Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"Ultra Performance Liquid Chromatography"”.
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Skysčių chromatografija–tandeminė masių spektrometrija antibiotikų nustatymui maisto produktuose /
High–performance liquid chromatography (HPLC) and/or ultra-performance liquid chromatography (UPLC) techniques coupled with tandem mass spectrometry (MS/MS) were developed for the determination of common antibiotics (chloramphenicol, sulfonamides and nitroimidazoles together with their metabolites) …
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Stability of heroin metabolites and oxycodone in rat hair and liver during decomposition
… were analysed in hair samples by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Oxycodone concentrations in both hair and liver decreased as the interment period and decomposition of the carcasses progressed. 6-Monoacetylmorphine was not detected in …
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Pharmacokinetic Evaluation of a Novel Compound, Sn79, a Putative Sigma-2 Receptor Antagonist, By Intravenous and Oral Administration in Rats
… and validation of a bio-analytical method using ultra performance liquid chromatography-mass spectrophotometry to selectively separate and identify SN79 in biological matrix was a crucial part of this project. Determination of various physicochemical parameters including aqueous solubility, …
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Pharmacokinetics and pulmonary distribution of Draxxin® (tulathromycin) in healthy adult horses
… lavage (BAL) samples were determined by ultra-performance liquid chromatography tandem mass spectrometry and pharmacokinetic parameters calculated using noncompartmental analysis. Following SC and IM administration, all horses exhibited sweating, discomfort, and periods of recumbency. As …
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Molecular target identification of antimalarial drugs using proteomic and metabolomic approaches
… For this purpose an ion pair reversed phase ultra-performance liquid chromatography in tandem with mass spectrometry method was developed and validated. In addition, the apicoplast is an organelle present in the malaria parasite and other apicomplexan parasites. It was demonstrated that the …
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Spatial and temporal analysis of pharmaceutical concentrations and chitobiase activity in the Upper Tennessee River
… developed utilizing solid phase extraction and ultra-performance liquid chromatography (UPLC) interfaced with an electrospray ionization, tandem mass spectrometer (MS/MS). This method yielded reproducible quantitation at the low parts per trillion (ng/L) level for all 14 analytes (acetaminophen, …
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A Catharanthus roseus mutant with trace levels of secologanin and monoterpenoid indole alkaloids does not express BIS1/BIS2 transcription factors and fails to activate iridoid biosynthesis.
… had been screened with a simple TLC (thin layer chromatography) to identify mutants with altered MIA profiles yielded one plant with trace MIA production. This trace MIA status was confirmed using UPLC-MS (Ultra Performance Liquid Chromatography and Mass Spectrometry) and the plant had a thick, …
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Functional and ecological investigations in Cucurbita: factors mediating fruit infections and insect interactions
… greenhouse‐grown flowers of cultivars by ultra-performance liquid chromatography to quantify cucurbitacin B levels. Both flower availability and cultivar identity strongly influence beetle abundance. Although flowers provide essential feeding sites, inherent cultivar traits significantly …
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Heterologous production of recombinant peptidylglycine α-amidating monooxygenase for the production of biosimilar α-amidated peptides
… AGG chromophore synthesised was identified with ultra-performance liquid chromatography mass-spectroscopy (UPLC-MS). PAM activity was measurable with glyoxylate between 25 µM and 1600 µM with the AGG assay. Furthermore, the activity of PHM alone was measured by the inclusion of an alkaline …
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Forensic Analysis of Trace Evidence Fibers and Drugs of Abuse In Biological Matrices Using Chromatographic and Mass Spectrometric Methods
… fibers by micro-extractions of dyes followed by liquid chromatography (LC) or capillary electrophoresis (CE) with UV/visible diode array (DAD) or mass spectrometric (MS) detection is described. Separation and characterization of acid, basic and disperse dyes from nylon, acrylic and polyester …
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A More Efficient Method for Extracting and Analyzing Pesticides in Baby foods
… the extracts with standard instruments like High-performance liquid chromatography-photodiode array (HPLC-PDA), Gas chromatography-mass spectrometer (GC/MS), and Ultra-performance liquid chromatography-ultraviolet (UPLC/UV). The focus will be on improving the extraction process. Starting with …
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Measuring steroids from dried blood spots using tandem mass spectrometry to diagnose congenital adrenal hyperplasia
… and rapid assay to measure seven steroids using liquid chromatography mass spectrometry (LC-MS/MS) in DBS. An optimized DBS sample preparation method was employed without the need of derivatization. A LC-MS/MS assay was developed and optimized using reverse phase-ultra performance liquid …
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Development of ion mobility mass spectrometry deconvolution strategies for use in data-independent acquisition proteomics.
… of IM-MS deconvolution to HDMSᴱ acquisition. Ultra-performance liquid chromatography (UPLC) was used with HDMSᴱ for high-throughput bottom-up proteomics analysis of rat brain tissue. The combined use of broadband IM-MS deconvolution and commercial HDMSᴱ data processing software allowed more …
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Investigation of circulatory and tissue-specific metabolomic biomarkers in valvular heart disease using mass spectrometry
… was performed on serum and valve tissues using ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry. In-situ biomarker localisation was performed with matrix assisted laser desorption ionisation mass spectrometry imaging. Univariate and multivariate …
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Metabolomic Discrimination of Near Isogenic Low and High Phytate Soybean [GLYCINE MAX (L.) MERR.] Lines
… metabolite profiles were determined using ultra performance liquid chromatography mass spectrometry and metabolomic differences between the low and normal phytate lines were identified. The low phytate double mutant did not contain C22 glucose terminated Group A soyasaponins and almost …
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Colonic metabolism of dietary grape seed extract: Analytical method development, effect on tight-junction proteins, tissue accumulation, and pan-colonic pharmacokinetics
… tract. First, a new high-throughput Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry method was developed to efficiently analyze PCs and an extensive profile of their microbial metabolites. This method is sufficiently sensitive and effective in simultaneously …
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Improving Non-nutritive Sweetener Study Design Methodology
… NNS biomarker methodology was developed using ultra performance liquid chromatography (UPLC-MS/MS), which analyzes each sample for the presence of NNS and related metabolites. This method observed a range from very strong presence of NNS to not detectable, indicating that this biomarker could …
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Aqueous humor concentration and prostaglandin E2 suppression efficacy of topically applied ophthalmic ketorolac 0.5% and diclofenac 0.1% solutions in dogs with cataract
… and prostaglandin E2 (PGE2) concentrations via ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) and enzyme-linked immunoassay (ELISA) analysis, respectively. Results: Median aqueous humor drug concentrations were significantly higher in dogs treated with ketorolac 0.5% …
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Characterization of Microorganisms of Interest to Homeland Security and Public Health Utilizing Liquid Chromatography/Mass Spectrometry
Liquid chromatography/quadrupole time of flight mass spectrometry (LC/QTOF MS) utilizing electrospray ionization was employed to monitor protein expression in Escherichia coli and Shigella organisms. Automated charge state deconvolution, spectral subtraction and spectral mirroring were used to …
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Examining the Roles of Membrane Lipids and Secreted Bioactive Lipids on Immune System Cell Function: Studies on Mast Cells and Platelets
… dissertation focuses on developing and applying ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methods to address the challenge of quantifying mast cell-secreted bioactive lipids in complex cell cultures as well as understanding the roles that membrane phospholipids …
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