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Showing 1 to 1 of 1 for “"UV melting curves"”.

  1. Potential prebiotic roles of (amino-)acylation in the synthesis and function of RNA

    … four sites of 2'-O-acetylation were assessed by UV melting curve analysis. Remarkably, the acetyl groups caused a consistent decrease in Tm of between 3.0-3.2 °C. Thermodynamic parameters indicated a decrease in duplex stability that was consistent with a decrease in hydration of the minor groove …

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