Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 91 for “"Type I Collagen"”.
-
Type I collagen proteostasis
The folding, quality control, and secretion of collagen presents a significant challenge to collagen-producing cells. Each monomeric polypeptide must undergo extensive post-translational modifications, folding and assembly of the C-terminal propeptide globular domain, and isomerization of hundreds …
-
Mechanical behavior of individual type I collagen fibrils
… of studies on the mechanical response of collagen, especially at the molecular scale or at much larger length scales, such as of those of fibers, tendons, and fascicles, there is still limited information about the mechanics of collagen fibrils (50 - 500 nm diameter) that serve as …
-
The regulation of type I collagen gene expression in stromal fibroblast by breast tumour cells
… (ECM) components found in the stroma, including type I collagen. Previous in vivo studies in our laboratory have shown that type I collagen mRNA levels are decreased in stage II and III breast tumour tissue compared to adjacent normal tissue.
-
The role of type I collagen heterotrimers and homotrimers in mechanical strength and collagen cleavage
Collagen is a crucial structural protein, formed through a hierarchical assembly of molecules, arranged in collagen fibrils, which constitutes the basis for larger-scale fibers. Normal type I collagen is a heterotrimer triple-helical molecule consisting of two alpha-1 chains and one alpha-2 chain. …
-
Effects of type-I collagen fractional composition and pyridinium crosslink content on cortical bone strength in the human femur
Type-I collagen's role in the formation of bone and its inherent strength have been well documented by studies in genetic diseases such as Osteogenesis Imperfecta. Type-I collagen's role in healthy bone, and the changes that occur to collagen during aging, which may eventually lead to osteoporosis, …
-
Glomerulosclerosis in the Col1a2-deficient mouse model : homotrimer pathogenesis and MMP expression
… mouse model exclusively synthesizes homotrimeric type I collagen due to the lack of functional pro [alpha] 2(I) collagen chains. The mouse develops a type I collagen glomerulopathy that has previously been shown to initiate postnatally and progress in a gene dose-dependent manner, accumulating …
-
Matrix-mediated regulation of type 1 collagen synthesis and degradation in cultured fibroblasts
… of most of the extracellular matrix proteins. Type I collagen is the most abundant extracellular matrix protein in the human body and is found in tissues requiring high tensile strength. In this study we investigated the effect of a pre-formed fibroblast-derived extracellular matrix on the …
-
The extraction of type 1 collagen and the fabrication of multi-filament embedded hydrogels for guided nerve regeneration
… experiments by using natural materials such as type I collagen, which is the largest constituent of the extra- cellular matrix of living tissue. In this project, fabrication of novel constructs for nerve tissue guidance was carried out using homogenous hydrogels and multi-filament arrays of …
-
Investigation of Keratin and Keratin-Containing Composite Biomaterials: Applications in Peripheral Nerve Regeneration
… blended material comprised of said keratose and type I collagen was undertaken. It was hypothesized that a blend would result in mixing at the molecular level, resulting in improved properties compared to either pure material alone. A protocol was created to make stable keratose/type I collagen …
-
Collagen gene expression in human cancer
Type I collagen is the predominant collagen within the stroma and plays an important role in the processes of tumour cell invasion and metastasis during which the collagens within the stroma is degraded. Total RNA was extracted from different stages of breast cancer and adjacent normal tissue for …
-
NOVEL NANOFIBER-BASED GRAFT FOR HEART VALVE REPLACEMENT
… tissue engineering. While processed conventional collagen-based heart valves have been used for many years, they eventually fail because of inadequate cell infiltration and insufficient replacement of graft material by tissue remodeling. To overcome the graft failure due to inadequate tissue …
-
The role of DNA methylation in transcriptional regulation of the human type 1 alpha 2 collagen (COL1A2) gene
Type I collagen is the most abundant collagen molecule in vertebrate connective tissue and it consists of a heterotrimer of two alpha 1 (COL1A1) and one alpha 2 (COL1A2) chains. Reduced collagen gene expression is almost always correlated with pathological conditions and cellular transformation. …
-
A tissue-engineered approach to hernia repair
… (LEVO) (a broad-spectrum antibiotic). Type I collagen was later included into the polymer-drug solutions in order to increase the biocompatibility of the samples. Electrospun samples were subsequently analysed for mechanical, physicochemical, drug release and biological characteristics. …
-
Fabrication and evaluation of a collagen-based fiber-gel three-dimensional construct for peripheral nerve repair
… nervous tissue constructs consisting of type I collagen are fabricated into a composite biomaterial scaffold to promote contact-guided growth of neuronal and glial cultures in vitro. The growth of adult tissue on these collagen-based materials is further evaluated. These constructs are …
-
The Treatment of Breast Cancer Tumor Growth and Metastasis With an Anti-MMP9 DNAzyme
… and chemokines. MMP-9 is known to cleave type IV collagen, one of the most abundant collagens of the extracellular matrix. Our lab has found that the fully processed (68 kDa) form of MMP-9 generated in tumor and stromal cells is also capable of cleaving type I collagen molecule with …
-
Analisi morfologica e molecolare di cellule da colture primarie umane esposte a resine biocompatibili
… exposition on specific proteins such as type I collagen and tenascin has not been studied in depth. The objective of this project was to study the effect of minor toxic concentrations of HEMA on human gingival fibroblasts (HGFs) and human pulp fibroblasts (HPFs), investigating …
-
FUNCTIONALIZATION OF A NOVEL ELASTOMERIC BIOMATERIAL FOR VASCULAR TISSUE ENGINEERING
… poly(glycerol sebacate), silk fibroin, and type I collagen (PFC) has properties ideal for use as a tissue engineered scaffold for an artery graft. The long-term goal of this project was to develop a functionalized PFC scaffold for use as a small diameter vascular graft that would facilitate …
-
DRUG ELUTING TISSUE ENGINEERED VASCULAR GRAFTS
… a macrofiber diameter poly(ε-caprolactone) (PCL) type I collagen outer layer that promotes vascular smooth muscle integration and media layer formation, a nanofiber diameter PCL type I collagen middle layer that promotes endothelial monolayer formation, and a microfiber poly(lactic-co-glycolic …
-
The potential role of innate immunity in the pathogenesis of post-operative adhesions
… and differentiation of several cell types including mesothelial cells, fibroblasts, and inflammatory and immune cells. Substances released from these cells regulate fibrinolytic activity, tissue remodeling, angiogenesis, and extracellular matrix turnover, processes that are known to …
-
The human meniscus: transplantation, characterisation and tissue engineering
… with a viable allograft. The results of this type of surgery are encouraging and long-lasting in a well-selected patient population who suffered a total meniscectomy. Viable meniscus allograft transplantation significantly reduces pain and improves function. This beneficial effect remains …
Page 1 of 5