Global ETD Search
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Showing 1 to 5 of 5 for “"Tyrosine recombinase family"”.
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Characterization of IntDOT, the CTnDOT Integrase
… CTnDOT (IntDOT) might be a member of the tyrosine recombinase family because IntDOT has five of six highly conserved residues found in the catalytic domains of tyrosine recombinases. Yet, IntDOT catalyzes a reaction that appears to differ in some respects from well-studied tyrosine …
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Role of the core in CTnDOT integration
IntDOT is the tyrosine recombinase encoded by the Bacteroides conjugative transposon CTnDOT. It catalyzes integration and excision into and out of the bacterial host chromosome. Although it is a member of the well studied tyrosine recombinase family, IntDOT is of particular interest because it can …
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Structure-function analysis of IntDOT, the CTnDOT integrase
… spp. IntDOT has been classified in the tyrosine recombinase family. However, unlike most other tyrosine recombinases that require sequence identity in the region where strand exchanges occur, IntDOT recombines overlap sequences that contain mismatched base pairs. Therefore, IntDOT uses …
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Protein/DNA interactions during site-specific recombination
… which has been identified as a member of the tyrosine recombinase family. Previous DNase I footprinting experiments demonstrated that IntDOT interacts with five arm-type sites, but exactly how the arm-type sites participate in the integration and excision of CTnDOT was not known. I constructed …
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The role of Bacteroides Host Factor A in CTnDOT integration and Holliday junction resolution
… an integrase, IntDOT. IntDOT is a member of the tyrosine recombinase family. It was previously known that a host factor was required for integration and that Escherichia coli Integration Host Factor (IHF) could substitute for the host factor in an in vitro integration assay. I purified DNA …