Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"U1A"”.
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Investigation of the dynamics and conformational changes of the U1A protein
The U1A-SL2 RNA complex is a model system for studying interactions between RNA and the RNA recognition motif (RRM), which is one of the most common RNA binding domains. Despite extensive studies conducted to study the origins of U1A-RNA affinity and specificity, little is known about the kinetics …
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NMR Studies of the RNA Binding Domain of U1A protein and its Complexes with RNA
… by five RNA protein complexes called snRNP's. U1A is one protein component of the U1 snRNP particle. The role of U1A in splicing is unclear, but it does bind a hairpin loop from U1 RNA. U1A also binds two internal bulges found in the 3' untranslated region of the mRNA which encodes the U1A …
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Folding Thermodynamic and Kinetic Characterization of Small Model Proteins: Lambda Repressor, U1A, Trpzip2 and PAO -12
Complete prediction of the folding processes for complex protein molecules through first principles is currently not possible (2003). Here we take an empirical approach towards a better understanding of the protein-folding problem, through thermodynamic and kinetic characterizations of several …
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Structure and expression of a U1 snRNA gene from Arabidopsis thaliana
… consisting of between five and eight U1 genes. U1a snRNA is unique in having an extra U residue at position +2, which occurs in the region of U1 RNA which base pairs with the 5' splice site of introns. Mutations in this region have been shown to affect splicing, implying the sequence …
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Exploring Protein -Rna Interactions With Site-Directed Mutagenesis and Phage Display
… The model systems we are investigating are U1A and CUG-BP1. In this dissertation, the molecular basis of the binding affinity of U1A-RNA beyond individual contacts was investigated. We have identified and evaluated the contributions of the local cooperativity formed by three neighboring …
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Exploring Protein-Rna Interactions With Site-Directed Mutagenesis and Phage Display
… The model systems we are investigating are U1A and CUG-BP1. In this dissertation, the molecular basis of the binding affinity of U1A-RNA beyond individual contacts was investigated. We have identified and evaluated the contributions of the local cooperativity formed by three neighboring …
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Exploring protein-RNA interactions with site-directed mutagenesis and phage display
… The model systems we are investigating are U1A and CUG-BP1. In this dissertation, the molecular basis of the binding affinity of U1A-RNA beyond individual contacts was investigated. We have identified and evaluated the contributions of the local cooperativity formed by three neighboring …
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Investigation of the interactions in RNA recognition motif-RNA complexes: small molecule inhibitors and kinetics of dissociation
… RNA recognition motifs and two RRM proteins, U1A and Sex lethal protein. Chapter 2 describes the identification and analysis of three small molecules that disrupt two different RRM-RNA complexes, Sex lethal protein-tra RNA and U1A-SL2 RNA. The research discussed in chapter 3 focus on the role …
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The contribution of construction materials to the airborne radon concentrations in an underground facility
… fiber crete and the native material from the U1a Complex at the Nevada Tests Site, Nye County, Nevada, were quantified and compared to each other. Gamma spectroscopy measurements resulted in an average 222Rn concentration to be 0.038 +/- 0.0011 and 0.014 +/- 0.001 Bq g-1 for the native …
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In silico protein evolution by intelligent design: creating new and improved protein structures
… proteins (human procarboxypeptidase and U1A) showed them to be virtually identical to the template natural counterparts.Second, we extended the capabilities of RosettaDesign to create a protein topology not observed in nature, by iterating between sequence design and structure prediction. …