Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 22 for “"UV crosslinking"”.
-
Development of in vitro iCLIP techniques to study spliceosome remodelling by RNA helicases
… on a defined RNA substrate are analysed by UV crosslinking and next-generation sequencing, which allows for the determination of the RNA helicase binding profile at nucleotide resolution. In vitro spliceosome iCLIP (individual-nucleotide resolution UV crosslinking and immunoprecipitation) …
-
Evidence that ARNT plays a role in the regulation of the immunoglobulin heavy chain enhancer and identification of a putative ARNT ligand
… This ligand was partially characterized by UV crosslinking studies and a variety of biochemical studies using electrophoretic mobility-shift assays. Preliminary data suggests that it is hydrophilic, heat-stable, small, and non-protein.</p>
-
Apobec-1 Complementation Factor (ACF) Binds and Regulates Multiple RNAs
… 1 and 2 together bind at least some RNA targets. UV-crosslinking assays identified discrete ACF binding sites within both the IL-6 3'UTR and the Cox-2 3'UTR. On both RNAs, these sites are consistent with regions that confer ACF-dependent mRNA stabilization or destabilization in RNA turnover …
-
Engineering a microneedle patch for opioid overdose therapy
… 500 µm pitch) were fabricated with tunable UV crosslinking (0s, 15s, 30s, 60s) to optimize their physicochemical properties. Results indicated that 60s-crosslinked GelMA MNs exhibited enhanced mechanical strength, reduced enzymatic degradation (34.03% at 360 min), and a prolonged drug …
-
Fundamentals and application of the collapse transition of poly(acrylic acid) for synthesis of various polymer-templated nanoparticles
… Upon stabilization of the nanoparticle via UV crosslinking, further chemical reaction of the counterions can be performed. In principle, the method can be carried out with different polyelectrolyte-counterions pair, and hence has enormous potential for generating different kinds of …
-
Aspekte der Translationskontrolle in der Drosophila-Spermatogenese: Charakterisierung regulatorischer Elemente
… einem terminalen und zwei internen loops aus. In UV-Crosslinking-Experimenten bindet ein einzelnes 62 kDa Protein innerhalb der stem-loop Struktur. Eine hohe Sequenzähnlichkeit dieser Region besteht mit dem TCE ("translational control element") der Drosophila Mst(3)CGP-Genfamilie, welches …
-
Investigating the mechanism of RNA packaging in Rift Valley Fever phlebovirus
… with viral RNPs in infected cells. Furthermore, UV-crosslinking and immunoprecipitation analysis showed that Gn also directly binds to genomic and antigenomic viral RNAs, revealing the presence of a direct interaction between Gn and viral RNAs in infected cells for the first time in bunyaviruses. …
-
Characterization of the Association of mRNA Export Factor Yra1 with the C-terminal Domain of RNA Polymerase II in vivo and in vitro
… Recognition Motif (RRM) (residues 77-184). Using UV crosslinking, I found that the RRM alone can bind RNA, although a larger protein segment, extending to the C-terminus (aa 77-226), displays stronger RNA binding activity. Even though the RRM is implicated in both RNA and CTD binding, certain RRM …
-
The Role of Secondary and Tertiary Structure in the Cap-Independent Translation of FGF-9 and HIF-1-alpha
… toeprinting, DAP5 SHAPE footprinting, and UV crosslinking were used to map the binding sites of DAP5 along the FGF-9 and HIF-1-alpha 5’ UTR RNAs. Lastly, I used bioinformatics software to propose a three-dimensional binding model of DAP5 to these RNAs. DAP5 appears to bind specific, but …
-
Identification of Loss of Specific FMRP-RNA Interactions as a Cause of Fragile X Syndrome
… mRNAs bound by FMRP in mouse brain, we have used UV crosslinking and immunoprecipitation (CLIP), a new methodology that has several advantages over conventional methods for identification of bona fide in vivo RNA ligands for RNA binding proteins. We obtained a defined set of FMRP RNA targets, …
-
Proteomic Approaches For Profiling Cofactor-Dependent Proteins In Mycobacterium Tuberculosis
