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Showing 1 to 17 of 17 for “"Ubc9"”.
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The Role Of Ubc9 In Drug Resistance And Its Expression Regulation In Cancer Cells
… essential E2 conjugating enzyme for sumoylation, Ubc9 plays a central role in sumoylation-mediated cellular pathways. In this study, we investigate the role of Ubc9 in drug resistance as well as its regulation in cancer cells. An early gene product, Gam1, encoded by the avian adenovirus CELO, is …
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Effects of altered expression of the sumo conjugating enzyme, UBC9 on mitosis, meiosis and conjugation in Tetrahymena thermophila
… modification in eukaryotic species. Ubc9p is the E2-conjugating enzyme for SUMOylation and consequently it influences multiple cellular pathways. Nuclear proteins are common targets of SUMOylation and regulate nuclear events such as transcription, DNA repair and mitosis. The …
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Novel Insights into Ubiquitin-like Protein E1-E2 Interactions
… Sumo's C-terminus, binds the dedicated E2 enzyme Ubc9, and promotes Sumo C-terminal transfer between the Uba2 and Ubc9 catalytic cysteines. In this study we determined crystal structures of the C‑terminal ubiquitin-fold domain (ufd) from yeast Uba2 (Uba2<sup>ufd</sup>), alone and in complex with …
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Sumo and Ubiquitin Ligases Regulate Hepatocyte Nuclear Factor - 1 Beta Transcriptional Activity
… that interact with HNF-1β The SUMO E2 ligase, Ubc9, SUMO E3 ligases, PIAS1 and PIASgamma , and the ubiquitin E3 ligase, Arkadia, were isolated as putative interacting proteins. SUMOylation of transcription factors affects their subcellular localization, intracellular transport, stability, and …
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Characterizing the RanGAP1-RanBP2 complex in mitosis
… ausübt, zum anderen besitzt RanBP2 zusammen mit Ubc9 Sumo-konjugierende Aktivität. Gemeinsam sind diese Proteine essentielle Regulatoren des nukleo-zytoplasmatischen Transportes in Interphasezellen, und spielen des Weiteren eine wichtige, bislang jedoch kaum verstandene Rolle für die Funktion der …
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A phylogenetic study of ubiquitin conjugating enzymes and structural analysis of human UBE2J proteins
… solved structures for human UBE2J2 and human UBC9 were carried out. Significant differences were observed near the active sites of human UBE2J proteins compared to the active site for human UBC9.
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The Role of Esc2 and DNA in Smc5/6-mediated Sgs1-Top3-Rmi1 Sumoylation
… mediated by its SLD2 domain that interacts with Ubc9, and that this interaction is essential in the dissolution of HJ and genome stability. Therefore, we suggest that Esc2 is a SUMO E2 cofactor that facilitates the sumoylation of Mms21 substrate STR in HJ dissolution. In addition, we found that …
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Untersuchungen zur Funktion des RanGAP1 Proteins
… weder die Stabilität des RanBP2-RanGAP1*SUMO1-Ubc9-Komplexes noch die aktivierenden Eigenschaften von RanGAP1 direkt beeinflusst. Die Ergebnisse dieses Projektes flossen in die Veröffentlichung Swaminathan, Kiendl et al. (2004) J Cell Biol ein.
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Characterisation of Small Ubiquitin-like Modifier (SUMO) protein complex expression in human cancer cells to identify novel therapeutic targets
… expression of PIAS4 in NMR compared to SENP1 and Ubc9. SUMO machinery expression levels from NMR are recapitulated in cancer cells either by silencing (hUBE2I: shRNA, hSENP1: shRNA) or overexpression (Myc/hPIAS4: Exp) to study functional effects. Cytotoxicity assays were analysed in relevance to …
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RAD6-abhängige DNA-Reparatur wird durch Ubiquitin- und SUMO-Modifikation von PCNA reguliert
… neben PCNA auch das SUMO-konjugierende Enzym UBC9 mit den beiden RING-Finger Proteinen sowie mit PCNA selbst und ist so ebenfalls mit dem RAD6 -Reparaturweg assoziiert.Nach DNA-Schädigung wird PCNA von RAD6/RAD18 monoubiquitiniert.Alternativ kann PCNA durch das hierfür zusätzlich notwendige …
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Biochemical investigation of phosphodiesterase type IV post-translational modification, cellular localisation and interaction with associated binding proteins
… binding partners like β-Arrestin, AKAP18 δ and UBC9. Rolipram is an archetypal PDE4 specific inhibitor. In this study it is shown that in cells expressing a GFP tagged form of PDE4A4 undergoes redistribution into accretion foci upon chronic treatment with rolipram. Data suggests that foci …
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Development and Application of Mass Spectrometry Based Methodologies to Study Ubiquitin-Like Modifiers
… SUMOylation at 16 different lysines by UBC9 despite lacking a consensus motif.<br/><br/>Finally, a quantitative proteomics workflow is presented that enriches whole proteome datasets by combining peptide identifications from unlabelled and SILAC proteomes. A software implementation, with …
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Amyloid-beta signaling in physiology and pathology: from astrocytes to SUMO
… via transduction of its conjugating enzyme, Ubc9, rescued Abeta-induced deficits in synaptic plasticity and memory. I further demonstrate that inhibition of SUMOylation can directly cause such deficits, similar to Abeta. Overall, the data establish SUMO as a novel regulator of synaptic …
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CDC45 Function Alters Cell Sensitivity to DNA Topoisomerase I Poisons
… essential products for DNA replication and <em>UBC9</em>, which encodes an E3 SUMO ligase.</p> <p>The <em>tah</em> mutant <em>cdc45-10</em> has a single amino acid substitution (G<sup>510</sup>R). These cells are hypersensitive to Top1T<sup>722</sup>A and transiently accumulate in early S-phase …
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Understanding T cells in type 1 diabetes: a role for c-Maf and characterization of intracellular signaling following engagement of transgenic Ly49A.
… interaction with the E2 conjugating enzyme Ubc9 and increases following T cell stimulation. In addition, c-Maf physically interacts with p65/RelA. This interaction is dependent on the DNA binding domain of c-Maf and phosphorylation of p65 at serine 536. In transfected cells, overexpression …
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Signalling pathway of FBXO7 and its role in hereditary Parkinsonism
… the Sumo E3 ligase complex RanBP2/ RanGAP1*Sumo1/Ubc9 complex.<br/>• In the differential enrichment of ubiquitylated protein species in SILAC labelled wild-type and homozygous R379G Fbxo7 KI MEFs, I have clearly identifies 2 highly conserved lysine residues, which are conserved amongst VDAC 1, 2, …
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THE PHD FINGER OF SP140: A STRUCTURAL AND FUNCTIONAL STUDY
… revealed binding of recombinant SUMO E2 ligase Ubc9 to a small, well defined surface of Sp140 PHD finger. We next expressed and purified in E.coli Sp140 PHD finger – bromodomain (PB) tandem and through in vitro SUMOylation test and mass spectrometry analysis we found that the construct was …