Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 29 for “"Two-component systems"”.
-
Characterization of two component systems of Acinetobacter baumannii.
… A. baumannii, in particular those mediated by two component regulatory systems (TCSs), that typically consist of a membrane bound sensor kinase and a cognate response regulator. Bacterial TCSs play an important role in the regulation of adaptation to different environmental conditions. Five …
-
Threonine Phosphorylation Regulates Two-Component Systems Involved in Cell Wall Metabolism
… this has been studied in the context of two-component systems (TCS). TCS are composed of a sensor histidine kinase that upon activation autophosphorylates a conserved histidine residue and then transfers this phosphate to a response regulator at a conserved aspartate residue. This …
-
High-Throughput Discovery of Stimuli of Bacterial Two-Component Systems from the Human Gut Microbiome
… and have major impacts on health and disease. Two-component systems (TCS) are the primary means by which bacteria, including those in the gut microbiome, sense and adapt to their environment. The prototypical TCS consists of a membrane-bound sensor histidine kinase (SHK) and a cytoplasmic …
-
Photosensing with LOV: Molecular Insights into the Shared Mechanism of Light-Regulated Kinase Signaling between Plant Phototropins and Bacterial Two-Component Systems
With the overall goal of understanding the basis of Light–Oxygen–Voltage (LOV) domain mediated signaling transduction pathways, my graduate research focused on characterizing the role of LOV domains in kinase signaling and the mechanism of signal propagation from LOV domains to a diverse set of …
-
PhoQ: Structural and Mechanistic Investigations into an Important Bacterial Sensor Kinase
Two-component systems represent the dominant mechanism for cellular signal transduction in prokaryotes. One particular system, PhoQ, has been the focus of our interest because it is the switch that controls virulence in Salmonella and other pathogenic gram negative bacteria. Certain domains of PhoQ …
-
On some classes of birth and death processes in continuum
… in random environment are treated. Additionally, two-component systems are introduced: the ecological model and Glauber-Potts type model. For all of the above-mentioned processes, the existence of the associated dynamics is proven. The Vlasov-type scaling is applied to the two-component models and …
-
Evolution-Inspired Design of Bacterial Biosensors
Bacterial signal transduction systems are critical tools for synthetic biology. However, while hundreds of thousands of systems have been identified in bacterial genomes, only a small subset have been characterized. In this work, we develop tools for scalable interrogation of bacterial signal …
-
Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in V. alginolyticus
… of and orientation of the domains hint that the two target different regions in collagen. Study small angle x-ray scattering (SAXS) was used to give a low-resolution envelope of full-length Col G and Col H and Col G/Col H non-catalytic domains bound to a collagen-like peptide (mini-collagen). …
-
Mechanism and specificity of bacterial two-component signaling systems
Bacterial two component signaling (TCS) systems are the predominant means by which bacteria sense and respond to external signals. These systems represent a large family of paralogous proteins; often hundreds of the histidine kinase (HK) and response regulator (RR) pairs that make up a TCS system …
-
Gene Regulation by a Novel Two-Component System Conserved Among Gammaproteobacteria
<p>Two-component systems are common gene regulatory pathways seen in bacteria. Gene expression is controlled by these systems through a series of phosphorylation events between two proteins, a membrane bound sensor kinase and a cytoplasmic response regulator. Activation of a two-component system …
-
Structural Studies of the Bacterial Histidine Kinases RetS and GacS, Key Components of the Multikinase Network that Controls the Switch Between a Motile Invasive Lifestyle and a Sessile Biofilm Lifestyle in Pseudomonas aeruginosa
Signal transduction networks enable organisms to respond to environmental stimuli. Bacteria utilize two-component systems (TCSs) and phosphorelays as their primary means of signal transduction. Histidine kinase (HK) and response regulator (RR) proteins comprise these TCSs and phosphorelays. …
-
Alternative regulation of the alginate algD operon by an activated AlgB in nonmucoid Pseudomonas aeruginosa is dependent on Sigma 54
… is AlgB, a member of the NtrC family of two-component systems, which typically utilize σ54. However, neither σ54 nor the cognate sensor kinase (KinB) of AlgB are required for algD expression in such mucoid strains. I hypothesized that KinB-phosphorylated AlgB must play some role in gene …
-
GENE REGULATION CONTROLLED BY THE CRBS/R TWO-COMPONENT SYSTEM
… and respond to environmental changes is through two-component systems (TCSs). TCSs are composed of a membrane bound sensor protein that can pick up an environmental cue that then activates a cytoplasmic response regulator. The response regulator can, in turn, bind DNA and either activate or …
-
Structural and Functional Studies of Sensor Kinase RetS from Pseudomonas aeruginosa and Peptidoglycan Hydrolase SleB from Bacillus anthracis
… gene expression, Pseudomonas aeruginosa utilizes two-component regulatory systems (TCS). Specifically, we focus on the TCS signaling kinase RetS, which is a critical repressor of biofilm formation. The signaling mechanism of RetS is unusual. According to recent findings and one hypothesis, RetS …
-
Impact of environmental conditions on expression levels of resistance-nodulation-division (RND) efflux pumps and outer membrane porins in Acinetobacter baumannii
… difference in expression levels of the selected two-component systems between a type strain of A. baumannii, ATCC 19606, and two clinical isolates with the MDR and biofilm-forming phenotypes. The quantitative-real time polymerase chain reaction analysis revealed that levels of iron(III) in growth …
-
Dusty plasmas with high electron depletion
… density determination are developed. A neural-network-based evaluation of polarized light scattering (HERMiNe) accelerates particle size reconstruction compared to CRAS-Mie fitting while enabling a detailed uncertainty analysis. In addition, the video-aided extinction measurement (VAEM) removes …
-
A methodology to evaluate photovoltaics: storage as a commercial customer initiated demand side management tool
… PV storage system is greater than either of the two component systems taken alone. By adding battery storage to the PV system, the optimum system size was increased.
-
Structural Basis of Bacterial Transmembrane Signaling
… incompletely understood. My research focuses on two distinct bacterial signaling systems: the methyl-accepting chemotaxis protein McpZ in Sinorhizobium meliloti and the signaling histidine kinase GacS in Pseudomonas aeruginosa. What made this work particularly challenging is that the ligands that …
-
Biophysical and Biochemical Characterization of a REC Domain: Unfolded to Folded Transition of EL_LovR
Prokaryotes frequently use two component systems to couple environmental stimuli to adaptive responses. These pathways use histidine kinases to detect environmental cues, harnessing these to control phosphorylation of the receiver domain of the response regulator, which convert this signal into a …
-
The Characterization of the Fucose Sensing Kinase (FUSK) and the Fucose Sensing Response Regulator (FUSR) and Their Role in Virulence Regulation in Enterohemorrhagic Escherichia Coli O157:H7
… AI-3/Epi/NE interkingdom signaling cascade. The two-component systems QseBC and QseEF are at the core of the AI-3/Epi/NE signaling, controlling expression of flagellar motility genes, the LEE and type 3 secreted effectors in response to AI-3 and the catecholamine hormones Epi and NE. The network …
Page 1 of 2