Global ETD Search
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Showing 1 to 14 of 14 for “"Type III Secretion Systems"”.
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Structural and Functional Characterization of SPOA Domains in Salmonella Typhimurium Type III Secretion Systems
… approach anti-infective therapeutic design. Type III secretion systems (T3SS) are employed by some gram-negative human pathogens to manipulate the host environment. One T3SS subtype, known as the "injectisome," delivers virulence factors directly into host cells. The other T3SS subtype …
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Delivering Signal-Altering Bacterial Effector Proteins to Mammalian Cells Using Cell-Penetrating Peptide Technology
… have evolved needle-like structures called Type III Secretion Systems (T3SS) that secrete signal-altering molecules into the host cell to interrupt signaling pathways that would otherwise lead to the elimination of the bacterial infection. These signal-altering molecules are known as …
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A Bioinformatics Analysis of Bacterial Type-III Secretion System Genes and Proteins
Type-III secretion systems (T3SSs) are responsible for the biosynthesis of flagella, and the interaction of many animal and plant pathogens with eukaryotic cells. T3SSs consist of multiple proteins which assemble to form an apparatus capable of exporting proteins through both membranes of …
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Characterization of VIBRIO Parahaemolyticus-Induced Intestinal Inflammation in the Mouse
… of the pathogen. V. parahaemolyticus utilizes a type III secretion system to inject effectors that disrupt signal transduction in eukaryotic hosts during infection. Herein, we describe an in vivo model of infection by V. parahaemolyticus using germfree mice. V. parahaemolyticus is recovered from …
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Characterization of Vibrio VopS, an AMPylator of Rho GTPases
… a clinical isolate revealed the presence of two type III secretion systems (T3SSs), one on each chromosome. The T3SS on chromosome one (T3SS1) has been shown to be responsible for cytotoxicity in HeLa cells, and it shares a high degree of homology to the T3SS of the Yersinia spp. Our studies have …
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Analysis of Vibrio parahaemolyticus Virulence Systems
… factors, including two hemolysins and two type III secretion systems (T3SS1 and T3SS2). Herein, we compare the virulence of V. parahaemolyticus POR strains, which harbor a mutation in the T3SS needle apparatus, to the V. parahaemolyticus CAB strains, which contain mutations in …
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Structural and functional studies of type three secretion virulence factors from gram-negative pathogenic bacteria
Many pathogenic Gram-negative bacteria utilize type III secretion systems (TTSS) to alter the normal functions of target host epithelial cells. Of the 1.1 million deaths that are caused by Shigella each year, nearly a third are in children under five years of age. Salmonella enterica serovar …
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Analysis of Small Molecule Inhibitors Aimed at Bacterial Virulence
… Genetic and biochemical studies have highlighted type III secretion systems (T3SSs) as essential components for infection of host cells by Gram-negative bacterial pathogens. Small molecules that target type III protein secretion may therefore represent a new class of antibacterial agents and …
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Investigation of Pantoea stewartii Quorum-Sensing Controlled Regulators and Genes Important for Infection of Corn
… sequence permitted genes for two distinctive Type III secretion systems, used for interactions with corn or the corn flea beetle, to be placed on two mega-plasmids. Furthermore, the complete genome sequence facilitated a Tn-Seq approach (Chapter Five). Tn-Seq is a potent tool used to identify …
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Characterization of Mitochondrially Targeted T3SS2 Effectors in Vibrio parahaemolyticus
… All strains of V. parahaemolyticus harbor the type III secretion system 1 (T3SS1), which is dispensable for virulence in humans. Pathogenic strains harbor an additional type III secretion system (T3SS2) that mediates host cell invasion to form a protective replicative niche and is responsible …
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Characterisation of the structure and function of the Salmonella flagellar export gate protein, FlhB.
… and this is achieved by a specialised flagellar Type III Secretion System (fT3SS) located at the base of each flagellum. The fT3SS imposes a crude ordering of subunits, with filament subunits only exported once the rod and hook are complete. This “export specificity switch” is controlled by the …
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Tale of Two Hosts: Vibrio parahaemolyticus Toxicity Against Human and Shrimp
… VtrB, are required for activating the virulence type III secretion system 2 (T3SS2) in response to bile salts. The VtrA/VtrC complex activates VtrB in the presence of bile salts. The crystal structure of the periplasmic domains of the VtrA/VtrC heterodimer reveals a β-barrel with a hydrophobic …
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Tale of Two Hosts: Vibrio parahaemolyticus Toxicity Against Human and Shrimp
… VtrB, are required for activating the virulence type III secretion system 2 (T3SS2) in response to bile salts. The VtrA/VtrC complex activates VtrB in the presence of bile salts. The crystal structure of the periplasmic domains of the VtrA/VtrC heterodimer reveals a β-barrel with a hydrophobic …
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Evidence for Organelle-Like Extracellular Vesicles from a Parasite of Drosophila and Their Function in Suppressing Host Immunity
… wasps act as keystone species in natural ecosystems. Adept at suppressing immunity of their insect hosts, these natural enemies of insect pests are used for biocontrol in many parts of the world. Female parasitic wasps of the closely-related species <em>Leptopilina heterotoma</em> …