Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Tyrosine recombinase"”.
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Role of the core in CTnDOT integration
IntDOT is the tyrosine recombinase encoded by the Bacteroides conjugative transposon CTnDOT. It catalyzes integration and excision into and out of the bacterial host chromosome. Although it is a member of the well studied tyrosine recombinase family, IntDOT is of particular interest because it can …
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Coordination and Catalysis of the Lambda Integrase Site -Specific Recombination Reaction
… this work, we concluded that para-Nitrophenol tyrosine analogs are optimal for quantitative in vitro measurement of Int ligation. Int could not utilize tyrosine analogs para-Cresol and dimethyl- p-phenylenediamine as ligation substrates under my reaction conditions. However, they may be useful …
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Characterization of IntDOT, the CTnDOT Integrase
… CTnDOT (IntDOT) might be a member of the tyrosine recombinase family because IntDOT has five of six highly conserved residues found in the catalytic domains of tyrosine recombinases. Yet, IntDOT catalyzes a reaction that appears to differ in some respects from well-studied tyrosine …
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Characterization of the Integrase of the Bacteroides Mobilizable Transposon NBU1
… end (RKHRHY) that are characteristic of tyrosine recombinases. I used site-directed mutagenesis to construct alanine mutants of these six residues. I tested the mutant proteins using an E. coli conjugation assay and showed that the mutants were defective for in vivo integration. This …
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Structure-function analysis of IntDOT, the CTnDOT integrase
… spp. IntDOT has been classified in the tyrosine recombinase family. However, unlike most other tyrosine recombinases that require sequence identity in the region where strand exchanges occur, IntDOT recombines overlap sequences that contain mismatched base pairs. Therefore, IntDOT uses …
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Protein/DNA interactions during site-specific recombination
… which has been identified as a member of the tyrosine recombinase family. Previous DNase I footprinting experiments demonstrated that IntDOT interacts with five arm-type sites, but exactly how the arm-type sites participate in the integration and excision of CTnDOT was not known. I constructed …
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The role of Bacteroides Host Factor A in CTnDOT integration and Holliday junction resolution
… an integrase, IntDOT. IntDOT is a member of the tyrosine recombinase family. It was previously known that a host factor was required for integration and that Escherichia coli Integration Host Factor (IHF) could substitute for the host factor in an in vitro integration assay. I purified DNA …