Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Tunicamycin"”.
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Synthetic Studies on Tunicamycin Towards the Discovery of Antimetastatic Agents
The tunicamycins have been broadly applied in research of N-linked glycosylation and protein misfolding fields. The tunicamycins display antiproliferative activity against a wide range of cancer and healthy cell lines with narrow therapeutic index. Cytotoxicity of the tunicamycins is believed to be …
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Regulation of Pkr Activation by Pact and Trbp In Response to Stress Signals
… response to endoplasmic reticulum (ER) stressor tunicamycin. Our results establish that PACT is phosphorylated in response to tunicamycin, causing its increased association with PKR leading to PKR activation. Thus, our results uncover a novel functional role of PACT-mediated PKR activation in …
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Synthesis and evaluation of M.tb Glycosyltransferase (MshA) inhibitors
… (4 -34) which displayed th e highest potency. Tunicamycin which is a known glycosyltransferase inhibitor displayed the highest potency against M.tb H37Rv whole cells by inhibiting cell growth with a MIC 50 of 5 μg/mL . Tunicamycin inhibits the transfer of GlcNAc-1-P from UDP-GlcNAc to …
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The regulation of hypoxia-responsive gene expression by hydroxyl radicals and intracellular calcium
… Zielgene führt. Verschiedene Agentien darunter Tunicamycin, Brefeldin A und Thapsigargin wurden benutzt, um das ER in Stress zu versetzen. Dabei wurde gefunden, dass sowohl Tunicamycin als auch Brefeldin A die Hypoxie-induzierte HIF-1a Aktivierung aufheben. Thapsigargin, ein Hemmer der …
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Molecular Adaptation of Pancreatic Beta-Cell Function During Endoplasmic Reticulum Stress
… with the proteasome inhibitor lactacystin or by tunicamycin. Although the GSH/GSSG couple (indicative of redox state) was unaffected after lactacystin exposure in high glucose, lactacystin significantly decreased the GSH/GSSG couple in lower glucose (2.8 and 5.6 mM). Notably, high glucose …
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N-linked glycosylation at position ASN98 of the ALK1 receptor protein: relevance for ALK1 function and HHT pathogenesis
… server), (ii) a glycosylation inhibiting drug (tunicamycin), (iii) an in vitro enzymatic approach of glycosylation breakdown, and (iv) site-directed mutagenesis to identify the ASP residue glycosylated on ALK1. The bioinformatics software NetNGlyc predicted a N-linked glycosylation site on an …
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Characterization of The Role of Carma3 In Endoplasmic Reticulum Stress-Induced Nf-Κb Activation
… used ER stress inducers, thapsigargin and tunicamycin, to study the mechanism. We found that two caspase-recruitment domain (CARD)-containing proteins, CARMA3 and BCL10, play a crucial roles on ER stress-induced NF-κB activation by regulating IκBα kinase activity. Consistently, we observed …
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The unfolded protein response and HLA-B27 misfolding: implications for ankylosing spondylitis
… cells inefficiently induce a UPR in response to tunicamycin and that this has consequences for cell survival. However, inefficient activation of the UPR in HT1080 cells can be overcome by secondary signals, since co-administration of the tyrosine kinase inhibitor genistein leads to activation of …
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Endosomal Sorting Complex Required for Transport (ESCRT): Investigation of the expression and functional roles in neurodegenerative diseases
… MND cellular models such as NCS-34 cells using tunicamycin treatment. Altering the expression of TSG101 and CHMP2B demonstrated changes in TDP-43 and mTOR expression including altered autophagy markers such as LC3B and p62. Finally, knockout of SOCS3 enhances autophagy via TBK1 stabilisation. …
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Super Low Dose Endotoxin Exacerbates Low Grade Inflammation through Modulating Cell Stress and Decreasing Cellular Homeostatic Protein Expression
… endotoxin. In naïve cells, ER stressor such as tunicamycin (TM) not only will induce cellular stress and inflammation through JNK and NFkβ activation, but also will cause subsequent compensatory homeostasis through inducing homeostatic molecules such as XBP1 and GRP78/BiP. We observed that cells …
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Untersuchungen zu Bacillus cereus Enterotoxin-Komplexen auf zellulärer Ebene
… for HBL producers. After sensibilisation with tunicamycin Vero cells showed a substantial increased cytotoxic activity (63 – 132 %). The same effect could be observed in Fumonisin B pretreated cells for crude supernatants, but not for the Nhe B-/HBL+ preparation. Neither treatment with …
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Ire1 regulates cyp11a1 transcription to mediate steroidogenesis in the ovary
… were treated with and without forskolin, or tunicamycin and co-treated with the IRE1 endoribonuclease inhibitors MKC-4485 and MKC-3946. MKC-4485 and MKC-3946 potently inhibited XBP1 splicing but had no effect CYP11A1 up-regulation in response to forskolin stimulation. Additionally, we sought …
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NOVEL MECHANISMS FOR PROTEIN SYNTHESIS REGULATION DURING STRESS
… of protein synthesis after 24 or 48 hours of tunicamycin-induced ER stress, as compared to chronic ER stress, observed in the islets from Akita mice. We found that acute transient stress caused a decrease in protein synthesis in pancreatic islets compared to that in chronic ER stress. …
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Neuroprotective Effects of Sulforaphane and the Involvement of Autophagy
… protein levels dose-dependently. In addition, tunicamycin-induced ER-stress was significantly reduced by SFN, as shown using the ER-stress marker CHOP on protein and RNA levels. To intensify the research of SFN and autophagy, primary neuronal cells (PNCs) were developed from embryos from …
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Micro lipid droplet precursors of milk lipid globules
… After treatment of mammary epithelial cells with tunicamycin, butyrophilin content of this membrane is reduced. Thus a method for the study of the physiological role of this glycoprotein is proposed.
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Priming and processing glycosyltransferases in bacterial N-linked glycosylation pathways
… of PgIC, inspired by the nucleoside antibiotics tunicamycin and mureidomycin. In the final chapter, the enzymes in the N-linked protein glycosylation pathway in Campylobacter jejuni were evaluated for their tolerance for azide-modified UDP-sugar substrates. In vitro experiments were employed to …
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Biosynthesis and degradation of proteoglycans in cultured smooth muscle cells
… increased. Hyaluronic acid and the antibiotic Tunicamycin had no effect on the biosynthesis of sulphated macromolecules produced by the rat smooth muscle cells. However, p-nitrophenyl-β-D-xyloside increased by 10-fold the amount of radioactive sulphate incorporation into macromolecules in the …
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The Unfolded Protein Response Increases Production of Pro-Angiogenic Factors by Tumor Cell Lines
… of UPR inducing conditions (i.e., thapsigargin, tunicamycin, and no glucose) up-regulated VEGF, IL-8 and Angiogenin transcripts and extended these finding to a rat glioma line, a mouse fibroblast line and two human neuroblastoma lines. Our western blot and ELISA assay demonstrated the protein and …
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The Nuclear Pore Complex: Novel Glycoprotein Constituents
… of synthesis when p62 is soluble and cytosolic. Tunicamycin does not prevent glycosylation and the M<sub>r</sub> of p62 is not affected by endoglycosidase H. Thus, the addition of GlcNAc appears to be carried out by a cytoplasmically disposed transferase and is clearly distinct from the N- and …
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