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Showing 1 to 20 of 66 for “"Ubiquitin ligases"”.
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Role of Ubiquitin and Ubiquitin Ligases in Retroviral Budding
… bind ALIX, and PPxY domains bind Nedd4-like HECT ligases, a class of ubiquitin ligase enzymes. The functional link between HECT ligases and the VPS pathway has not been defined, but multiple lines of evidence suggest that ubiquitin is generally important for late domain activity. Retroviral Gag …
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Sumo and Ubiquitin Ligases Regulate Hepatocyte Nuclear Factor - 1 Beta Transcriptional Activity
… with HNF-1β The SUMO E2 ligase, Ubc9, SUMO E3 ligases, PIAS1 and PIASgamma , and the ubiquitin E3 ligase, Arkadia, were isolated as putative interacting proteins. SUMOylation of transcription factors affects their subcellular localization, intracellular transport, stability, and transcriptional …
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Insights into BTB-Cul3 Ubiquitin Ligases from the Structures of SPOP-Substrate Complexes
<p>Cullin-Ring ubiquitin ligases (CRLs) are E3 complexes that specifically recognize substrates through substrate adaptors. In the largest CRL subfamily, Cul3 binds a BTB domain, and a protein-interaction domain such as MATH recruits substrates for ubiquitination. Here we present biochemical and …
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Ligandability of protein-protein interactions and surfaces on Cullin RING E3 ubiquitin ligases
Cullin RING E3 ubiquitin ligases (CRLs) function in the ubiquitin proteasome system by catalysing the transfer of ubiquitin from E2 conjugating enzymes to specific substrate proteins. CRLs are large dynamic multi-subunit complexes that control the fate of many proteins in cells and, therefore, …
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Functional analysis of Shigella encoded IpaH E3 ubiquitin ligases in cell-autonomous immunity
… amongst them a family of twelve bacterial E3 ubiquitin ligases (IpaH) that are structurally unrelated to eukaryotic enzymes. IpaH ligases are injected into the host cytosol via the bacterial type III secretion system (T3SS) to manipulate the host cell and counteract anti-bacterial defense …
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Mechanistic Studies of the Activation of Ubiquitin-Conjugating Enzymes by Ring-Type Ubiquitin Ligases
Ubiquitination, modification with ubiquitin, is a post-translational regulation of proteins in eukaryotes. Ubiquitin-activating enzymes (E1) activate ubiquitin and form thioester linkages with ubiquitin, which are then transferred onto ubiquitin-conjugating enzymes (E2). Ubiquitin-conjugating …
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Understanding and Engineering Chemically Activated Ubiquitin Ligases for High-throughput Detection, Quantification, and Control of Molecules in Yeast
… encoded biosensor engineered from the E3 ubiquitin ligase to detect and quantify intracellular auxin in a Saccharomyces cerevisiae model. The biosensor has been applied to begin studying auxin metabolism and biosynthesis in yeast as well as better understand the plant auxin co-receptor …
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The Expanding Diversity of Plant U-box E3 Ubiquitin Ligases in Arabidopsis: Identifying AtPUB18 and AtPUB19 Function during Abiotic Stress Responses
… As part of these diverse cellular functions, ubiquitin-mediated proteolysis has emerged to be an important process involved in how plant signalling pathways can be regulated in response to such cues. Of the three enzymes involved in linking ubiquitin to protein targets, E3 ubiquitin ligases …
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The role of Ubiquitin ligases Nedd4-1 and Nedd4-2 in the brain and their crosstalk with alpha-synuclein and other dementia-related proteins
… to target α-synuclein for breakdown through ubiquitination, both in cellular models, and in fly and rat PD models. It is therefore of interest to research the effect of deleting Nedd4 family members in dopaminergic neurons. In order to investigate the function of some Nedd4 family members …
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Mechanisms of repression of the transcription factor NRF2 by KEAP1- and B-TrCP-dependent ubiquitin ligases and how the dysregulation of NRF2 contributes to lung cancer progression
