Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"Two dimensional Polyacrylamide Gel Electrophoresis"”.
-
Detection of Unique Tumor-Associated Proteins in the Urine of Patients with Transitional Cell Carcinoma by High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis
<p>A two-dimensional polyacrylamide gel electrophoretic technique was developed for the detection of unique tumor-associated proteins in urine of patients with transitional cell carcinoma (TCC) of the urinary bladder. This technique yielded high resolution of polypeptides in the urine of cancer …
-
Stress response of porphyromonas gingivalis
… Porphyromonas gingivalis was examined by one-and two-dimensional polyacrylamide gel electrophoresis after metabolic labeling with (14C)-amino acids. When P. gingivalis cells were shifted from 37°C to 42°C, elevated synthesis of four proteins with the apparent molecular weight of 92, 80, 74, and …
-
Implementation of nano-liquid chromatography hyphenated to tandem mass spectrometry for protein identification in gel and non-gel based proteomics
… describes the analysis of cytosolic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. First, the techniques are highlighted that were used to identify gel separated proteins, i.e. the implementation of nano-liquid chromatography and mass spectrometry (Chapter …
-
Response of Leaf Protein to Ozone in Two White Clover Clones
… Superoxide dismutase isozyme activities in the two clones did not respond differently to ozone treatment. However, catalase activity increased somewhat more in the leaf tissue of NC-R, compared to NC-S, after ozone treatment. Two-dimensional polyacrylamide gel electrophoresis (PAGE) showed the …
-
Comparison of induced proteins synthesized in hydrogen peroxide and near-ultraviolet -treated bacterial cells
… to compare the proteins that were induced by the two agents, as well as the proteins that cease synthesis under these conditions, using two-dimensional polyacrylamide gel electrophoresis (PAGE). Also, specific comparisons were made of catalase (HPI and HPII) and superoxide dismutase (Fe2 + and Mn2 …
-
Studies on two nickel-containing enzymes from Methanosarcina thermophila TM-1
… Methanosarcina thermophila was examined by two-dimensional polyacrylamide gel electrophoresis to determine the extent of regulation by the growth substrate. More than 100 mutually-exclusive spots were present in acetate- and methanol-grown cells suggesting a high degree of regulation. Spots …
-
The Mycobacterium smegmatis "Proteome" : effects of growth phase on total protein synthesis and on the response to heat shock
… expression profiles analysed using one- and/two-dimensional polyacrylamide gel electrophoresis, autoradiography, and/or immunoblot analysis. The ATP content of the culture was found to be a more accurate indicator that cells were entering stationary phase than the number of colony forming …
-
The proteomic analysis of B-cell receptor signaling in chronic lymphocytic leukaemia
… die of a chemotherapy resistant disease within two years of presentation. Genomic studies have found little variation in patients showing differing prognosis, suggesting that it is the same disease but with varying outcomes. At present there is no cost effective, reliable and routine clinical …
-
Proteomic analysis of Pseudomonas putida F1 during growth on toluene-phenol mixtures
… or a toluene-phenol mixture were analyzed by two-dimensional polyacrylamide gel electrophoresis. Three groups of proteins (Group T. P. and M). which were differentially produced when the bacterium grew on toluene, phenol, or the toluene-phenol mixture, respectively, were categorized by two …
-
Applications of Mass Spectrometry to Analysis of Prodiginines, Bioactivated Methylenedianiline Intermediates, and Hypoxia Induced Changes in the Zebrafish Skeletal Muscle Proteome
… coupled with liquid chromatography and gel electrophoresis enables separation and detection of components in a complex mixture. During the last two decades, its applications were dramatically extended and remarkable progress has been made in many fields, in particular, environmental and …
-
Patterns of protein expression in tissues of the killifish, Fundulus heteroclitus and Fundulus grandis
… of tissues of F. grandis. The technique of one dimensional gel electrophoresis (1D-SDS-PAGE) was used to assess the effects of tissue sampling, flash frozen in liquid nitrogen versus immersion of fresh tissue in RNA later, for five tissues, liver, skeletal muscle, brain, gill, and heart, …