Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"U1 snRNP"”.
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Co-transcriptional recruitment of the U1 snRNP
… genes. In my thesis, I address: 1) whether the U1 snRNP, which binds to the 5¡¦ splice site of each intron, is recruited co-transcriptionally in vivo and 2) if so, where along the length of active genes the U1 snRNP is concentrated. U1 snRNP accumulates on downstream positions of genes …
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Promoter directionality is controlled by U1 snRNP and polyadenylation signals in mouse embryonic stem cells
… find that an asymmetric distribution of encoded U1 snRNP binding sites (U1 sites or 5' splice sites) and PASs surrounding gene transcription start sites (TSSs) enforce promoter directionality by ensuring uaRNAs are prematurely terminated and likely subsequently degraded. Together, these studies …
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Purification and crystallization of spliceosomal snRNPs
… Spleißosoms sind die Ribonukleoproteinpartikel (snRNPs) U1, U2, U4, U5 und U6. Neben der U1 small nuclear RNA (snRNA) enthält das U1 snRNP drei partikel-spezifische Proteine, U1-A, U1-70k und U1-C. Des weiteren findet man einen Satz von sieben Proteinen, den Sm-Proteinen, die auch in anderen …
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Investigation of the higher order structure of the spliceosomal RNA network
… Über die räumliche Anordnung der beteiligten U1 und U2 snRNPs in frühen spleißosomalen Komplexen ist wenig bekannt. Beide snRNPs, zusammen mit weiteren nicht-snRNP Proteinen, sind in der korrekten Positionierung der für das Spleißen wichtigen funktionellen Bereiche der prä-mRNA involviert. Um …
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Molecular Mechanisms of Alternative Splicing Regulation: An Investigation of the Spliceosome Repressed by Hnrnp L on Cd45 Exon 4
… the spliceosome, which consists mainly of the U1, U2, U4, U5 and U6 snRNP, and about a hundred of non-snRNP proteins. Splicing regulators that bind to enhancer or silencer elements on the pre-mRNA can alter assembly of these spliceosome components. Understanding how splicing regulators control …
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Molecular analysis of peasnRNA variants
Five small nuclear RNAs (snRNAs) (U1, U2, U4, U5, U6) found in the nucleus of eukaryotic cells mediate the excision of introns from pre-mRNAs. The structure and expression of the snRNA components have been well documented in animal and yeast systems but little information has existed on the …
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NMR Studies of the RNA Binding Domain of U1A protein and its Complexes with RNA
… catalysed by five RNA protein complexes called snRNP's. U1A is one protein component of the U1 snRNP particle. The role of U1A in splicing is unclear, but it does bind a hairpin loop from U1 RNA. U1A also binds two internal bulges found in the 3' untranslated region of the mRNA which encodes the …
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Elektronenmikroskopische 3D Strukturbestimmung des Spleißosoms
… der prä-mRNA jeweils neu aus den fünf snRNPs (`small nuclear ribonulceoprotein particles´) und weiteren Faktoren aufgebaut. Der spleißosomale Komplex A, bestehend aus U1 und U2 snRNP, verbindet sich mit dem U4/U6.U5 tri-snRNP, einem Komplex der U4, U5, U6 snRNPs, um den …
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Studies of Atherosclerosis in Systemic Lupus Erythematosus
… regimen of IFNalpha, TLR7 agonists, and U1-snRNP. After 23 weeks, all the monkeys developed positive anti-RNP antibody titers and one monkey developed moderate multifocal degenerative joint disease. For aim two, we treated Sle/LDLr-/- mice with 5.6 µg/day of 17beta -estradiol or placebo …
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Investigating Mechanisms of Nuclear mRNA Export
… such transcripts. Similarly, we demonstrate that U1 snRNP, a component of the spliceosome that recognizes and binds to the 5’SS motif, mediates the nuclear retention of these aberrant mRNAs. We show that ZFC3H1 and U1-70K are not required for targeting 5’SS motif containing mRNAs to nuclear …
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Structural studies of spliceosome assembly and catalysis
… its component small nuclear ribonucleoproteins (snRNPs) and protein factors. A series of conformational and compositional changes produces the catalytically active spliceosome, in which the snRNA components of the snRNPs mediate intron recognition and catalysis of splicing. Despite the importance …