Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Tubulogenesis"”.
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An analysis of the role of microtubules during tubulogenesis in Drosophila
… In order to ascertain their role in successful tubulogenesis I looked at both stable and newly polymerized MTs within the salivary gland placode. Apical constriction is accompanied by the loss of a centrosomal apically localised MT network and the appearance of a longitudinal acentrosomal MT …
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The role of the mechanical microenvironment in regulation of cholangiocyte specification and tubulogenesis
Emerging evidence highlights the influence of the extracellular matrix (ECM) on cell fate and functionality associated with ageing, development and disease (Segel et al. 2019; Bansaccal et al. 2023). Despite most studies emphasising ECM stiffness, recent insights suggest a cooperative role of ECM …
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Kidney tubulogenesis in human renal organoids: an investigation into the contribution of the microtubule-associated protein DCDC2
… the role of the cytoskeleton in MET and kidney tubulogenesis. I initially assessed and demonstrated that the human renal organoids match the key mesenchymal and epithelial stages seen in the human foetal kidney and published organoid data. Using analysis of both epithelial and mesenchymal …
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Signalling and function of the small Rho GTPase RhoJ in endothelial cells
… impairs endothelial cell (EC) migration and tubulogenesis, increases stress fibre (SF) and focal adhesion (FA) numbers. This work aimed to determine the intracellular localisation of RhoJ, identify its binding partners, test how it is activated and further explore its function in ECs. …
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Epithelial Tubular Fusion, Measuring Interconnection, and the Quest for Solving the Continuity Problem
… (HGF), which has known roles in cell migration, tubulogenesis, and cell proliferation. Addition of HGF robustly induced interconnection of adjacent spheroids. The proliferative effect of HGF neither promoted nor inhibited the interconnections. Additional studies with inhibitors of matrix …
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Role of p53 and its isoforms in the expression of FGF-2 and tumoral neovascularization
… inhibited endothelial cell - EC - migration and tubulogenesis. Furthermore, in the chicken chorioallantoïd membrane CAM, D133p53 knockdown gave rise to smaller tumours devoid of vessels, whereas, in mice, it strongly inhibited tumour growth. Interestingly, the double knockdown of p53 and D133p53 …
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Promotion and control of angiogenic activity in poly(ethylene glycol) diacrylate hydrogels for tissue engineering applications
… to promote endothelial cell proliferation and tubulogenesis in two and three dimensions in vitro at levels comparable to those achieved through signaling by VEGF. This work improves upon previous research on angiogenic growth factor release from tissue engineering matrices by showing that …
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Role of Sox9 In Uterine Gland Development and Disease Initiation
… us to peer into the genetic mechanism of tubulogenesis and branching morphogenesis; two processes that are disrupted in various adenocarcinomas (cancer derived from glands). We created 3D replicas of the epithelium lining the FRT using optical projection tomography and characterized UG …
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Exploring the transcriptomic landscape of the Drosophila melanogaster embryonic salivary gland.
… investigate this, I utilised a simple model of tubulogenesis found in the Drosophila melanogaster embryonic salivary gland. Once specified, two mirror-symmetric placodes internalise from a flat epithelium in a coordinated manner, form two narrow-lumen epithelial tubes on the inside and …
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A 3-dimensional vascularized cardiac tri-culture model using chitosan nanofiber scaffolds
… and migration and (2) Induction of endothelial tubulogenesis to recreate the vascularized architecture of the myocardium. The third aim was to investigate the effect of co-culturing cardiomyocytes with fibroblasts on cardiomyocytes' survival and contractility in the vascularized 3-D chitosan …
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Role of ANLN in post ischaemic angiogenesis
… A correlation between ANLN expression and tubulogenesis assay performance was observed associating ANLN with an early model of sprouting angiogenesis. A potential regulatory mechanism of ANLN by ZFP36 RNA binding proteins was identified in vascular inflammation and ischaemia in vivo and in …
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Developmental and Cellular Functions of Delta-Catenin
… gastrulation, neural crest migration and kidney tubulogenesis, phenotypes that were specific based upon rescue experiments. In biochemical and cellular assays, delta-catenin knock-down reduced cadherin levels and cell adhesion, and impaired activation of RhoA and Rac1, small GTPases that regulate …
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Quantitative Measurements of Force Distribution in Single and Multi Cellular Systems
… aggregates generated tubular shapes. So far, tubulogenesis in vitro was only inducible by adding growth factors to the culture medium.
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Exploring vasculogenic mimicry and anti-angiogenic therapy resistance within breast cancer patient-derived tissue xenograft models
… were enriched in extracellular matrix proteins, tubulogenesis, cell to cell adhesion and anticoagulation, all of which are processes which are known to be important within VM. Furthermore, genes associated with caveolae membranes were identified and in particular, caveolin-1 was found to be …
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EXC-5 Controls Intracellular Trafficking in Order to Maintain the Apical Structure of the C. elegans Excretory Canal
The goal of this work is to understand how cells form and maintain tubular shapes. The protein EXC-5 is necessary for a small tubular structure in Caenorhabditis elegans to maintain its shape. C. elegans is a small, easily manipulable, genetically tractable, and transparent nematode with a …
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ENDOTHELIAL- EPITHELIAL INTERACTIONS Blood vessels friends or foes?
Diabetes affects enormous amounts of patients. In type 1 diabetes the insulin producing β cells in the islet of Langerhans are destroyed by the immune system. The β cells, which are located in the pancreas, regulate blood glucose levels. The lack of insulin production requires administration of …