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Showing 1 to 14 of 14 for “"Type IV Pilus"”.
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Understanding PilB, The Type IV Pilus (T4P) Assembly ATPase
The type IV pilus (T4P) is a dynamic long thin fiber found on the surface of many bacterial groups. T4P is a versatile nanomachine; it plays many important roles such as for surface attachment, virulence factor, and surface motility apparatus. This research focuses on understanding the kinetics of …
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Recruitment and Mechanism of the Bacterial Type IV Pilus Motors
The type IVa pilus system can be used for adhesion, natural competence, phage adsorption, folded-protein secretion, surface sensing, and surface associated motility. Likewise, it is a major virulence factor in many bacterial pathogens. This system extends and retracts a thin and long protein fibre …
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Molecular Analysis of Type IV Pilus Assembly in Clostridium perfringens
Clostridium perfringens is a Gram-positive anaerobe capable of causing disease in humans and many animals. C. perfringens is able to move across surfaces in a manner that is dependent on growth and type IV pili. Type IV pili are filaments that can be extended away from the cell by rapid …
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Characterization of Type IV Pilus System Genes and Their Regulation in Clostridium perfringens
Clostridium perfringens is a Gram-positive (Gr+) anaerobic pathogen that was found to contain Type IV pilus (T4P) system genes within the genomes of all its sequenced strains. T4P are widely used in Gram-negative organisms for aggregation, biofilm formation, adherence, and DNA uptake. Because few …
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Shear force impacts type IV pilus and flagellar dynamics during Pseudomonas aeruginosa surface adhesion
… I examine how Pseudomonas aeruginosa cells use type IV pili to interact with surfaces under host-relevant shear forces. I demonstrate that cell tilting, driven by type IV pilus retraction, predicts surface departure at low shear forces. Conversely, higher host-relevant shear forces counteract …
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Computationally and Experimentally Exploring the Type IV Pilus Assembly ATPase for Antivirulence Drug Discovery
… issue as humanity's existing collection of effective antibiotics dwindles. ABR bacteria are responsible for approximately 5 million deaths worldwide annually, which is predicted to reach 10 million yearly by 2050. Antivirulence therapeutics have been explored in recent times as another approach to …
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Pseudomonas aeruginosa Type IV pilus and resistance to the antimicrobial properties of surfactant protein-A
… Pseudomonas aeruginosa (PA) is a major Gram-negative bacterial pathogen commonly associated with chronic lung infection in cystic fibrosis. In order to resist the antibacterial effects of SP-A, PA expresses various structural and secreted virulence factors. Previously, Wu et al (2003) have shown …
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The Type IV Pilus Assembly ATPase PilB as a Regulator of Biofilm Formation and an Antivirulence Target
Bacterial type IV pili (T4P) are filamentous surface appendages with a variety of functions including motility, surface attachment, and biofilm formation. In many species of bacteria a clear understanding of how the functions of T4P in lifestyle switching are regulated remains to be elucidated. …
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Structure-Activity Relationship Studies of Mitochondrial Uncouplers and PilB Inhibitors
… noncommunicable disease caused by the excessive accumulation of body fat. Rates of diagnosis have been increasing in the late 20th and early 21st centuries, with associated comorbidities also on the rise. Consequently, obesity has become a growing health epidemic globally. Development of …
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Acquisition and Exclusion of Pathogenicity Island 1 (PAPI-1) in Pseudomonas Aeruginosa
… in bacterial evolution and adaptation. Conjugative transfer of genomic islands (GIs) has recently been reported in the opportunistic pathogen Pseudomonas aeruginosa. PAPI-1, one of the largest pathogenicity islands of P. aeruginosa, encodes several putative virulence genes and a major regulator …
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Characterization of an Interaction Between the Pseudopilin XcpT and the Putative ATPase XcpR in the Type II Secretion System of Pseudomonas Aeruginosa
… of these exoproteins is achieved via the Type II secretion system. At least twelve genes named xcp have been demonstrated to encode the gene products responsible for this secretion. The roles for each individual Xcp protein or the protein-protein interactions that would comprise such an …
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Archaeal signal peptides and cell surface structures
… process are the various signal peptidases. Two types of archaeal signal peptidases are identified thus far: signal peptidase I (SPI) and the preflagellin peptidase. SPI is responsible for processing the majority of secreted proteins. Sequence analysis of archaeal SPI enzymes indicates a Sec-11 …
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Delineation of Aeromonas hydrophila Pathotypes by Dectection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay
<p><em>Aeromonas hydrophila </em>is a Gram-negative, bacterial pathogen of humans and other vertebrates. To delineate pathotypes, the genomes of twenty-eight <em>Aeromonas </em>isolates<em> </em>were screened by PCR to determine the presence of virulence factors including: aerolysin …
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Molecular dissection of competence regulated virulence in Streptococcus pneumoniae
… independent of DNA uptake. Competence is activated when the competence stimulating peptide (CSP), encoded by the comC gene, is processed and exported through the ComAB transporter and stimulates the membrane localized two component system sensor kinase, ComD. Activated ComD autophosphorylates …