Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 41 for “"UV detection."”.
-
Electric Field Gradient Focusing-UV Detection for Protein Analysis
… EFGF device. Using fiber-based EFGF with UV detection, a concentration factor as high as 15,000 and a concentration limit of detection as low as 30 pM were achieved using bovine serum albumin as a model protein. I also demonstrated the potential of using fiber-based EFGF for quantitative …
-
Rapid analysis of inorganic and organic anions in kraft pulping liquors by capillary zone electrophoresis
… hydroxide-based electrolytes with indirect UV-detection. In the second scheme, polysulphide was speciated, and sulphur species such as sulphide, thiosulphate and sulphite, as well as hydroxide were separated and determined using a sodium sulphate, tetradecyltrimethylammonium hydroxide-based …
-
Development of micro-scale and automated membrane extraction systems for water analysis
… has been preiously published in the literature. Detection limits were at low to ppt level with RSDs between 1.60 and 7.65%. Large linear dynamic ranges with good linearity (R^2 between 0.9870 and 0.9997) were achieved. The implementation of a barrier film represents a major development in the …
-
Preparation, characterisation and modification of porous carbon monolithic materials for chromatographic and electrochemical applications
… 100% hexane (or pentane), flow rate 0.7 ml/min, UV detection 270 nm. The backpressure for the monolithic phase was 2 bar. Also, preliminary separations of mixtures of hydrophilic molecules (caffeine, theophylline and benzoic acid) using various concentrations of acetonitrile as a mobile phase …
-
Determination of Lactose by Reversed-Phase High Performance Liquid Chromatography.
… was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300-360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile …
-
Separation and Detection of 2,3-Dihydroxybenzoic Acid
… zone electrophoresis (CZE) coupled with UV detection to establish optimum separation conditions. These conditions were applied for rapid, efficient, and sensitive determination of 2,3-DHBA by CZE coupled with electrochemical detection. Quick and sensitive detection of 2,3-DHBA is …
-
Feasibility of analysis of polar compounds by high performance liquid chromatography with Fourier transform infrared spectroscopic detection
… cell Fourier transform infrared spectroscopic detection were developed for polar compounds including phenols, alcohols, amines and azaarenes. Detection by FTIR gave information concerning hydrogen bonding and solvent effects occurring during the separations in addition to giving structural …
-
HPLC method development for the evaluation of pheromones from the dwarf African clawed frog Hymenochirus
… of the phase modifier, wavelength for UV-detection, composition of the mobile phase, flow rate, and gradient elution were studied to achieve the required separation. The optimal method was determined as: direct sample loading, 0.01% (v v) formic acid in in mobile phase A (water) and …
-
High-performance liquid chromatographic methods for quantitative assessment of degradation products and extractives in pretreated lignocellulose.
… A reversed-phase HPLC method with UV detection is developed for simultaneous separation and quantitation of organic acids and neutral degradation products present in the corn stover hydrolysate. On the other hand, inorganic ions and some organic anions which present in water …
-
Electrochemical Detection of Aliphatic Sulfur Compounds in Liquid Coal Extracts
… using reverse phase HPLC with electrochemical detection in a acetonitrile/water mobile phase. Coal samples IBC 101 and IBC 105 were extracted with THF and hexane. The liquid coal extracts were fractionated using a simple chromatographic technique. The fractionated extracts were then analyzed …
-
Velocity-difference induced focusing in capillary electrophoresis and preparative capillary electrophoresis
… was then applied to serotonin to improve the detection limit when CE was used with a UV absorption detector. It was also applied to focus amino acids, peptides, and proteins to improve the concentration sensitivity. It is found that the pKa rather than the pI of the analytes is the key …
-
Development of a HPLC method for the determination of methotrexate (MTX) and MTX-polyglutamates of red blood cells: A dissertation submitted in partial fulfilment of the requirements for the Degree of Bachelor of Science with Honours at Lincoln University
… reversed-phase column, followed by ultraviolet (UV) detection at 254 nm. RBC standards for MTX and MTX(glu)s were linear over the concentration range of 0 - 500 ng/200 μI. The minimum detection limit of MTX and MTX(glu)s was 15.6 ng/200 μI. MTX, MTX(glu)2 & MTX(glu)3 were detected in the RBCs of …
-
Pressure-driven and electroosmotically-driven liquid chromatographic separations in packed fused silica capillaries
… this case, a suppressor column is not used and UV detection is employed in the analysis of nucleoside monophosphates. Again, column performance characteristics are measured and noted. Application of this system to the separation of a hydrolysed nucleic acid sample demonstrates the feasibility of …
-
Selectivity and detection in capillary electrophoresis
… the minimization of some of the selectivity and detection limitations in capillary electrophoresis. A more practical design of an electrochemical detector is introduced with simultaneous on-line UV detection¹, for the selective detection of a number of pungent and neurological compounds, the …
-
Development and Bioavailability/Bioequivalence of a Fixed-Dose Combination Antiretroviral Reconstitutable Suspension Intended for Pediatrics
… from the subjects were analyzed by HPLC with UV detection. Non-compartmental pharmacokinetic analysis was performed to obtain the Cmax, AUCo-t, and AUCo-inf utilizing WinNonlin software. Analysis of variance of the formulation, period, sequence, and subject effects established the absence of …
-
Rapid separation of biologically important low molecular weight compounds by microchip electrophoresis and ultra-fast performance liquid chromatography
… An overview of the history, different modes and detection techniques coupled to CE is provided. The advantages of microchip electrophoresis are highlighted. Some aspects of metal complex analysis by capillary electrophoresis are described. Finally, the theory and different modes of the liquid …
-
Food safety in wine: optimization of analytical controls and evaluation of production technologies
… chloride (FMOC-Cl) and UV detection was developed. Initially, the influence of pH, time of derivatization, gradient profile were studied. In order to improve the separation of amino acids and amines and reduce the time of analysis, it was decided to study the influence of …
-
Measurement of Phytase Activity in a Clymer Forest Soil Using the TInsP5 Probe
… liquid chromatography (RPHPLC) with UV detection. PA was calculated based on a mass balance approach. A soil dilution technique was developed to address the challenge of determining maximal PA in soils containing higher organic matter content. In the initial report on use of the …
-
Analysis of Alcohol and Alkylphenol Polyethers via Packed Column Supercritical Fluid Chromatography
… aryl group present in APEOs allowed ultraviolet (UV) detection, with an equal molar response for oligomers, allowing average molar oligomer values to be calculated. APEOs were separated by ethoxylate unit via SFC-UV as well as normal phase HPLC-UV employing packed columns. Stationary phase and …
-
Detection of Proteins by Two-Photon Excitation of Native Fluorescence
… migration in an opposing hydrodynamic flow. The detection principles that are compatible with µTAS separation may not always scale down. This thesis represents the development of laser-induced two-photon fluorescence detection on a microchip separation device. This detection is based on …
Page 1 of 3