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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Type II Collagen"”.
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Type II collagen degradation during experimental (osteo)arthritis
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Cell-seeded type II collagen scaffolds for articular cartilage tissue engineering
… environment on chondrogenesis in cell-seeded type II collagen scaffolds in vitro, and to begin to test effects of implant compositional maturity (viz. glycosaminoglycan, GAG, content) on chondral defect repair. Scaffold cross-link density, a determinant of cell-mediated scaffold contraction …
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The Extraction of Type II Collagen and the Electrospinning of Nano-Fibrous Scaffolds
… functions to reduce friction and absorb shock. Collagen type II is the largest constituent in the extracellular matrix of articular cartilage and its restoration is of the highest interest to tissue engineers. Cartilage has little ability to naturally regenerate due to the absence of vascularity …
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Design of an Electrospun Type II Collagen Scaffold for Articular Cartilage Tissue Engineering
… restoring functional tissue. In this research, type II collagen was electrospun, cross-linked, and tested as scaffolds for supporting chondrocyte growth. The mechanical, biochemical, and histological characteristics of the engineered tissue were assessed as a function of the electrospinning …
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The effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type II collagen scaffolds
… to cause the goat articular chondrocyte seeded type II collagen scaffolds to generate 25 and 50% of the natural biochemical content of articular cartilage, with an overall goal of identifying construct compositions that might provide the most favorable response when implanted into defects in …
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Method for quantitative analysis of glycosaminoglycans and type II collagen in chondrocyte-seeded articular cartilage scaffolds with varied cross-linking density
… cartilage are produced by cross-linking type II collagen with glycosaminoglycans (GAG), creating pores in the resulting construct, and then seeding these pores with chondrocytes (articular cartilage producing cells). However, little information is known regarding the effect of …
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An Analysis of the Collagen Synthesized by Chondrocyte Cultures Derived From the Chick Embryo Tibiotarsus
… tibiotarsus synthesize chondroitin sulfate and type II collagen in primary and secondary cultures. The collagen is secreted into two extracellular collagen pools, the medium (CM) and matrix (Ma) fractions. The CM-collagen fraction contains procollagen and the intermediates in its metabolic …
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CONNECTIVE TISSUE GROWTH FACTOR IS REQUIRED FOR CHONDROGENESIS
… early chondrogenic markers (sox9, sox6, l-sox5, type II collagen, aggrecan, decorin and link protein). Real-Time PCR, Western blot and Immunofluorescence reveal that CCN2 is required for the early stages of chondrogenesis, including expression of sox6 and aggrecan, but not type II collagen. …
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Application of Cell-Derived Extracellular Matrix (ECM) Scaffold on Tissue Engineering Cartilage
… time. The DNA, glycosaminoglycan (GAG) and collagen contents also increased gradually with time. Histological staining for GAG (Safranin O staining) and type II collagen (immunohistochemistry) showed sustained accumulation of the ECM molecules along with time, which gradually and uniformly …
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Functional Tissue Engineering of Cartilage Using Adipose-derived Stem Cells
… of dynamic loading induced the production of type II collagen, a principal component of articular cartilage.</p><p>We hypothesized that a biomaterial derived from cartilage would serve as a bioactive scaffold and induce chondrogenic differentiation. The novel, ECM-derived scaffold promoted the …
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Gene-supplemented collagen-glycosaminoglycan scaffolds for nonviral gene delivery in articular cartilage tissue engineering
… of this thesis was to develop a method to employ type II collagen-glycosaminoglycan (CG) scaffolds for the nonviral delivery of the gene encoding for insulin-like growth factor (IGF)-I, as a novel means to provide a local, elevated, and prolonged release of a therapeutic growth factor via …
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Probing nanomechanics of aggrecan and the aggrecan-rich pericellular matrix of chondrocytes in cartilage
… with the extracellular matrix network of type II collagen and the proteoglycan, aggrecan, which in combination provide the tensile, shear, and compressive stiffness of the tissue. While the collagen network mainly provides resistance to tensile and shear deformation, aggrecan enmeshed …
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Nanomechanics of cartilage extracellular matrix macromolecules
… mechanical properties of cartilage and its type II collagen network was measured. Aggrecan shear nanomechanics was assessed via microcontact printing and lateral force microscopy. Lateral force between aggrecan and the probe tip, and compression of aggrecan was simultaneously measured in …
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In vitro culture of a chondrocyte-seeded peptide hydrogel and the effects of dynamic compression
… a cartilage-like ECM rich in proteoglycans and type II collagen, indicative of a stable chondrocyte phenotype. Time dependent accumulation of this ECM was paralleled by increases in material stiffness, indicative of deposition of mechanically-functional neo-tissue. Culture of chondrocyte-seeded …
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Effects of biochemical and mechanical stimulation of articular chondrocytes in collagen-GAG scaffolds : extracellular matrix biosynthesis and scaffold stiffness
… Numerous reports indicate that certain types of mechanical loading can increase chondrocytes' ECM biosynthesis in particular cell-scaffold systems in vitro. Few if any loading experiments have been done, however, with type II collagen-GAG scaffolds cultured in serum-free medium. Chapter …
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Modulation of Alzheimer’s Disease Pathology by Collagen-Induced Rheumatoid Arthritis in APP/PS1 Mice
… was performed to directly explore the effects of collagen-induced rheumatoid arthritis (CIA) on cognitive function, amyloid plaque formation, microglial activation, and cerebrovascular pathology in the cortex and hippocampus of the double transgenic APP/PS1 mouse model for AD. Wild-type or APP/PS1 …
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The role of DEL1 in chondrocyte expansion and mechanotransduction: an investigative study
… challenged by loss of differentiated phenotype upon culture. To investigate the mechanism of dedifferentiation, chondrocytes were cultured at low density (1x104cell/cm2) and subcultured on days 9 (P1), 14 (P2) and 21 (P3). A loss of a differentiated phenotype was observed in P1 with a …
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Characterization and tissue engineering of the temporomandibular joint disc
… cell sub-population distribution and anisotropic collagen orientation. The greatest variation by region in the TMJ disc is between the intermediate zone and the bands (anterior and posterior). The intermediate zone contains a higher proportion of fibrochondrocytes and higher quantities of type II …
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Identifying sm22 as a key player in arterial diseases
… (Sm22<sup>-/-</sup>) mice and their wild type littermates were subjected to carotid artery denudation, an artery injury model. Analyses were conducted on carotid arteries 2 weeks after injury. Primary VSMCs were isolated from mouse aortas and investigated individually at passage 2 to 4. …
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The human meniscus: transplantation, characterisation and tissue engineering
… with a viable allograft. The results of this type of surgery are encouraging and long-lasting in a well-selected patient population who suffered a total meniscectomy. Viable meniscus allograft transplantation significantly reduces pain and improves function. This beneficial effect remains …
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