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Showing 1 to 7 of 7 for “"Type IIS"”.
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REPSA Directed Assessment of Native Cleavage Resistance of DNA to Type IIS Restriction Endonucleases and Modification of REPSA for High Temperature Application
<p>We have modified the combinatorial selection method Restriction Endonuclease Protection and Selection Assay (REPSA) to work in high temperature conditions for the discovery of new DNA-binding proteins in thermophiles (HT-REPSA). We utilized <em>Thermus thermophilus </em>(HB-8/ATCC 27634/DSM 579) …
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The outer spiral network and its innervation by the olivocochlear system
… peripheral projections of olivocochlear (OC) and type-II spiral ganglion neurons. Type-II neurons innervate up to 100 OHCs, but their function is unknown. It has been suggested that type-II neurons interconnect neighboring OHCs through reciprocal synapses, which are bi-directional (afferent and …
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Frameworks for reprogramming early diverging land plants
… and standardised DNA assembly system based on Type IIS restriction enzymes for recursive fabrication of DNA circuits with high efficiency. MarpoDB was used to mine new DNA parts compatible with Loop assembly which were used to generate plant transformation vectors for labelling of cellular …
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Deoxyribonucleic acid restriction/modification systems and gene transfer strategies in Ruminococcus albus 8 and Ruminococcus flavefaciens FD-1
… resulted in the uptake of macromolecules. Type-II endonuclease activities were present in most strains of Ruminococcus tested. However, the cell-free extract of R. flavefaciens FD-1 did not provide a simple DNA protection strategy, so the restriction/modification systems of R. albus 8 and …
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Applications of yeast display in synthetic biology with a focus on biological synthesis and recycling of plastics
… modular cloning toolkit (MoClo-YTK) utilizes type IIS restriction enzymes for the hierarchical assembly of expression vectors from standardised parts into Saccharomyces cerevisiae expression cassettes. In Chapter 3, to facilitate the use of this toolkit for yeast surface display experiments, …
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Analysis of FokI Cleavage Resistance Observed in DNA Sequences Generated via the Combinatorial Selection Method, REPSA
<p>FokI is a thoroughly investigated and highly utilized restriction endonuclease that recognizes the DNA sequence, 5’-GGATG-3’, and cleaves outside of this site 9 and 13 base-pairs downstream. The shifted cleavage function possessed by this kind of endonuclease is utilized in many applications …
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Determination and Dissection of DNA-binding Specificity for the Thermus thermophilus HB8 Transcriptional Regulator TTHB099
… profile data in TTHB099-deficient and wild type strains suggested that, unlike <em>E. coli</em> CRP (CRP<sub>Ec</sub>), TTHB099 does not have a simple regulatory mechanism. However, we hypothesize that TTHB099 can be a dual-regulator similar to CRP<sub>Ec</sub>.</p>