Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"U87MG"”.
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Expression of inflammasome-related molecules from U87MG cells cocultured with Naegleria fowleri in the non-contact system
… N. fowleri trophozoites in a non-contact system, U87MG cells were cultured with TRAIL, LPS and amoebic trohozoites for 30, 60 and 180 mins, respectively. By Western blotting and ELISA analysis, amounts of IL-1β and NLRP3 protein from U87MG cells cocultured with N. fowleri trophozoites were …
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Involvement of CD133+ stem cells in the chemoresistance of glioma
… of this disease. In the current study, the U87MG and 1321N1 glioma cell lines were cultured in serum containing medium (SCM) and serum free medium (SFM). In SFM, the U87MG cells grew as neurospheres suggesting the presence of stem cells in this cell line while in the 1321N1 cells no …
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Effects of Thyroid hormone on Cardiomyocytes and on Glioma differentiation and proliferation
… 1321N1 cell line, an astrocytoma grade II, and U87MG, a glioblastoma grade IV. Cells were exposed for 2 and 4 days in culture medium deprived of T3 (non-treated cells) and in a medium containing either 1 nM T3 (at near physiological range) or 500 nM T3 (supraphysiological). From the initial …
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Selective targeting to glioma with nucleic acid aptamers
… showed strong binding affinity to the target U87MG cells as measured by flow cytometry. SA44 and SA43 showed higher uptake and cytoplasmic localisation in U87MG and 1321N1 glioma cell lines compared to non-cancerous SVGP12 cells and non-glioma MCF-7 and T24 cells as measured by confocal …
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Targeting of adenovirus serotype 5 infectivity to EGFRvIII-expressing cells using a bispecific CAR-scFv.
… and EGFRvIII, respectively. Additionally, a U87MG EGFRvIII transduced cell line, as opposed to its parental U87MG cell line, showed increased infectivity by adenovirus when incubated with CAR/MR1. These findings suggest that CAR/MR1 is able to bind to and re-direct infection of adenovirus to …
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Suppression and activation of lipid signalling in cancer : tumour suppressor PTEN and oncogenic sphingosine kinase
… were transiently transfected into: (A) PTEN null U87MG glioblastoma cells, and WT PTEN expressing (A) HCT116 colon cancer and (C) MCF7 breast cancer cells. Transfected cells were then assayed for cell proliferation, cell cycle phase distribution and AKT phosphorylation. In U87MG cells, 50% of the …
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The Generation of Novel Aptamers for Glioma and their Pharmacokinetic Profile In Vitro
… aptamers against the grade IV glioma cell line U87MG with counter selection carried out against the non-cancerous foetal astrocyte cell line SVGp12. Optimisation of the SELEX protocol involved the adjustment of PCR parameters, selection cell numbers and identification of ideal cloning plasmid. …
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Halting the neural progression of cancer
… repulsion was observed in PC12 and U87MG cells, which express NRP2. SEMA3A also successfully repelled SY5Y cells, which express NRP1. Neurite length was significantly inhibited by SEMA3F in PC12 and U87MG neurite outgrowth assays. Taken together, our results suggest that SEMA3F is a …
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Diagnostic Neuropathology of Brain Tumours using Biophotonics and Spectrometry
… of brain tumours using two tumour cell lines, U87MG, 1321N1 and a control, SVGP12. Another study looked at two approaches, elemental profiling of both tissue and serum using inductively coupled plasma-mass spectrometry. Trace elements increase or deficiency has been linked to cancer development …
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Determining the in vitro anti-cancer effects of various novel indoles and an anti-microbial peptide towards a potential treatment of glioma.
… of indoles against glioma cell lines (1321N1 and U87MG) using a number of different cell-based assays and also to compare them with conventional anti-cancer drugs. The aim was to find potent anti-cancer compound(s), amongst the compounds tested, and by studying its preliminary …
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Μεθοδολογίες μοριακής απεικόνισης με επισημασμένα νανοσωματίδια για τον ποσοτικό προσδιορισμό της χωροχρονικής κατανομής της αγγειογένεσης σε καρκινικούς όγκους
… πρότυπα καρκίνου τύπου γλοιοβλαστώματος U87MG. Η αξιολόγηση της χωροχρονικής κατανομής της αγγειογένεσης κατέδειξε μέγιστη στόχευση στις ιντεγκρίνες ανβ3 σε ποσοστό 11,60±3,05 % ID/g για το παράγωγο 99mTc-RGD και 9,01±0,19 ID/g για τα στοχευμένα νανοσωματίδια 99mTc-NPs-RGD. Το 99mTc-RGD …
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The neuronal RNA binding protein HuB as a potential tumor suppressor in glioblastoma
… progression. This hypothesis was tested using U87MG cells, a commonly used glioma cell line homozygously deleted for CDKN2A and heterozygously deleted for ELAVL2 and primary glioma initiating cells (GICs) homozygously deleted for both genes. Migration, invasion and capacity to form nuerospheres …
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The Expression and Function of Wilms' Tumor 1 in Malignant Glioma
… from p53-mediated cell death. We then generated U87MG cells (p53 wild-type) that stably expressed WT1 to model an endogenous p53 response. It is well known that after treatment with ionizing radiation, U87MG cells readily undergo p53-mediated apoptosis. Again, WTI expression did not protect …
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EXAMINATION OF CD133 AND CD147 AS CANCER STEM CELL MARKERS
… which shows that formation of neurospheres with U87MG glioblastoma cells may be driven by increased expression of CD147, correlated with increased CD133 expression. These findings suggest that relative levels of CD147 expression may be used as a determinant to target cancer stem cells in …
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Structually diverse Cu-64-labeled RGD peptide conjugates for PET imaging of αvβ3 expression
… expressing cell line, U87MG human glioblastoma cells, was determined. The compounds were screened via biodistribution studies and microPET imaging of a murine U87MG tumor model. Polyvalence has known to have a profound effect on receptor-binding affinity and <italic>in …
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A constraint optimization framework for discovery of cellular signaling and regulatory networks
… sequencing and mRNA profiling data from the U87MG glioblastoma cell lines over-expressing the variant III mutant of the epidermal growth factor receptor (EGFRvIII). In both cases the method identifies changes in diverse cellular processes that extend far beyond the expected pathways. Analysis …
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Investigation of Psychotropic Drugs for the Treatment of Glioblastoma
… Drug efficacy was initially determined in U87MG and T98G cell lines using viability and clonogenic assays. These demonstrated lower IC50 and LD50 values for the psychotropic drugs and statins compared to temozolomide. These effects were enhanced when the psychotropic drugs were used in …
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Probing the Redox and Photophysical Properties of Ru(II)-Pt(II) Supramolecular Complexes as Efficient Photodynamic Therapy Agents
… were studied in the presence of DNA and U87MG cancer cells. Thermal binding at the cis-PtCl₂ BAS in the Ru(II)-Pt(II) architecture was compared to cisplatin displaying similar reduced migration through the gel attributed to covalent binding to DNA. DNA photocleavage studies provided …
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The Identification and Validation of Novel Aptamers to Glioma
… Previously, the glioblastoma cell line U87MG has been utilised in cell-SELEX for aptamer identification, however cell lines may not ubiquitously express all biomarkers indicative of glioblastoma. Consequently, cell-SELEX using short term cultures from human tissue may produce aptamers …
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