Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"TurboID"”.
-
Directed evolution of TurboID for efficient proximity labeling in living cells and organisms
… a new proximity labeling method, called TurboID, that uses non-toxic labeling conditions and has high catalytic efficiency that allows its use in a wide variety of biological contexts. Here, we describe our use of yeast display-based directed evolution to engineer two promiscuous mutants …
-
Analysis of Human Interferon Signaling and its Regulation
… In the main part of this thesis, I applied TurboID-based proximity labeling to identify putative interactors of all seven canonical type I IFN signaling members, IFNAR1, IFNAR2, JAK1, TYK2, STAT1, STAT2, and IRF9, at various times post type I IFN stimulation. Using highly stringent selection …
-
An Optogenetic Toolbox for the Interrogation of Dynamic Events During Angiogenesis
… biotin proximity ligase termed LightR-TurboID. Fusion of LightR-TurboID to VE-Cadherin resulted in a construct capable of labelling known interacting partners with sub-minute resolution. Furthermore, the stability of biotinylation also enables the capture of weak or transient binders …
-
Identification and characterisation of novel interactors of surfactant protein C
… SFTPC-WT and -I73T proximity labelling using TurboID and identified labelled proteins by mass spectrometry. I discovered >100 proteins preferentially labelled by SFTPC-WT and therefore likely to be required for normal SFTPC trafficking. I undertook validation of biologically relevant hits by …
-
INTERACTOMICS FOR RICE FLOWERING: A PROXIMITY LABELLING APPROACH
… proteine. Il PL si basa sulla fusione della TurboID (TbID), una biotin-ligasi ottimizzata, alla proteina di interesse con lo scopo di marcarne gli interattori in vivo. L'identificazione del cosiddetto proteoma prossimale è resa possibile dalla precipitazione selettiva delle proteine …
-
Structural and Spatiotemporal Organisation of Bax and Apaf1 during Apoptosis
… biotinylation assay in living cells (BioID/TurboID) is presented, which allowed the identification of Bax cluster interactor proteins such as the fission proteins DRP1 and MFF and the mitochondrial matrix chaperone TRAP1. This demonstrates that Bax clusters might sequester various proteins …
-
AN INVESTIGATION OF THE MECHANISMS OF GROUCHO MEDIATED TRANSCRIPTIONAL REPRESSION
… as bait in the yeast 2-hybrid screen, and in a TurboID-based proximity labelling assay in Drosophila Kc167 cells. However, these approaches resulted in unfruitful finding novel interacting partners. Taking together all the evidence from this work I proposed a model of Gro-mediated repression, in …
-
Investigating FAM83H, a protein mutated in Amelogenesis Imperfecta
… techniques, including the recently described TurboID system, sheds more light on the components of these FAM83H speckles and generation of FAM83H-/- ameloblast-lineage cells (ALC) and U2OS osteosarcoma cells provide a good platform for further investigation of this protein.
-
Elucidation and characterisation of checkpoint kinase interactions with the replisome
DNA must be accurately and faithfully copied in each cell cycle by the process of DNA replication. During replication, cells must endure assaults from a myriad of exogenous and endogenous agents and events which can interfere with replication. Cells have evolved a surveillance mechanism called the …