… of PLP-dependent enzymes, a linear diazirine for UV-crosslinking, and an alkyne moiety to enable enrichment of crosslinked proteins. Our molecule was utilized to study PLP-dependent enzymes in vitro as well as look at whole cell lysates of Mycobacterium tuberculosis and assess inhibitory activity. …
-
Synthesis and Characterization of Poly(arylene ether sulfone)s for Reverse Osmosis Water Purification and Gas Separation Membranes
Crosslinking is an effective technique for increasing the salt rejection of water purification membranes and the selectivity of gas separation membranes. An abundance of monomers, telechelic oligomers, and novel polymers were synthesized for use as separation membranes. These materials were often …
-
Modulation of Ago-miRNA regulatory networks by cis-sequence elements and target competition
… all targets of miRNA regulation, we performed UV crosslinking and immunoprecipitation (CLIP) of Ago complexes in mouse embryonic (ESC) and mesenchymal (MSC) stem cell lines. We also captured the genome-wide miRNA-independent binding footprint of Ago by performing CLIP in cells that lack Dicer, …
-
Studies of Interactions Between Rod-like Celulose Nanocrystals and Xylan and Pullulan Derivatives: A Light Scattering Study
… nanocrystals (DOE-2-12B) was also studied by UV-Vis spectroscopy, zeta-potential measurements, and polarized and depolarized dynamic light scattering. UV-Vis spectroscopy of the P4CC03/water binary system and in situ light scattering showed UV crosslinking of pullulan 4-chlorocinnamate …
-
Molecular Mechanisms of Alternative Splicing Regulation: An Investigation of the Spliceosome Repressed by Hnrnp L on Cd45 Exon 4
… the skipping of CD45 exon 4. Using psoralen- and UV-crosslinking analysis, U1 snRNP and other protein components that associate with the sequences around the 5' ss within the hnRNP L-repressed spliceosome complexes were compared with the control complexes. These studies revealed that hnRNP L …
-
Identification and characterisation of non-coding RNAs involved in ductal carcinoma in situ
… oncogene. Using RNA-affinity chromatography and UV-crosslinking, followed by mass spectrometry, protein interactors of vtRNA2-1 were identified. These showed enrichment in cytoskeletal processes. Furthermore, I examined genes with altered expression following knockdown of vtRNA2-1, and many of …
-
Self assembled transmembrane protein polymer conjugates for the generation of nano thin membranes and micro compartments
… to act as the pore. The membranes were formed by crosslinking of transmembrane protein polymer conjugates. The conjugates were self assembled on air water interface and the polymer chains crosslinked using a UV crosslinkable comonomer to engender the membrane. The protein used for the studies …
-
Template-Directed Synthesis of One-Dimensional Hybrid Nanostructures from Cylindrical Polymer Brushes
… nanotubes from agglomeration and intermolecular crosslinking during the hybrid formation. Additionally, POEGMA rendered the hybrid nanomaterials soluble in various solvents, including water. Water-soluble core-shell CPBs were obtained from microphase separated cast films of …
-
Synthesis and Characterization of Toughened Thermally Rearranged Polymers, Poly(2,6-Dimethylphenylene-oxide) Based Copolymers and Polymer Blends for Gas Separation Membranes
… films, then crosslinked in the solid state with UV light. The ketone and benzylic methyl groups crosslinked upon exposure to UV light. For the study of PPO copolymers, copolymers were prepared by polycondensation of a difunctional PPO oligomer with 4,4’-difluorobenzophenone or …
-
Advanced Polymeric Membranes and Multi-Layered Films for Gas Separation and Capacitors
… bisphenol F can undergo oxidation reactions and crosslinking with UV light. The polymers were oxidized under two different conditions, one by chemical treatment using oxone and KBr and one by elevated thermal treatment in air. Thermogravimetric analysis, 1H-NMR and attenuated total reflectance …
Page 1 of 2