Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 62 for “"Two-Component System"”.
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GENE REGULATION CONTROLLED BY THE CRBS/R TWO-COMPONENT SYSTEM
… and respond to environmental changes is through two-component systems (TCSs). TCSs are composed of a membrane bound sensor protein that can pick up an environmental cue that then activates a cytoplasmic response regulator. The response regulator can, in turn, bind DNA and either activate or …
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Gene Regulation by a Novel Two-Component System Conserved Among Gammaproteobacteria
<p>Two-component systems are common gene regulatory pathways seen in bacteria. Gene expression is controlled by these systems through a series of phosphorylation events between two proteins, a membrane bound sensor kinase and a cytoplasmic response regulator. Activation of a two-component system …
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Induction kinetics of the PhoQ-PhoP two-component system in Escherichia coli
Cells rely on signal transduction systems to sense and respond to changes in their enviroments. When a stimulus is present, the corresponding signal transduction system will activate and enact the appropriate biological response, often by modulating target gene expression. In many cases, the …
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Characterization of the Activation of the FlgSR Two-Component System in Campylobacter Jejuni
… the flagellar export apparatus, sigma54, and a two-component regulatory system comprised of the FlgS sensor kinase and the FlgR response regulator to activate transcription of the middle and late sigma54-dependent flagellar genes. The FlgR response regulator is an NtrC-like protein that can be …
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Signaling Specificity in a Campylobacter jejuni Two-Component System to Mediate Proper Flagellar Gene Transcription
… discovered the flagellar type III secretion system (T3SS), the FlgSR two-component regulatory system (TCS), and the FlhF GTPase as requirements to positively regulate expression of σ54-dependent genes that encode flagellar rod and hook proteins. Our laboratory previously proposed that signal …
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Engineering a cyanobacteriochrome two-component system into a synthetic, light-controlled gene expression system for plants
… we would like an orthogonal, genetically encoded system, that allows us to reversibly perturb the expression of genes of interest with maximal spatiotemporal resolution in a minimally invasive way. Our lab has reengineered the CcaS/R light receptor two-component system from cyanobacterium …
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Studies on the role of CheS in Sinorhizobium meliloti chemotaxis
… attractants and away from repellents. The two-component system controlling chemotaxis in bacteria contains a histidine kinase CheA, which is autophosphorylated in response to a signal from a ligand-bound transmembrane methyl-accepting chemotaxis protein. CheA transfers the phosphate group …
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A novel two-component signal transduction system in propionibacterium acnes and its association with a putative extracellular signalling peptide
… identified a putative unique quorum sensing system of P. acnes, consisting of a putative signalling peptide, and divergently transcribed histidine kinase and response regulator. The aim of this project is to investigate the relationship among these three components as being elements of a …
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Multiple Posttranscriptional Regulatory Features Control Expression of Ethanolamine Utilization Genes In Enterococcus Faecalis
… faecalis</em>. I discovered the presence of two unique posttranscriptional regulatory features that control expression of <em>eut</em> locus genes. The first feature I found is an AdoCBL riboswitch, a <em>cis</em>-acting RNA regulatory element that acts as a positive regulator of gene …
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Specificity and evolution of bacterial two-component signal transduction systems
… After duplication, however, all pathway components are identical in sequence and function. To evolve a new role, the pathways must become insulated at the level of signal transduction. Two-component signal transduction systems, consisting of a sensor histidine kinase and a cognate …
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Polysaccharide utilization by the human colonic bacterium, bacteroides intestinalis DSM 17393
… period, generating an intrinsic metabolic network capable of modulating both the microbial community and host physiology. Changes in the gut environment shape the microbial community with one of the main driving characteristics being competition for nutrients. A key aspect for nutrient …
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The Impact of Selected Gene Mutations to the Activation and DNA Binding Activity of the Transcription Factor VraR
VraSR two-component system in S. aureus senses bacterial cell wall damage caused by antibiotics (vancomycin or -lactams) and regulates a set of genes. We have undertaken the study of the role of certain amino acids in the function of VraR, which undergo mutation in clinical S. aureus resistant …
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Posttranscriptional and posttranslational regulation of virulence in erwinia amylovora
… bacterial disease of the Rosaceae family plants. Two virulence factors, the type III secretion system (T3SS) and the exopolysaccharide (EPS) amylovoran, are strictly required for its pathogenicity. Our previous studies have determined the role of several transcription factors in the regulation of …
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Engineering Materials for Non-Compressible Torso Hemorrhage and Internal Bleeding
… exposed wound, or interact with wound-specific components when delivered intravenously to enhance preexisting hemostatic processes. The first part of this thesis investigates the effect of hemostatic nanoparticle size on their interactions with platelets. Small nanoparticles were observed to …
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Structural investigations of adenosylcobalamin-dependent enzyme maturation
… through GTP hydrolysis. In addition to the human system, there are two bacterial systems used to understand the maturation of MCM: a homologous three-component system from Methylobacterium extorquens and an analogous two-component system from Cupriavidus metallidurans. The two-component system …
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Serine/threonine phosphorylation in mycobacterium tuberculosis : identification of protein kinase B (PknB) substrates
… serine/threonine kinases PknB and PknH with a two-component system response regulator DevR, and further proposed Ser/Thr phosphorylation as the negative regulator of DevR transcription activator activity based on LC-MS/MS analysis. Finally, we have identified a previously unknown phosphoprotein …
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Measuring and Modeling The Response Characteristics Of The Environmental Phosphate Transducer In Escherichia Coli
<p>The PhoR/PhoB two-component system in <em>Escherichia coli</em> is a biological transducer that senses the limitation of environmental inorganic orthophosphate, the bacteria's preferred source of the essential nutrient phosphate, and transmits that information to the interior of the cell …
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Similarities and variations of the enterobacterial chemotaxis paradigm in Sinorhizobium meliloti
… inhabit. This symbiosis serves as a great model system for studying biological nitrogen fixation and plant-microbe interactions. Since alfalfa brings enormous economic value to the USA, investments into the knowledge of the chemotaxis process that initiates symbiosis have the ability to mitigate …
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FLP-mediated conditional loss of an essential gene to facilitate complementation assays
… and time-consuming step, the newly developed two-component system utilizes a site-specific recombinase to excise a wild-type copy of the gene of interest from transformed tissues. In the first component (the first vector), a wild-type version of the gene is placed between target sequences …
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