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Showing 1 to 9 of 9 for “"Type II secretion"”.
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Structural characterization of the type II secretion system of Aeromonas hydrophila
The exeC gene, found in the gram-negative bacteria Aeromonas hydrophila codes for a 31 kDa, three domain, bitopic inner membrane protein. The components of the ExeC protein include an amino-terminal cytoplasmic domain, a trans-membrane helix and two periplasmic domains. The two periplasmic domains …
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Characterization of an Interaction Between the Pseudopilin XcpT and the Putative ATPase XcpR in the Type II Secretion System of Pseudomonas Aeruginosa
The secretion of alkaline phosphatase, phospholipase C, lipase, elastase, and exotoxin A are among several virulence factors that contribute to the opportunistic pathogenicity of Pseudomonas aeruginosa. The secretion of these exoproteins is achieved via the Type II secretion system. At least twelve …
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Type IV Pili-Dependent Secretion of Biofilm Matrix Material Proteins in Clostridium perfringens
… to identify pilin mutants that are needed for secretion of the biofilm matrix and develop a functional model for a type II secretion system (T2SS) in C. perfringens. Protein tagging, western blot, and slot blot experiments were done to quantify protein secretion. After performing experiments …
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A Mutational Analysis of XcpQ, the Outer Membrane Component of the Type II Protein Secretion System From Pseudomonas Aeruginosa
… and LipC cryptic lipase are exported via a Type II secretion apparatus. XcpQ is the sole outer membrane protein component of this system and is a member of the superfamily of proteins called secretins. XcpQ forms extremely stable pore-like outer membrane multimers, through which Type II …
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Exoproteomics of the Pseudomonas aeruginosa biofilm matrix reveals the role of a novel secretion system
… of cells living in a matrix of their own secretions. Cells that would otherwise be susceptible to antimicrobials are protected by the modifications that they make, in concert, to their surroundings. Aggregates of as few as 64 cells begin to exhibit elevated tolerance to antibiotics and, …
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Use of an Inducible Promoter to Characterize Type IV Pili Homologues in Clostridium perfringens
… deletion mutants in three genes with homology to Type IV pilins, and we used the promoter system described above to complement the mutants. We analyzed each pilin for localization and expression, as well as tested each of the mutants for various phenotypes frequently associated with type IV pili …
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A Simulation Approach to the Study of Bacterial Secretion Proteins
… the complex machinery associated with the type II secretion system (T2SS) polymerises inner membrane pseudopilin proteins into thin filaments, to export substrates such as toxins, hydrolases and cytochromes. Here, computational simulations were used to study proteins from the Klebsiella …
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Studies on the architecture and on transferability of pathogenicity islands of uropathogenic Escherichia coli strain 536
… of the capsule determinant, the genes encoding a type II secretion system (general secretion pathway -GSP) are located on PAI V536. The K15 capsule locus was functionally characterized. Specific inactivation of each of the regions 1 to 3 of the kpsK15 gene cluster, and the use of a K15 …
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The role of abscisic acid in the defence response of tomato (Solanum lycopersicum) to the necrotrophic pathogens Botrytis cinerea and Erwinia chrysanthemi
… of gene expression in sitiens and wild-type tomato with TOM1 microarrays revealed that defence-related transcript accumulation prior to infection is higher in sitiens than in wild type. Moreover, further elevation of defence gene expression after pathogen attack is also stronger in …