondrial calcium uniporter (MCU). The amino (N)-terminal domain (NTD) of MCU is a regulatory component of the channel. S-Glutathionylation of Cys97 on the MCU-NTD leads to robust MCU activation and increased matrix Ca2+. Here, I characterized the biophysical and …
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Mitochondrial Calcium Uptake: LETM1 and MICU1 Are Mitochondrial Proteins That Regulate Mitochondrial Calcium Homeostasis and Cellular Bioenergetics
… Ca2+ uptake distinct from the mitochondrial Ca2+ uniporter (MCU) pathway (Jiang et. al., 2009). However, a controversy exists regarding the function of LETM1 (Nowikovsky et. al., 2004). Therefore, I asked if LETM1 played a role in mitochondrial Ca2+ uptake and if LETM1 regulated cellular …
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Spatio-Temporal Calcium Dynamics of the Interstitial Cells of Cajal
… (IP3R) release, and mitochondria, through the uniporter. In the intestinal regions, a non-specific cation channel (NSCC) is associated with the membrane depolarizations, and is inhibited by intracellular calcium. One theory proposes that the integrated calcium transport occurs within specific …
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Celastrol induces paraptosis-like cell death via mitochondrial Ca2+ overload in breast cancer cells
… cell death. Inhibition of mitochondrial Ca2+ uniporter employing ruthenium red and IP₃ receptor employing 2-APB very effectively blocked celastrol-induced paraptosis-like cell death. Taken together, our results suggest that Ca²⁺ overload into the mitochondria via mitochondrial Ca2+ uniporter …
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Identification and functional characterisation of different inner mitochondrial membrane supercomplexes
… between CIV and the mitochondrial calcium uniporter complex (MCUC), responsible for mitochondrial calcium uptake and homeostasis. Our data, indeed, showed a specific physical interaction between this ETC enzyme and various subunits of MCUC in first and second dimension blue native PAGE, …
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Quercetin and chloroquine synergistically induces cell death in glioma cells via the increase of Ca2+ and ROS levels
… and RU-360 (an inhibitor of mitochondrial uniporter), suggesting that the release of Ca2+ from the endoplasmic reticulum and mitochondria Ca2+ influx may be critically involved in this cell death. In addition, combined treatment with quercetin and CQ increased ROS levels and their …
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Funktionelle Charakterisierung der Transportproteine für Organische Kationen rOCT1 und hOCT2 unter besonderer Berücksichtigung der cis-/trans-Asymmetrie von rOCT1
… und Hemmstoffen. Beide Transporter können als Uniporter klassifiziert werden, d.h. sie besitzen keine obligate Kopplung an ein Austausch- oder Cosubstrat wie z.B. Natrium, Protonen oder andere organische Kationen. Darüberhinaus zeigen sie das für Transportproteine typische und von Kanälen …
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Molecular Determinant of Mitochondrial Shape Change
… selective channel, the Mitochondrial Calcium Uniporter (MCU). Mitochondrial Ca2+ (mCa2+) is utilized by the matrix dehydrogenases for maintaining cellular bioenergetics. The TCA cycle-derived NADH and FADH2 are mCa2+ dependent thus, feed into the electron transport chain (ETC) to generate ATP. …
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Mitochondrial response to axonal injury
… uptake using RU360, a mitochondrial calcium uniporter inhibitor, prevented injury induced fission. This phenomenon was also observed in vivo. These studies indicate that following injury, both in vivo and in vitro, axonal mitochondria undergo increased fission, which may result in an ATP …
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Investigation into the destructive and adaptive responses of neural cells to stress
… a manner dependent on the mitochondrial calcium uniporter (MCU), as well as increase their capacity to buffer their intracellular redox balance. Finally, I have studied transcriptional programs in astrocytes triggered by neurons and neuronal activity to better understand adaptive signaling …
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Comparative analysis of the role of redox active molecules on bioenergetically active membranes
… and found that the mitochondrial calcium uniporter played an important role in vanadium induced mitochondrial swelling. We further investigated the differences in species and oxidation state on function. We tested the difference between VV and VIV on mitochondrial swelling and found that …
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The Role of Calcium Signalling in Autophagy
… knockdown of the mitochondrial Ca<sup>2+</sup> uniporter. The triggering of autophagy under these conditions was due to reduced cellular ATP levels. These data suggest that Ca<sup>2+</sup> signals arising from InsP<sub>3</sub>Rs suppress autophagy. <br></br><br></br> Additional studies used a …
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Functional characterization of the human renal organic anion transporter 3 (hOAT3) and comparison to hOAT1
… in seiner ursprünglich postulierten Funktion als Uniporter anionische Substrate entgegengesetzt, also von der Zelle ins Blut transportieren. In der vorliegenden Studie haben wir daher die Triebkräfte des hOAT3 erneut untersucht. Darüberhinaus wurde der hOAT3 funktionell mit dem hOAT1 verglichen, …
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Investigating the role of mitochondrial Ca2+ in in vivo models of neurodegenerative diseases
… of Ca2+ influx through the mitochondrial Ca2+ uniporter (MCU) complex and efflux through the Na+/Ca2+ transporter (NCLX). This balance is further influenced by Ca2+ transfer from the endoplasmic reticulum (ER) to mitochondria. Mitochondrial Ca2+ is a master regulator of essential cellular …
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Computational study of the structure and function of membrane transport proteins
… present a unified mechanism of transport for the uniporter class of transporters. A second class of transporters namely symporters that couple the electrochemical gradient of a co transported ion to perform the uphill transport of the substrate was also studied. Using the H+-coupled Xylose …