Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 63 for “"Two Hybrid System"”.
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Study of the HIV-1 Gene Regulatory Proteins TAT and REV by the Two-hybrid System in Yeast
… were cloned into plasmid vectors of the yeast two-hybrid system. This system enables large-scale screening of the members of a cDNA library for interaction with a protein of interest by the functional reconstitution of the GAL4 transcriptional activator.</p> <p>The search for Rev-interacting …
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Shall we dance? : investigating protein interactions implicated in Escherichia coli postreplicative mismatch repair using a bacterial two hybrid system
… to the integrity of DNA and multiple DNA repair systems to counteract these. MutS, MutL and MutH are integral elements of dam -directed repair (MMR), a mismatch recognition system involved in rectifying DNA replication errors. Moreover, MutS and MutL have been implicated in the effective …
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A two-color bacterial two-hybrid system for selecting sequence-specific DNA-binding proteins via fluorescence activated cell sorting
… a simple code. I then develop an experimental system (starting with a pre-existing bacterial two-hybrid selection system) which allows the selection of proteins based on their preference of one DNA site over another. I use this system to attempt to attenuate the affinity of a zinc finger …
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Interaction studies of the cellular prion protein
… native conditions. This was proven in the yeast two-hybrid system followed by the identification of two interaction domains. The influence of mutations and polymorphisms within the prion gene was investigated in the yeast two-hybrid system. This method is also an useful tool for the investigation …
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Isolation and partial characterization of cDNAs encoding polypeptides that interact with the capB gene products of Dictyostelium discoideum
… CABP1 encoded by the capB homolog capA. The GAL4 two-hybrid system was used to screen a Dictyostelium discoideum cDNA expression library to identify proteins that interacted with p34/31. The clones were tested to eliminate false positives. DNA sequencing analysis revealed that the cDNAs isolated …
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Funktionelle Analyse der Interaktion und Lokalisation von Replikationsfaktoren und replikationsrelevanten Proteinen in Mauszellen
… ORC1, ORC2 und CDC6 sowohl in Two Hybrid-Studien als auch in Immunpräzipitationen gezeigt werden. Darüberhinaus konnten signifikante Kolokalisationen dieser Proteine mit HP1a an heterochromatischen Regionen in murinen NIH3T3-Zellen nachgewiesen werden. Ebenfalls konnte hier …
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Das Sap47-Gen aus Drosophila melanogaster : Gezielte Mutagenisierung und Suche nach Interaktionspartnern
… SAP47 Protein zu identifizieren wurden ein Yeast-Two-Hybrid System und das "CytoTrap" Verfahren eingesetzt.
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Molekulare Analyse der Yersinien-Wirtszell Interaktion
… welches für ein Typ III Sekretion/Translokationsssystem und einige Virulenzfaktoren, sogenannte Yops (Yersinia outer proteins) kodiert. Die sechs bisher bekannten Effektor-Yops gelangen über das Sekretionssystem ins Zytoplasma von Makrophagen und Granulozyten, was in diesen zu einer Inhibition von …
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Structural and Functional Characterization of GAPR-1, a Mammalian Plant Pathogenesis-related Protein in Lipid-enriched Microdomains of the Golgi Complex
… by crosslink experiments and by the yeast two hybrid system. By affinity chromatography, GAPR-1 was shown to interact with three proteins: Nucleolin, Template activating factor α (TAFIα) and HSAPRIL. In the yeast two hybrid system, the interaction of GARP-1 with Nucleolin was confirmed and …
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Neue Interaktionspartner der MAPKAP-Kinasen 3pK und MK2
… zellulären Proliferation. HPH2 bindet im Hefe two-hybrid-System sowohl an 3pK als auch an MK2. Die Kinasen coimmunpräzipitieren auch mit den PcG-Proteinen. Weiterhin wurde festgestellt, daß nicht aktivierte und DNA-assoziierte 3pK, ähnlich wie Bmi1, in einem in vivo Repressionsassay als …
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Analysis of Protein and RNA Interactions of the HIV-1 rev Protein
… virion formation. We report identification of two yeast nucleopore proteins, NUP49 and NUP100, that have been found to interact with Rev <em>in vivo</em> using the yeast two-hybrid system. NUP49 and NUP100 contain GLFG repeats in their amino termini, but their carboxyl termini diverge. We have …
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Identifizierung neuer Interaktionsproteine des humanen Septins SEPT4 (hCDCrel-2) in fetalem Gehirn
… von SEPT4 in fetalem Gehirn mit dem Yeast Two-Hybrid System charakterisiert: SEPT8, SEPT11 und die Proteine Filamin B, MURR1 und die Ubiquitin-spezifische Protease 31. Es wurde in dieser Arbeit gezeigt, dass auch der N-Terminus von SEPT4 in Interaktionen mit Septinen und anderen Proteinen …
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Identifizierung und Charakterisierung von Interaktionen der Nichtstrukturproteine NS1 und NS2 des Respiratorischen Synzytialvirus mit Proteinen der Wirtszelle
… like Respiratory Syncytial Virus (RSV) encode two nonstructural proteins, NS1 and NS2. These two proteins cooperatively mediate type I interferon resistance and prevent induction of interferon in infected cells. Interactions of NS1 and NS2 proteins in every combination were shown by using the …
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Characterization of the Protein Phosphatase 2A Regulatory Subunit PR70
… subunit termed PR70 was identified in a yeast two hybrid screen with the DNA replication protein Cdc6 as bait. PR70 interacts with the PP2A core dimer and Cdc6 in vivo and in vitro. Biochemical approaches were used to identify regions and residues within PR70 that are important for mediating …
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Yeast Dynamin Functions with Clathrin at the Golgi
… Vps1 and clathrin was examined using a Yeast Two-Hybrid system, and found that Vps1 and clathrin indeed interact in vivo. Together, these findings demonstrate a functional relationship between Vps1 and clathrin, which is consistent with previous models whereby Vps1 mediates scission of …
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Novel Physical Interactions of Vps1 with Major Intracellular Traffic Regulators
… Vps1 with the eleven regulators, using a yeast two-hybrid system, and identified novel Vps1 binding partners, including Ypt6, Vps22, Vps24, and Vps36. The GTPase and middle domain of Vps1 appeared to have higher affinities to Ypt6 than the full-length Vps1, whereas the full length of Vps1 had a …
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Identification of binding protein with kinesin18B
… that interacts with kinesin18B using a yeast two-hybrid system, GST pull-down assay, immunoprecipitation. This protein is Microtubule-associated protein RP/EB family member 1 containing a calponin homology(CH) domain at their N terminus and an EB1-like C-terminal motif at their C terminus : …
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Identifikation und Charakterisierung von Interaktionspartnern des Zystennierenproteins DZIP1L
… of interaction partners of DZIP1L by a bacterial Two Hybrid system to further characterize the function and role of the protein in the cell. Coimmunoprecipitation experiments and immunfluorescence studies in transiently transfected COS7-, HEK293- and mIMCD-3- cells have been performed for …
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Identification and characterization of Risp
… for new Rev-interacting factors using the yeast two-hybrid system. In this work a new Rev-interacting protein has been identified, by screening a Jurkat T cell cDNA library. The protein was termed Risp (Rev-interacting shuttle protein), because it shuttles between the nuclear and the cytoplasmic …
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