Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"U6 snRNA"”.
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On co-transcriptional splicing and U6 snRNA biogenesis
… (snRNPs). They consist of one of the U-rich snRNAs U1, U2, U4, U5 or U6 together with several particle-specific proteins and core proteins. All mRNA processing events can occur co-transcriptionally, i.e. while the RNA is still attached to the gene via Pol II. The in vivo studies of …
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Conformational features of the human U2-U6 snRNA complex
… of the process. A complex formed between U2 and U6 snRNAs is implicated in the chemistry of pre-mRNA splicing. The catalytic activity of the U2-U6 snRNA complex is dependent on the presence of Mg2+ ions, and the complex has been shown to have several specifically bound Mg2+ binding sites in …
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Conformation of the U12-U6atac snRNA Complex of the Minor Spliceosome and Binding by NTC-related Protein RBM22
… (sn)RNA-protein complexes (U1, U2, U4, U5 and U6) and >100 proteins. snRNA components catalyze the two transesterification reactions while proteins perform critical roles in assembly and rearrangement. U2 and U6 snRNAs are the only snRNAs directly implicated in catalyzing the splicing of …
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Electron microscopic localization of tagged proteins in the yeast S. cerevisiae spliceosomal U4/U6.U5 trisnRNP
… den sogenannten U1, U2, U5 und U4/U6 snRNPs sowie zahlreichen nicht-snRNP Spleißfaktoren assembliert. Die snRNPs bestehen aus einem RNA-Molekül (bzw. zwei weitgehend basengepaarten RNAs im Fall von U4/U6) und mehreren Proteinkomponenten. Die Assemblierung des Spleißosoms beginnt mit …
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Structural studies of spliceosome assembly and catalysis
… catalytically active spliceosome, in which the snRNA components of the snRNPs mediate intron recognition and catalysis of splicing. Despite the importance of splicing to fundamental eukaryotic molecular biology, and the contribution alternative splicing makes to organismal diversity and disease …
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Quantitative modeling of 5' splice site subclass regulation and evolution
… subclass regulation involving METTL16- mediated U6 snRNA modification and reconcile both models by devising computational tools that identify sequence features predictive splicing dysregulation in transcriptome-wide datasets. Finally, I examine the evolutionary dynamics of left- and right-handed …
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Kristallstrukturanalyse des spleißosomalen DEAD-Box Proteins hPrp28
… Es ist notwendig für die Integration des U4/U6.U5 tri-snRNP in diesen makromolekularen Komplex und die anschließend stattfindende Konformationsänderung, die zum aktiven Spleißosom und zur ersten Transester-Reaktion führt. Vermutlich katalysiert hPrp28 dabei an der 5 -Spleißstelle der prä-mRNA …
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STRUCTURAL INSIGHTS INTO RNA POLYMERASE III PRE-INITIATION COMPLEX AND THE PIONEER ROLE OF OCT1
… III (Pol III) is selectively recruited to snRNA promoters remain poorly understood. This is particularly notable because Pol II- and Pol III-transcribed snRNA promoters share highly similar architectures and rely on the same core transcription factor, SNAPc. How a single complex enables …
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Development of in vitro iCLIP techniques to study spliceosome remodelling by RNA helicases
… to activate the helicase Brr2 to unwind the U4/U6 snRNA duplex, which ultimately leads to the formation of the spliceosome active site. To explore the role of Snu114, a complex built from Snu114 and a part of Prp8 was expressed and analysed in its natural context, bound to U5 snRNA. However, …
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A Cell Biological Determination of Integrator Subunit Localization
<p>Uridine-rich small nuclear (U snRNAs), with the exception of the U6 snRNA, are RNA polymerase II (RNAPII) transcripts. The mechanism of 3’ cleavage of snRNAs has been unknown until recently. This area was greatly advanced when 12 of the Integrator complex subunits (IntS) were purified in 2005 …
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Characterization of splicing mechanisms by single-molecule fluorescence
… pre-mRNA splicing. Out of five snRNAs, only the highly conserved U2 and U6 snRNAs are required in both steps of RNA splicing. The U2-U6 snRNA complex forms the active site of the spliceosome and has been shown to undergo splicing-related catalysis in the absence of proteins. …
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Investigation of Protein-protein Interactions within the Human Spliceosomal U4/U6.U5 tri-snRNP Particle
… Proteinen des U5, wie auch des U4/U6 snRNPs. Es kann daher als potentiell brückenbildendes oder vernetzendes Protein bei der Formation oder der strukturellen Stabilisierung des tri-snRNPs gesehen werden. Die stabile Integration des 102K Proteins in das U5 snRNP wird über …
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Efforts to elucidate functional and regulatory aspects of gene expression in Trichomonas vaginalis
… strong RNA polymerase (Pol) III promoter of the U6 snRNA gene in T. vaginalis exhibits a similar structural organisation to the orthologues in metazoans and can be used to produce sequence-customisable non-coding RNAs in this protist. This finding contributed to the successful deployment of the …