Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 3 of 3 for “"UBR4"”.
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Quality control of protein complex assembly
… I found that the E3 ubiquitin ligases KCMF1 and UBR4 are required for efficient degradation of unassembled PSMB subunits. Biochemical assays showed that KCMF1 and UBR4 form a stable complex that physically interacts with nascent PSMB subunits. Moreover, using a cell-based flow cytometry assay, I …
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The proteasome and its ancillary proteins
… investigated the proteasome interaction with the UBR4 E3 ligase. I optimised the preparation of 26S proteasomes and found UBR4 co-purifying. I also showed that endogenously-tagged UBR4 conversely co-purifies with proteasome complexes. My biochemical and biophysical experiments indicate a strong …
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Understanding Host-Pathogen Interactions of Rift Valley Fever Virus That Contribute to Viral Replication
… Gn and L proteins that aid in viral replication. UBR4 was determined to be an interactor of Gn via immunoprecipitation followed by either LC/MS/MS or western blot analysis. Its inhibition via siRNA or CRISPR-Cas9 knockout showed a reduction of viral titers and viral RNA production. It was …