Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"U937"”.
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Measuring the Changes in Tumor Necrosis Factor-Alpha (TNF-α) from Secretory Populations of U937 Monocytic Cells during Differentiation.
… (PMA) on the induction of TNF-α secretion from U937 monocytic cell populations by using the reverse hemolytic plaque assay (RHPA). The RHPA will allow us to investigate both changes in TNF-α secreting populations as well as monitor the relative amount of TNF-α released from individual cells. Our …
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Human macrophage model for selective evaluation of CD8⁺ and γδ⁺ cytotoxic T cell function in tuberculosis
The human macrophage cell line U937 was investigated as an in vitro model for human macrophage function in mycobacterial infections. This involved evaluating the ability of differentiated U937 cells to phagocytose Mycobacterium tuberculosis, control intracellular mycobacterial growth, and present …
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Mammalian cell cultures as a model system for the determination of cytotoxicity induced by cholesterol oxidation products
… cell lines, HepG2, a hepatoma cell line, and U937, a monocytic cell line. Both 25-OH and 7β-OH were cytotoxic to the HepG2 cell line but apoptotic cells were not detected and it was concluded that cells underwent necrosis. 25-OH was not cytotoxic to the U937 cell line but it was found to have …
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Genetic factors that determine host resistance to bacterial infection
… enterica serovar typhimurium infection of U937 cells and examines the role the gene SLC7A11 plays in the infection process a previous work identified that SLC7A11 gene mutations decreased susceptibility to infection. SLC7A11 CRISPR #1 U937 cells were infected with Salmonella enterica …
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Proto-oncogene c-jun expression is induced by AML1-ETO in a JNK dependent manner:possible role in the pathogenesis of acute myeloid leukemia
… and c-jun mRNA expression levels. In myeloid U937 cells, c-jun mRNA and c-Jun protein expression levels increased upon induction of AML1-ETO. AML1-ETO transactivated the human c-jun promoter through the proximal AP-1 site via activating the JNK signaling pathway. JNK targets c-Jun and ATF-2, …
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Effects of Linoleic Acid on Tether Formation between Monocytes and Endothelial Cells
… and endothelial cells. </p><p>Treatment of U937 monocytes with free linoleic acid or albumin-bound linoleic acid reduced the cortical tension of the monocytes. The effects of albumin-bound linoleic acid on the membrane were governed by the exchange of linoleic acid from albumin to the …
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Effect of Cigarette Smoke Exposure on Bronchial Hyperresponsiveness in Mice and Response to a Corticosteroid and/or a β2-Adrenergic Agonist and Effect of Cigarette Smoke Extract on Cytokines Release from U937 Cells.
… and IL-10) release from macrophage like cells (U937 cell line).|We found that cigarette smoke exposure in mice increased BHR to MCh. Also, the BHR in smoke exposed mice treated with FP and/or salmeterol was significantly higher than BHR in non-smoked mice treated with the same drugs. The drugs …
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In Situ Regulation of Cytosolic Phospolipase A₂
… differentiation of the human lymphoma cell line, U937, induced an enhanced capacity to mobilize arachidonate in response to the calcium ionophore A23187. The arachidonate mobilizing activity in differentiated cells was consistent with characteristics reported for cPLA<sub>2</sub> in<em> …
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Biomarkers of oxidative stress in models of schizophrenia.
… neuroblastoma IMR-32 cell line and the lymphoma U937 cell line. For the three cell lines, the study assessed the cytoprotective effects of phenolic antioxidants and essential fatty acids in affording protection to cellular DNA, protein and lipids from an oxidative challenge. In addition, two …
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C-Rel Is A Transcriptional Target of Mesoderm Inducer In Xenopus Like 1 (Mixl1)
… in the human myelomonocytic leukemia cell line, U937, I performed global chromatin immunoprecipitation coupled sequencing (ChIP-Seq), expression profiling studies I identified several putative targets. Of these, the proto-oncogene c-REL was an important target. Both ectopically expressed and …
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Role of nuclear factor kappa B (NFkB) in the vascular complications of diabetes.
… followed by sequencing of the product. The U937 monocyte cell line was used to investigate the expression of the NFĸB gene. Cells were incubated in RPMI 1640 with 2 mM L-glutamine, 100 U/mL Penicillin, Streptomycin and 10% heat inactivated foetal bovine serum (FBS) with H2O2 for up to 180 …
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Role of cytochrome P450 enzymes on alcohol/nicotine-mediated oxidative stress and cytotoxicity in monocytes/astrocytes: Implications for HIV-infected alcohol/tobacco users
… CYP2E1 and CYP3A4 are induced by alcohol in U937 monocytes/macrophages, while only CYP2E1 is induced by alcohol at both mRNA and protein levels in SVGA astrocytes, 2) CYP2A6 metabolizes nicotine in U937 macrophages and SVGA astrocytes, leading to increased production of ROS, 3) CYP3A4 binds …
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OmpD but not OmpC is involved in adherence of Salmonella enterica serovar Typhimurium to human cells
… the role of OmpC and OmpD in adherence to human U937 macrophages and T84 intestinal epithelial cells. OmpC and ompD mutant strains were created by transposon mutagenesis utilizing P22-mediated transduction of Tn10 and Tn 5 insertions into wild-type strain 14028. Fluorescein-labeled wild-type and …
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The synthesis, characterization, and use of a protein-cysteine proteinase inhibitor complex for the study of endosome/lysosome fusion
… presence in human cell monocytic lines THP1 and U937 was detected by the use of the membrane-permeable, irreversible, active-site directed inhibitor Fmoc-(¹²⁵I)Tyr- Ala-CHN₂ followed by immunoprecipitation of the enzymes, SDSPAGE, and autoradiography. All three enzymes were detected in THP1 …
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Development and Characterization of A Bi-Specific Antibody Targeting Hla-A2/Pr1
… elimination of the leukemia cell line U937 (as evidenced by % leukemia cells detected in peripheral blood and bio-luminescence imaging) in an NSG-U937 AML xenograft mouse model when compared to control groups treated with effector cells alone. In conclusion, these studies demonstrate …
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New Types of Morpho-Physiological Changes in Cells Exposed to Nanosecond Pulsed Electric Field
… show that microvesiculation develops in HL60 and U937 cells in response to Ca<sup>2+</sup> presence during nsPEF exposure. Microvesiculation does not depend on colloid-osmotic swelling (COS). PLBs are produced in U937 cells due to active formation of actin cortex and require the absence of …
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Autonome Parvoviren
… and apoptotic cell death of the human monocytic U937 cell, in a way similar to the apoptotic cell death induced by TNFα. Moreover, the c-myc gene, overexpressed in these cells, is down-regulated during H1 infection. In agreement with these data, we were able to show -by comparing the gene …
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Insulin resistance in cancer cachexia and metabolic syndrome
… address this premise, firstly, human cell line U937 differentiated macrophages were exposed to insulin, LPS and PGE2. In these cells, insulin significantly augmented the gene expression of the pro-inflammatory mediators IL-1β, IL-8, CCL2, Oncostatin M (OSM) and microsomal prostaglandin E2 …
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Differenziamento macrofagico: gli effetti dei polimeri sintetici
… different stages of macrophagic differentiation: U937 pro-monocytes, THP-1 monocytes and RAW 264.7 macrophages. Cytotoxicity was evaluated by measuring the rate of viability with MTT, Neutral Red and morphological analysis at light microscope in time-course dependent experiments. The influence of …
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