Lung cancer is the leading cause of cancer related mortality worldwide and since the discovery of the important role that NRF2 plays in regulating the expression of phase II drug metabolism genes, NRF2 has been a highly studied therapeutic target. NRF2 signalling is often elevated in lung cancer …
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Regulation of Protein Degradation In The Heart By Amp-Activated Protein Kinase
<p>The degradation of proteins by the ubiquitin proteasome system is essential for cellular homeostasis in the heart. An important regulator of metabolic homeostasis is AMP-activated protein kinase (AMPK). During nutrient deprivation, AMPK is activated and intracellular proteolysis is enhanced …
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Regulation of Protein Degradation In The Heart By Amp-Activated Protein Kinase
<p>The degradation of proteins by the ubiquitin proteasome system is essential for cellular homeostasis in the heart. An important regulator of metabolic homeostasis is AMP-activated protein kinase (AMPK). During nutrient deprivation, AMPK is activated and intracellular proteolysis is enhanced …
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Regulation of Apoptosis by XIAP Ubiquitin- Ligase Activity
… IAPs contain RING domains that function as E3 ubiquitin-ligases to regulate the abundance of IAPs themselves and their binding partners by engaging the ubiquitin system. The precise physiological function of individual mammalian IAPs and their role as E3- ubiquitin ligases in situ remain …
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Investigating new roles of the ubiquitin proteasome system in cell death and innate immune signalling
… we explored the role of some regulators of the ubiquitination and proteasome systems in cell death and innate immune signalling pathways in macrophages. Initial studies focused on a form of regulated cell death termed necroptosis, a type of cell death mediated by the receptor-interacting protein …
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Biochemical and Structural Characterization of Divergent Members of the Protein Kinase Family
… glutamylation to inactivate the SidE ubiquitin ligases. SidJ becomes activated upon translocation into the host cell and uses cellular ATP and glutamic acid to inactivate another bacterial effector family, the SidE all-in-one ubiquitin ligases. By determining cryo-EM structures of the …
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Biochemical Characterization of IpaH E3 Ubiquitin Ligase Effector Proteins and Their Host Substrates
… including Salmonella and Yersinia secreted E3 ubiquitin ligases into the host cell cytoplasm via the Type III secretion system (T3SS) apparatus. The invasion plasmid antigen Hs (IpaHs) are a novel family of bacterial E3 ubiquitin ligases that are secreted by Shigella, Salmonella, and Yersinia. …
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Regulation of BubR1 by Ultraviolet Radiation: Implications in Skin Cancer
… F-box) complex, belonging to the Cullin RING E3 ubiquitin ligase (CRL) family of E3 ubiquitin ligases that regulates the stability of a wide range of cellular proteins by recognizing and binding specific substrates through a conserved degron motif and targeting them for ubiquitination and …
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Genetic interactors of the SWI/SNF chromatin remodelling complex in Caenorhabditis elegans
… a loss-of-function mutation in either of the E3 ubiquitin ligases ubr-5 (UBR5 in humans) or hecd-1 (HECTD1 in humans) can restore development to adulthood in swsn-1 loss-of-function mutants that otherwise die as embryos. Furthermore, I showed that SWI/SNF protein levels are reduced in swsn-1; …
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Quality control of protein complex assembly
… and found several proteins related to the ubiquitin-proteasome system. Using fluorescent reporters of PSMB subunit degradation in cells depleted of individual candidates, I found that the E3 ubiquitin ligases KCMF1 and UBR4 are required for efficient degradation of unassembled PSMB …
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The Role of Two Homologous E3 Ligases In Muscle Physiology
<p>Ubiquitin-mediated proteasomal degradation is an essential cellular function that is coordinated by three key components: E1 ubiquitin activating enzyme, E2 ubiquitin conjugating enzyme, and E3 ubiquitin ligases. There are an estimated 600 E3 ligases, some of which share high sequence homology; …
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